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Optimization of the cydex blue assay: A one-step colorimetric protein assay using cyclodextrins and compatible with detergents and reducers
Sodium dodecyl sulfate electrophoresis (SDS) is a protein separation technique widely used, for example, prior to immunoblotting. Samples are usually prepared in a buffer containing both high concentrations of reducers and high concentrations of SDS. This conjunction renders the samples incompatible...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5895047/ https://www.ncbi.nlm.nih.gov/pubmed/29641569 http://dx.doi.org/10.1371/journal.pone.0195755 |
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author | Rabilloud, Thierry |
author_facet | Rabilloud, Thierry |
author_sort | Rabilloud, Thierry |
collection | PubMed |
description | Sodium dodecyl sulfate electrophoresis (SDS) is a protein separation technique widely used, for example, prior to immunoblotting. Samples are usually prepared in a buffer containing both high concentrations of reducers and high concentrations of SDS. This conjunction renders the samples incompatible with common protein assays. By chelating the SDS, cyclodextrins make the use of simple, dye-based colorimetric assays possible. In this paper, we describe the optimization of the assay, focussing on the cyclodextrin/SDS ratio and the use of commercial assay reagents. The adaptation of the assay to a microplate format and using other detergent-containing conventional extraction buffers is also described. |
format | Online Article Text |
id | pubmed-5895047 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-58950472018-05-04 Optimization of the cydex blue assay: A one-step colorimetric protein assay using cyclodextrins and compatible with detergents and reducers Rabilloud, Thierry PLoS One Research Article Sodium dodecyl sulfate electrophoresis (SDS) is a protein separation technique widely used, for example, prior to immunoblotting. Samples are usually prepared in a buffer containing both high concentrations of reducers and high concentrations of SDS. This conjunction renders the samples incompatible with common protein assays. By chelating the SDS, cyclodextrins make the use of simple, dye-based colorimetric assays possible. In this paper, we describe the optimization of the assay, focussing on the cyclodextrin/SDS ratio and the use of commercial assay reagents. The adaptation of the assay to a microplate format and using other detergent-containing conventional extraction buffers is also described. Public Library of Science 2018-04-11 /pmc/articles/PMC5895047/ /pubmed/29641569 http://dx.doi.org/10.1371/journal.pone.0195755 Text en © 2018 Thierry Rabilloud http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Rabilloud, Thierry Optimization of the cydex blue assay: A one-step colorimetric protein assay using cyclodextrins and compatible with detergents and reducers |
title | Optimization of the cydex blue assay: A one-step colorimetric protein assay using cyclodextrins and compatible with detergents and reducers |
title_full | Optimization of the cydex blue assay: A one-step colorimetric protein assay using cyclodextrins and compatible with detergents and reducers |
title_fullStr | Optimization of the cydex blue assay: A one-step colorimetric protein assay using cyclodextrins and compatible with detergents and reducers |
title_full_unstemmed | Optimization of the cydex blue assay: A one-step colorimetric protein assay using cyclodextrins and compatible with detergents and reducers |
title_short | Optimization of the cydex blue assay: A one-step colorimetric protein assay using cyclodextrins and compatible with detergents and reducers |
title_sort | optimization of the cydex blue assay: a one-step colorimetric protein assay using cyclodextrins and compatible with detergents and reducers |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5895047/ https://www.ncbi.nlm.nih.gov/pubmed/29641569 http://dx.doi.org/10.1371/journal.pone.0195755 |
work_keys_str_mv | AT rabilloudthierry optimizationofthecydexblueassayaonestepcolorimetricproteinassayusingcyclodextrinsandcompatiblewithdetergentsandreducers |