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Antiproliferative activities and phenolic acid content of water and ethanolic extracts of the powdered formula of Houttuynia cordata Thunb. fermented broth and Phyllanthus emblica Linn. fruit

BACKGROUND: Houttuynia cordata Thunb. and Phyllanthus emblica Linn. are native plants with medicinal and nutritive significance in Asia. The present study was aimed at evaluating antiproliferative effects on human cancer cell lines and identifying the phenolic acid composition of water and ethanolic...

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Autores principales: Kumnerdkhonkaen, Piyawan, Saenglee, Somprasong, Asgar, Md. Ali, Senawong, Gulsiri, Khongsukwiwat, Kanoknan, Senawong, Thanaset
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5896045/
https://www.ncbi.nlm.nih.gov/pubmed/29642867
http://dx.doi.org/10.1186/s12906-018-2185-x
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author Kumnerdkhonkaen, Piyawan
Saenglee, Somprasong
Asgar, Md. Ali
Senawong, Gulsiri
Khongsukwiwat, Kanoknan
Senawong, Thanaset
author_facet Kumnerdkhonkaen, Piyawan
Saenglee, Somprasong
Asgar, Md. Ali
Senawong, Gulsiri
Khongsukwiwat, Kanoknan
Senawong, Thanaset
author_sort Kumnerdkhonkaen, Piyawan
collection PubMed
description BACKGROUND: Houttuynia cordata Thunb. and Phyllanthus emblica Linn. are native plants with medicinal and nutritive significance in Asia. The present study was aimed at evaluating antiproliferative effects on human cancer cell lines and identifying the phenolic acid composition of water and ethanolic extracts of the powdered formula of H. cordata fermented broth and P. emblica fruit. METHODS: Anticancer activity of the extracts was evaluated against HeLa, HT29, HCT116, MCF7 and Jurkat cells using an MTT assay and flow cytometric analysis of apoptosis induction and cell cycle arrest. Reverse phase HPLC was exploited for identification and quantification of some phenolic acids. RESULTS: MTT assay showed that both water and ethanolic extracts significantly decreased the viability of cancer cells in a dose- and time-dependent fashion. Based on the IC(50) values, ethanolic extract (IC(50) values = 0.12–0.65 mg/mL) was more cytotoxic than water extract (IC(50) values = 0.22–0.85 mg/mL) and Jurkat cells were the most sensitive to both extracts (IC(50) values = 0.12–0.69 mg/mL). The underlying mechanism for antiproliferative activity was apoptosis induction, especially in HT29, HCT116, MCF7 and Jurkat cells. HT29 cells were the most sensitive to extract-induced apoptosis. Ethanolic extract was more effective at inducing apoptosis than water extract. Moreover, cell cycle arrest was found to be another mechanism behind growth inhibition in Jurkat and HCT116 cells. However, these extracts were relatively less toxic to non-cancer Vero cells. HPLC analysis demonstrated that the powder mix extracts contained seven identified phenolic acids namely gallic, p-hydroxybenzoic, vanillic, syringic, p-coumaric, ferulic and sinapinic acids, where p-coumaric acid was detected in the highest concentration followed by ferulic acid. CONCLUSION: Overall, the results of this study suggest the powdered formula of H. cordata fermented broth and P. emblica fruit as an alternative medicine for cancer prevention and treatment.
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spelling pubmed-58960452018-04-12 Antiproliferative activities and phenolic acid content of water and ethanolic extracts of the powdered formula of Houttuynia cordata Thunb. fermented broth and Phyllanthus emblica Linn. fruit Kumnerdkhonkaen, Piyawan Saenglee, Somprasong Asgar, Md. Ali Senawong, Gulsiri Khongsukwiwat, Kanoknan Senawong, Thanaset BMC Complement Altern Med Research Article BACKGROUND: Houttuynia cordata Thunb. and Phyllanthus emblica Linn. are native plants with medicinal and nutritive significance in Asia. The present study was aimed at evaluating antiproliferative effects on human cancer cell lines and identifying the phenolic acid composition of water and ethanolic extracts of the powdered formula of H. cordata fermented broth and P. emblica fruit. METHODS: Anticancer activity of the extracts was evaluated against HeLa, HT29, HCT116, MCF7 and Jurkat cells using an MTT assay and flow cytometric analysis of apoptosis induction and cell cycle arrest. Reverse phase HPLC was exploited for identification and quantification of some phenolic acids. RESULTS: MTT assay showed that both water and ethanolic extracts significantly decreased the viability of cancer cells in a dose- and time-dependent fashion. Based on the IC(50) values, ethanolic extract (IC(50) values = 0.12–0.65 mg/mL) was more cytotoxic than water extract (IC(50) values = 0.22–0.85 mg/mL) and Jurkat cells were the most sensitive to both extracts (IC(50) values = 0.12–0.69 mg/mL). The underlying mechanism for antiproliferative activity was apoptosis induction, especially in HT29, HCT116, MCF7 and Jurkat cells. HT29 cells were the most sensitive to extract-induced apoptosis. Ethanolic extract was more effective at inducing apoptosis than water extract. Moreover, cell cycle arrest was found to be another mechanism behind growth inhibition in Jurkat and HCT116 cells. However, these extracts were relatively less toxic to non-cancer Vero cells. HPLC analysis demonstrated that the powder mix extracts contained seven identified phenolic acids namely gallic, p-hydroxybenzoic, vanillic, syringic, p-coumaric, ferulic and sinapinic acids, where p-coumaric acid was detected in the highest concentration followed by ferulic acid. CONCLUSION: Overall, the results of this study suggest the powdered formula of H. cordata fermented broth and P. emblica fruit as an alternative medicine for cancer prevention and treatment. BioMed Central 2018-04-11 /pmc/articles/PMC5896045/ /pubmed/29642867 http://dx.doi.org/10.1186/s12906-018-2185-x Text en © The Author(s). 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Kumnerdkhonkaen, Piyawan
Saenglee, Somprasong
Asgar, Md. Ali
Senawong, Gulsiri
Khongsukwiwat, Kanoknan
Senawong, Thanaset
Antiproliferative activities and phenolic acid content of water and ethanolic extracts of the powdered formula of Houttuynia cordata Thunb. fermented broth and Phyllanthus emblica Linn. fruit
title Antiproliferative activities and phenolic acid content of water and ethanolic extracts of the powdered formula of Houttuynia cordata Thunb. fermented broth and Phyllanthus emblica Linn. fruit
title_full Antiproliferative activities and phenolic acid content of water and ethanolic extracts of the powdered formula of Houttuynia cordata Thunb. fermented broth and Phyllanthus emblica Linn. fruit
title_fullStr Antiproliferative activities and phenolic acid content of water and ethanolic extracts of the powdered formula of Houttuynia cordata Thunb. fermented broth and Phyllanthus emblica Linn. fruit
title_full_unstemmed Antiproliferative activities and phenolic acid content of water and ethanolic extracts of the powdered formula of Houttuynia cordata Thunb. fermented broth and Phyllanthus emblica Linn. fruit
title_short Antiproliferative activities and phenolic acid content of water and ethanolic extracts of the powdered formula of Houttuynia cordata Thunb. fermented broth and Phyllanthus emblica Linn. fruit
title_sort antiproliferative activities and phenolic acid content of water and ethanolic extracts of the powdered formula of houttuynia cordata thunb. fermented broth and phyllanthus emblica linn. fruit
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5896045/
https://www.ncbi.nlm.nih.gov/pubmed/29642867
http://dx.doi.org/10.1186/s12906-018-2185-x
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