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Proteomic profiling of human cancer pseudopodia for the identification of anti-metastatic drug candidates
Cancer metastasis causes approximately 90% of all cancer-related death and independent of the advancement of cancer therapy, a majority of late stage patients suffers from metastatic cancer. Metastasis implies cancer cell migration and invasion throughout the body. Migration requires the formation o...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5895739/ https://www.ncbi.nlm.nih.gov/pubmed/29643415 http://dx.doi.org/10.1038/s41598-018-24256-8 |
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author | Choi, Sunkyu Bhagwat, Aditya M. Al Mismar, Rasha Goswami, Neha Ben Hamidane, Hisham Sun, Lu Graumann, Johannes |
author_facet | Choi, Sunkyu Bhagwat, Aditya M. Al Mismar, Rasha Goswami, Neha Ben Hamidane, Hisham Sun, Lu Graumann, Johannes |
author_sort | Choi, Sunkyu |
collection | PubMed |
description | Cancer metastasis causes approximately 90% of all cancer-related death and independent of the advancement of cancer therapy, a majority of late stage patients suffers from metastatic cancer. Metastasis implies cancer cell migration and invasion throughout the body. Migration requires the formation of pseudopodia in the direction of movement, but a detailed understanding of this process and accordingly strategies of prevention remain elusive. Here, we use quantitative proteomic profiling of human cancer pseudopodia to examine this mechanisms essential to metastasis formation, and identify potential candidates for pharmacological interference with the process. We demonstrate that Prohibitins (PHBs) are significantly enriched in the pseudopodia fraction derived from cancer cells, and knockdown of PHBs, as well as their chemical inhibition through Rocaglamide (Roc-A), efficiently reduces cancer cell migration. |
format | Online Article Text |
id | pubmed-5895739 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-58957392018-04-20 Proteomic profiling of human cancer pseudopodia for the identification of anti-metastatic drug candidates Choi, Sunkyu Bhagwat, Aditya M. Al Mismar, Rasha Goswami, Neha Ben Hamidane, Hisham Sun, Lu Graumann, Johannes Sci Rep Article Cancer metastasis causes approximately 90% of all cancer-related death and independent of the advancement of cancer therapy, a majority of late stage patients suffers from metastatic cancer. Metastasis implies cancer cell migration and invasion throughout the body. Migration requires the formation of pseudopodia in the direction of movement, but a detailed understanding of this process and accordingly strategies of prevention remain elusive. Here, we use quantitative proteomic profiling of human cancer pseudopodia to examine this mechanisms essential to metastasis formation, and identify potential candidates for pharmacological interference with the process. We demonstrate that Prohibitins (PHBs) are significantly enriched in the pseudopodia fraction derived from cancer cells, and knockdown of PHBs, as well as their chemical inhibition through Rocaglamide (Roc-A), efficiently reduces cancer cell migration. Nature Publishing Group UK 2018-04-11 /pmc/articles/PMC5895739/ /pubmed/29643415 http://dx.doi.org/10.1038/s41598-018-24256-8 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Choi, Sunkyu Bhagwat, Aditya M. Al Mismar, Rasha Goswami, Neha Ben Hamidane, Hisham Sun, Lu Graumann, Johannes Proteomic profiling of human cancer pseudopodia for the identification of anti-metastatic drug candidates |
title | Proteomic profiling of human cancer pseudopodia for the identification of anti-metastatic drug candidates |
title_full | Proteomic profiling of human cancer pseudopodia for the identification of anti-metastatic drug candidates |
title_fullStr | Proteomic profiling of human cancer pseudopodia for the identification of anti-metastatic drug candidates |
title_full_unstemmed | Proteomic profiling of human cancer pseudopodia for the identification of anti-metastatic drug candidates |
title_short | Proteomic profiling of human cancer pseudopodia for the identification of anti-metastatic drug candidates |
title_sort | proteomic profiling of human cancer pseudopodia for the identification of anti-metastatic drug candidates |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5895739/ https://www.ncbi.nlm.nih.gov/pubmed/29643415 http://dx.doi.org/10.1038/s41598-018-24256-8 |
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