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Detection of Actinobacillus Pleuropneumoniae ApxIV Toxin Antibody in Serum and Oral Fluid Specimens from Pigs Inoculated Under Experimental Conditions

INTRODUCTION: The prevention and control of Actinobacillus pleuropneumoniae in commercial production settings is based on serological monitoring. Enzyme-linked immunosorbent assays (ELISAs) have been developed to detect specific antibodies against a variety of A. pleuropneumoniae antigens, including...

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Autores principales: González, Wendy, Giménez-Lirola, Luis G., Holmes, Ashley, Lizano, Sergio, Goodell, Christa, Poonsuk, Korakrit, Sitthicharoenchai, Panchan, Sun, Yaxuan, Zimmerman, Jeffrey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: De Gruyter Open 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5894388/
https://www.ncbi.nlm.nih.gov/pubmed/29978069
http://dx.doi.org/10.1515/jvetres-2017-0021
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author González, Wendy
Giménez-Lirola, Luis G.
Holmes, Ashley
Lizano, Sergio
Goodell, Christa
Poonsuk, Korakrit
Sitthicharoenchai, Panchan
Sun, Yaxuan
Zimmerman, Jeffrey
author_facet González, Wendy
Giménez-Lirola, Luis G.
Holmes, Ashley
Lizano, Sergio
Goodell, Christa
Poonsuk, Korakrit
Sitthicharoenchai, Panchan
Sun, Yaxuan
Zimmerman, Jeffrey
author_sort González, Wendy
collection PubMed
description INTRODUCTION: The prevention and control of Actinobacillus pleuropneumoniae in commercial production settings is based on serological monitoring. Enzyme-linked immunosorbent assays (ELISAs) have been developed to detect specific antibodies against a variety of A. pleuropneumoniae antigens, including long-chain lipopolysaccharides (LPS) and the ApxIV toxin, a repeats-in-toxin (RTX) exotoxin unique to A. pleuropneumoniae and produced by all serovars. The objective of this study was to describe ApxIV antibody responses in serum and oral fluid of pigs. MATERIAL AND METHODS: Four groups of pigs (six pigs per group) were inoculated with A. pleuropneumoniae serovars 1, 5, 7, or 12. Weekly serum samples and daily oral fluid samples were collected from individual pigs for 56 days post inoculation (DPI) and tested by LPS and ApxIV ELISAs. The ApxIV ELISA was run in three formats to detect immunlgobulins M, G, and A (IgM, IgG and IgA) while the LPS ELISA detected only IgG. RESULTS: All pigs inoculated with A. pleuropneumoniae serovars 1 and 7 were LPS ELISA serum antibody positive from DPI 14 to 56. A transient and weak LPS ELISA antibody response was observed in pigs inoculated with serovar 5 and a single antibody positive pig was observed in serovar 12 at ≥35 DPI. Notably, ApxIV serum and oral fluid antibody responses in pig inoculated with serovars 1 and 7 reflected the patterns observed for LPS antibody, albeit with a 14 to 21 day delay. CONCLUSION: This work suggests that ELISAs based on ApxIV antibody detection in oral fluid samples could be effective in population monitoring for A. pleuropneumoniae.
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spelling pubmed-58943882018-07-05 Detection of Actinobacillus Pleuropneumoniae ApxIV Toxin Antibody in Serum and Oral Fluid Specimens from Pigs Inoculated Under Experimental Conditions González, Wendy Giménez-Lirola, Luis G. Holmes, Ashley Lizano, Sergio Goodell, Christa Poonsuk, Korakrit Sitthicharoenchai, Panchan Sun, Yaxuan Zimmerman, Jeffrey J Vet Res Research Article INTRODUCTION: The prevention and control of Actinobacillus pleuropneumoniae in commercial production settings is based on serological monitoring. Enzyme-linked immunosorbent assays (ELISAs) have been developed to detect specific antibodies against a variety of A. pleuropneumoniae antigens, including long-chain lipopolysaccharides (LPS) and the ApxIV toxin, a repeats-in-toxin (RTX) exotoxin unique to A. pleuropneumoniae and produced by all serovars. The objective of this study was to describe ApxIV antibody responses in serum and oral fluid of pigs. MATERIAL AND METHODS: Four groups of pigs (six pigs per group) were inoculated with A. pleuropneumoniae serovars 1, 5, 7, or 12. Weekly serum samples and daily oral fluid samples were collected from individual pigs for 56 days post inoculation (DPI) and tested by LPS and ApxIV ELISAs. The ApxIV ELISA was run in three formats to detect immunlgobulins M, G, and A (IgM, IgG and IgA) while the LPS ELISA detected only IgG. RESULTS: All pigs inoculated with A. pleuropneumoniae serovars 1 and 7 were LPS ELISA serum antibody positive from DPI 14 to 56. A transient and weak LPS ELISA antibody response was observed in pigs inoculated with serovar 5 and a single antibody positive pig was observed in serovar 12 at ≥35 DPI. Notably, ApxIV serum and oral fluid antibody responses in pig inoculated with serovars 1 and 7 reflected the patterns observed for LPS antibody, albeit with a 14 to 21 day delay. CONCLUSION: This work suggests that ELISAs based on ApxIV antibody detection in oral fluid samples could be effective in population monitoring for A. pleuropneumoniae. De Gruyter Open 2017-12-06 /pmc/articles/PMC5894388/ /pubmed/29978069 http://dx.doi.org/10.1515/jvetres-2017-0021 Text en © 2017 W. González al http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article distributed under the Creative Commons Attribution-NonCommercial-NoDerivs license
spellingShingle Research Article
González, Wendy
Giménez-Lirola, Luis G.
Holmes, Ashley
Lizano, Sergio
Goodell, Christa
Poonsuk, Korakrit
Sitthicharoenchai, Panchan
Sun, Yaxuan
Zimmerman, Jeffrey
Detection of Actinobacillus Pleuropneumoniae ApxIV Toxin Antibody in Serum and Oral Fluid Specimens from Pigs Inoculated Under Experimental Conditions
title Detection of Actinobacillus Pleuropneumoniae ApxIV Toxin Antibody in Serum and Oral Fluid Specimens from Pigs Inoculated Under Experimental Conditions
title_full Detection of Actinobacillus Pleuropneumoniae ApxIV Toxin Antibody in Serum and Oral Fluid Specimens from Pigs Inoculated Under Experimental Conditions
title_fullStr Detection of Actinobacillus Pleuropneumoniae ApxIV Toxin Antibody in Serum and Oral Fluid Specimens from Pigs Inoculated Under Experimental Conditions
title_full_unstemmed Detection of Actinobacillus Pleuropneumoniae ApxIV Toxin Antibody in Serum and Oral Fluid Specimens from Pigs Inoculated Under Experimental Conditions
title_short Detection of Actinobacillus Pleuropneumoniae ApxIV Toxin Antibody in Serum and Oral Fluid Specimens from Pigs Inoculated Under Experimental Conditions
title_sort detection of actinobacillus pleuropneumoniae apxiv toxin antibody in serum and oral fluid specimens from pigs inoculated under experimental conditions
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5894388/
https://www.ncbi.nlm.nih.gov/pubmed/29978069
http://dx.doi.org/10.1515/jvetres-2017-0021
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