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Detection of Avian Reoviruses in Wild Birds in Poland

INTRODUCTION: The purpose of this study was to determine the occurrence of avian reovirus (ARV) infections in wild birds in Poland and attempt to propagate the selected ARV strains in chicken embryo kidney (CEK) cells or chicken SPF embryos. MATERIAL AND METHODS: The study included 192 wild birds re...

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Autores principales: Styś-Fijoł, Natalia, Kozdruń, Wojciech, Czekaj, Hanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: De Gruyter Open 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5894429/
https://www.ncbi.nlm.nih.gov/pubmed/29978079
http://dx.doi.org/10.1515/jvetres-2017-0033
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author Styś-Fijoł, Natalia
Kozdruń, Wojciech
Czekaj, Hanna
author_facet Styś-Fijoł, Natalia
Kozdruń, Wojciech
Czekaj, Hanna
author_sort Styś-Fijoł, Natalia
collection PubMed
description INTRODUCTION: The purpose of this study was to determine the occurrence of avian reovirus (ARV) infections in wild birds in Poland and attempt to propagate the selected ARV strains in chicken embryo kidney (CEK) cells or chicken SPF embryos. MATERIAL AND METHODS: The study included 192 wild birds representing 32 species, collected between 2014 and 2016. A part of the S4 segment encoding the σNS protein of avian reoviruses (ARVs) isolated from different species of wild birds from that period was amplified. RESULTS: The presence of ARV was demonstrated in 58 (30.2%) wild birds belonging to nine orders. The isolated strains were propagated in chicken embryos by yolk sac inoculation, and CPE was induced in the infected CEK monolayer. Agar gel precipitation showed that two ARV isolates from rock pigeon and mute swan shared a common group-specific antigen with chicken reovirus S1133. Specific products of predicted size were found in two ARV isolates from the chicken embryo passage and 13 ARVs isolated from CEK cells. CONCLUSION: The study indicates the high prevalence of ARV among wild birds in Poland and its possible transmission to farmed birds.
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spelling pubmed-58944292018-07-05 Detection of Avian Reoviruses in Wild Birds in Poland Styś-Fijoł, Natalia Kozdruń, Wojciech Czekaj, Hanna J Vet Res Research Article INTRODUCTION: The purpose of this study was to determine the occurrence of avian reovirus (ARV) infections in wild birds in Poland and attempt to propagate the selected ARV strains in chicken embryo kidney (CEK) cells or chicken SPF embryos. MATERIAL AND METHODS: The study included 192 wild birds representing 32 species, collected between 2014 and 2016. A part of the S4 segment encoding the σNS protein of avian reoviruses (ARVs) isolated from different species of wild birds from that period was amplified. RESULTS: The presence of ARV was demonstrated in 58 (30.2%) wild birds belonging to nine orders. The isolated strains were propagated in chicken embryos by yolk sac inoculation, and CPE was induced in the infected CEK monolayer. Agar gel precipitation showed that two ARV isolates from rock pigeon and mute swan shared a common group-specific antigen with chicken reovirus S1133. Specific products of predicted size were found in two ARV isolates from the chicken embryo passage and 13 ARVs isolated from CEK cells. CONCLUSION: The study indicates the high prevalence of ARV among wild birds in Poland and its possible transmission to farmed birds. De Gruyter Open 2017-09-19 /pmc/articles/PMC5894429/ /pubmed/29978079 http://dx.doi.org/10.1515/jvetres-2017-0033 Text en © 2017 N. Styś-Fijoł et al. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article distributed under the Creative Commons Attribution-NonCommercial-NoDerivs license
spellingShingle Research Article
Styś-Fijoł, Natalia
Kozdruń, Wojciech
Czekaj, Hanna
Detection of Avian Reoviruses in Wild Birds in Poland
title Detection of Avian Reoviruses in Wild Birds in Poland
title_full Detection of Avian Reoviruses in Wild Birds in Poland
title_fullStr Detection of Avian Reoviruses in Wild Birds in Poland
title_full_unstemmed Detection of Avian Reoviruses in Wild Birds in Poland
title_short Detection of Avian Reoviruses in Wild Birds in Poland
title_sort detection of avian reoviruses in wild birds in poland
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5894429/
https://www.ncbi.nlm.nih.gov/pubmed/29978079
http://dx.doi.org/10.1515/jvetres-2017-0033
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