Cargando…
Altered expression of microRNA during fracture healing in diabetic rats
OBJECTIVES: Diabetes mellitus (DM) is known to impair fracture healing. Increasing evidence suggests that some microRNA (miRNA) is involved in the pathophysiology of diabetes and its complications. We hypothesized that the functions of miRNA and changes to their patterns of expression may be implica...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5895942/ https://www.ncbi.nlm.nih.gov/pubmed/29437637 http://dx.doi.org/10.1302/2046-3758.72.BJR-2017-0082.R1 |
_version_ | 1783313745870585856 |
---|---|
author | Takahara, S. Lee, S. Y. Iwakura, T. Oe, K. Fukui, T. Okumachi, E. Waki, T. Arakura, M. Sakai, Y. Nishida, K. Kuroda, R. Niikura, T. |
author_facet | Takahara, S. Lee, S. Y. Iwakura, T. Oe, K. Fukui, T. Okumachi, E. Waki, T. Arakura, M. Sakai, Y. Nishida, K. Kuroda, R. Niikura, T. |
author_sort | Takahara, S. |
collection | PubMed |
description | OBJECTIVES: Diabetes mellitus (DM) is known to impair fracture healing. Increasing evidence suggests that some microRNA (miRNA) is involved in the pathophysiology of diabetes and its complications. We hypothesized that the functions of miRNA and changes to their patterns of expression may be implicated in the pathogenesis of impaired fracture healing in DM. METHODS: Closed transverse fractures were created in the femurs of 116 rats, with half assigned to the DM group and half assigned to the control group. Rats with DM were induced by a single intraperitoneal injection of streptozotocin. At post-fracture days five, seven, 11, 14, 21, and 28, miRNA was extracted from the newly generated tissue at the fracture site. Microarray analysis was performed with miRNA samples from each group on post-fracture days five and 11. For further analysis, real-time polymerase chain reaction (PCR) analysis was performed at each timepoint. RESULTS: Microarray analysis showed that there were 14 miRNAs at day five and 17 miRNAs at day 11, with a greater than twofold change in the DM group compared with the control group. Among these types of miRNA, five were selected based on a comparative and extended literature review. Real-time PCR analysis revealed that five types of miRNA (miR-140-3p, miR-140-5p, miR-181a-1-3p, miR-210-3p, and miR-222-3p) were differentially expressed with changing patterns of expression during fracture healing in diabetic rats compared with controls. CONCLUSIONS: Our findings provide information to further understand the pathology of impaired fracture healing in a diabetic rat model. These results may allow the potential development of molecular therapy using miRNA for the treatment of impaired fracture healing in patients with DM. Cite this article: S. Takahara, S. Y. Lee, T. Iwakura, K. Oe, T. Fukui, E. Okumachi, T. Waki, M. Arakura, Y. Sakai, K. Nishida, R. Kuroda, T. Niikura. Altered expression of microRNA during fracture healing in diabetic rats. Bone Joint Res 2018;7:139–147. DOI: 10.1302/2046-3758.72.BJR-2017-0082.R1. |
format | Online Article Text |
id | pubmed-5895942 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
record_format | MEDLINE/PubMed |
spelling | pubmed-58959422018-04-20 Altered expression of microRNA during fracture healing in diabetic rats Takahara, S. Lee, S. Y. Iwakura, T. Oe, K. Fukui, T. Okumachi, E. Waki, T. Arakura, M. Sakai, Y. Nishida, K. Kuroda, R. Niikura, T. Bone Joint Res Bone Biology OBJECTIVES: Diabetes mellitus (DM) is known to impair fracture healing. Increasing evidence suggests that some microRNA (miRNA) is involved in the pathophysiology of diabetes and its complications. We hypothesized that the functions of miRNA and changes to their patterns of expression may be implicated in the pathogenesis of impaired fracture healing in DM. METHODS: Closed transverse fractures were created in the femurs of 116 rats, with half assigned to the DM group and half assigned to the control group. Rats with DM were induced by a single intraperitoneal injection of streptozotocin. At post-fracture days five, seven, 11, 14, 21, and 28, miRNA was extracted from the newly generated tissue at the fracture site. Microarray analysis was performed with miRNA samples from each group on post-fracture days five and 11. For further analysis, real-time polymerase chain reaction (PCR) analysis was performed at each timepoint. RESULTS: Microarray analysis showed that there were 14 miRNAs at day five and 17 miRNAs at day 11, with a greater than twofold change in the DM group compared with the control group. Among these types of miRNA, five were selected based on a comparative and extended literature review. Real-time PCR analysis revealed that five types of miRNA (miR-140-3p, miR-140-5p, miR-181a-1-3p, miR-210-3p, and miR-222-3p) were differentially expressed with changing patterns of expression during fracture healing in diabetic rats compared with controls. CONCLUSIONS: Our findings provide information to further understand the pathology of impaired fracture healing in a diabetic rat model. These results may allow the potential development of molecular therapy using miRNA for the treatment of impaired fracture healing in patients with DM. Cite this article: S. Takahara, S. Y. Lee, T. Iwakura, K. Oe, T. Fukui, E. Okumachi, T. Waki, M. Arakura, Y. Sakai, K. Nishida, R. Kuroda, T. Niikura. Altered expression of microRNA during fracture healing in diabetic rats. Bone Joint Res 2018;7:139–147. DOI: 10.1302/2046-3758.72.BJR-2017-0082.R1. 2018-04-12 /pmc/articles/PMC5895942/ /pubmed/29437637 http://dx.doi.org/10.1302/2046-3758.72.BJR-2017-0082.R1 Text en © 2018 Takahara et al. This is an open-access article distributed under the terms of the Creative Commons Attributions licence (CC-BY-NC), which permits unrestricted use, distribution, and reproduction in any medium, but not for commercial gain, provided the original author and source are credited. |
spellingShingle | Bone Biology Takahara, S. Lee, S. Y. Iwakura, T. Oe, K. Fukui, T. Okumachi, E. Waki, T. Arakura, M. Sakai, Y. Nishida, K. Kuroda, R. Niikura, T. Altered expression of microRNA during fracture healing in diabetic rats |
title | Altered expression of microRNA during fracture healing in diabetic rats |
title_full | Altered expression of microRNA during fracture healing in diabetic rats |
title_fullStr | Altered expression of microRNA during fracture healing in diabetic rats |
title_full_unstemmed | Altered expression of microRNA during fracture healing in diabetic rats |
title_short | Altered expression of microRNA during fracture healing in diabetic rats |
title_sort | altered expression of microrna during fracture healing in diabetic rats |
topic | Bone Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5895942/ https://www.ncbi.nlm.nih.gov/pubmed/29437637 http://dx.doi.org/10.1302/2046-3758.72.BJR-2017-0082.R1 |
work_keys_str_mv | AT takaharas alteredexpressionofmicrornaduringfracturehealingindiabeticrats AT leesy alteredexpressionofmicrornaduringfracturehealingindiabeticrats AT iwakurat alteredexpressionofmicrornaduringfracturehealingindiabeticrats AT oek alteredexpressionofmicrornaduringfracturehealingindiabeticrats AT fukuit alteredexpressionofmicrornaduringfracturehealingindiabeticrats AT okumachie alteredexpressionofmicrornaduringfracturehealingindiabeticrats AT wakit alteredexpressionofmicrornaduringfracturehealingindiabeticrats AT arakuram alteredexpressionofmicrornaduringfracturehealingindiabeticrats AT sakaiy alteredexpressionofmicrornaduringfracturehealingindiabeticrats AT nishidak alteredexpressionofmicrornaduringfracturehealingindiabeticrats AT kurodar alteredexpressionofmicrornaduringfracturehealingindiabeticrats AT niikurat alteredexpressionofmicrornaduringfracturehealingindiabeticrats |