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The putative compatible solute-binding protein ProX from Mycobacterium tuberculosis H37Rv: biochemical characterization and crystallographic data

In Mycobacterium tuberculosis, the proX gene encodes a putative compatible solute-binding protein (MtProX). However, it was found through sequence alignment that the MtProX protein has very different ligand-binding residues compared with other compatible solute-binding proteins, implying that MtProX...

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Autores principales: Zhao, Jian-Hong, Chen, Jiang-Huai, Wang, Yong, Wang, Zhi-Ping, He, Yong-Xing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5894108/
https://www.ncbi.nlm.nih.gov/pubmed/29633971
http://dx.doi.org/10.1107/S2053230X18003771
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author Zhao, Jian-Hong
Chen, Jiang-Huai
Wang, Yong
Wang, Zhi-Ping
He, Yong-Xing
author_facet Zhao, Jian-Hong
Chen, Jiang-Huai
Wang, Yong
Wang, Zhi-Ping
He, Yong-Xing
author_sort Zhao, Jian-Hong
collection PubMed
description In Mycobacterium tuberculosis, the proX gene encodes a putative compatible solute-binding protein (MtProX). However, it was found through sequence alignment that the MtProX protein has very different ligand-binding residues compared with other compatible solute-binding proteins, implying that MtProX may bind to ligands that are as yet uncharacterized. In this work, it was demonstrated that MtProX binds to polyphenols such as phloretin, monoacetylphloroglucinol and 2,4-dihydroxyacetophloroglucinol with dissociation constants between 20 and 70 µM. Crystals of MtProX were obtained using a precipitant consisting of 0.2 M NaCl, 0.1 M Tris pH 8.5, 25%(w/v) polyethylene glycol 3350. The crystals diffracted to 2.10 Å resolution and belonged to space group P4(3)2(1)2, with unit-cell parameters a = b = 90.17, c = 161.92 Å, α = β = γ = 90.0°. Assuming the presence of two MtProX molecules in the asymmetric unit, the Matthews coefficient was calculated to be 2.74 Å(3) Da(−1), which corresponds to a solvent content of 55%.
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spelling pubmed-58941082018-04-13 The putative compatible solute-binding protein ProX from Mycobacterium tuberculosis H37Rv: biochemical characterization and crystallographic data Zhao, Jian-Hong Chen, Jiang-Huai Wang, Yong Wang, Zhi-Ping He, Yong-Xing Acta Crystallogr F Struct Biol Commun Research Communications In Mycobacterium tuberculosis, the proX gene encodes a putative compatible solute-binding protein (MtProX). However, it was found through sequence alignment that the MtProX protein has very different ligand-binding residues compared with other compatible solute-binding proteins, implying that MtProX may bind to ligands that are as yet uncharacterized. In this work, it was demonstrated that MtProX binds to polyphenols such as phloretin, monoacetylphloroglucinol and 2,4-dihydroxyacetophloroglucinol with dissociation constants between 20 and 70 µM. Crystals of MtProX were obtained using a precipitant consisting of 0.2 M NaCl, 0.1 M Tris pH 8.5, 25%(w/v) polyethylene glycol 3350. The crystals diffracted to 2.10 Å resolution and belonged to space group P4(3)2(1)2, with unit-cell parameters a = b = 90.17, c = 161.92 Å, α = β = γ = 90.0°. Assuming the presence of two MtProX molecules in the asymmetric unit, the Matthews coefficient was calculated to be 2.74 Å(3) Da(−1), which corresponds to a solvent content of 55%. International Union of Crystallography 2018-03-23 /pmc/articles/PMC5894108/ /pubmed/29633971 http://dx.doi.org/10.1107/S2053230X18003771 Text en © Zhao et al. 2018 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/2.0/uk/
spellingShingle Research Communications
Zhao, Jian-Hong
Chen, Jiang-Huai
Wang, Yong
Wang, Zhi-Ping
He, Yong-Xing
The putative compatible solute-binding protein ProX from Mycobacterium tuberculosis H37Rv: biochemical characterization and crystallographic data
title The putative compatible solute-binding protein ProX from Mycobacterium tuberculosis H37Rv: biochemical characterization and crystallographic data
title_full The putative compatible solute-binding protein ProX from Mycobacterium tuberculosis H37Rv: biochemical characterization and crystallographic data
title_fullStr The putative compatible solute-binding protein ProX from Mycobacterium tuberculosis H37Rv: biochemical characterization and crystallographic data
title_full_unstemmed The putative compatible solute-binding protein ProX from Mycobacterium tuberculosis H37Rv: biochemical characterization and crystallographic data
title_short The putative compatible solute-binding protein ProX from Mycobacterium tuberculosis H37Rv: biochemical characterization and crystallographic data
title_sort putative compatible solute-binding protein prox from mycobacterium tuberculosis h37rv: biochemical characterization and crystallographic data
topic Research Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5894108/
https://www.ncbi.nlm.nih.gov/pubmed/29633971
http://dx.doi.org/10.1107/S2053230X18003771
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