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Comparative transcriptome analysis of hypothalamus-regulated feed intake induced by exogenous visfatin in chicks

BACKGROUND: The intracerebroventricular injection of visfatin increases feed intake. However, little is known about the molecular mechanism in chicks. This study was conducted to assess the effect of visfatin on the feeding behavior of chicks and the associated molecular mechanism. RESULTS: In respo...

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Autores principales: Li, Zhuanjian, Liu, Xuelian, Zhang, Panpan, Han, Ruili, Sun, Guirong, Jiang, Ruirui, Wang, Yanbin, Liu, Xiaojun, Li, Wenya, Kang, Xiangtao, Tian, Yadong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5896085/
https://www.ncbi.nlm.nih.gov/pubmed/29642854
http://dx.doi.org/10.1186/s12864-018-4644-7
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author Li, Zhuanjian
Liu, Xuelian
Zhang, Panpan
Han, Ruili
Sun, Guirong
Jiang, Ruirui
Wang, Yanbin
Liu, Xiaojun
Li, Wenya
Kang, Xiangtao
Tian, Yadong
author_facet Li, Zhuanjian
Liu, Xuelian
Zhang, Panpan
Han, Ruili
Sun, Guirong
Jiang, Ruirui
Wang, Yanbin
Liu, Xiaojun
Li, Wenya
Kang, Xiangtao
Tian, Yadong
author_sort Li, Zhuanjian
collection PubMed
description BACKGROUND: The intracerebroventricular injection of visfatin increases feed intake. However, little is known about the molecular mechanism in chicks. This study was conducted to assess the effect of visfatin on the feeding behavior of chicks and the associated molecular mechanism. RESULTS: In response to the intraventricular injection of 40 ng and 400 ng visfatin, feed intake in chicks was significantly increased, and the concentrations of glucose, insulin, TG, HDL and LDL were significantly altered. Using RNA-seq, we identified DEGs in the chick hypothalamus at 60 min after injection with various doses of visfatin. In total, 325, 85 and 519 DEGs were identified in the treated chick hypothalamus in the LT vs C, HT vs C and LT vs HT comparisons, respectively. The changes in the expression profiles of DEGs, GO functional categories, KEGG pathways, and PPI networks by visfatin-mediated regulation of feed intake were analyzed. The DEGs were grouped into 8 clusters based on their expression patterns via K-mean clustering; there were 14 appetite-related DEGs enriched in the hormone activity GO term. The neuroactive ligand-receptor interaction pathway was the key pathway affected by visfatin. The PPI analysis of DEGs showed that POMC was a hub gene that interacted with the maximum number of nodes and ingestion-related pathways, including POMC, CRH, AgRP, NPY, TRH, VIP, NPYL, CGA and TSHB. CONCLUSION: These common DEGs were enriched in the hormone activity GO term and the neuroactive ligand-receptor interaction pathway. Therefore, visfatin causes hyperphagia via the POMC/CRH and NPY/AgRP signaling pathways. These results provide valuable information about the molecular mechanisms of the regulation of food intake by visfatin. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-018-4644-7) contains supplementary material, which is available to authorized users.
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spelling pubmed-58960852018-04-20 Comparative transcriptome analysis of hypothalamus-regulated feed intake induced by exogenous visfatin in chicks Li, Zhuanjian Liu, Xuelian Zhang, Panpan Han, Ruili Sun, Guirong Jiang, Ruirui Wang, Yanbin Liu, Xiaojun Li, Wenya Kang, Xiangtao Tian, Yadong BMC Genomics Research Article BACKGROUND: The intracerebroventricular injection of visfatin increases feed intake. However, little is known about the molecular mechanism in chicks. This study was conducted to assess the effect of visfatin on the feeding behavior of chicks and the associated molecular mechanism. RESULTS: In response to the intraventricular injection of 40 ng and 400 ng visfatin, feed intake in chicks was significantly increased, and the concentrations of glucose, insulin, TG, HDL and LDL were significantly altered. Using RNA-seq, we identified DEGs in the chick hypothalamus at 60 min after injection with various doses of visfatin. In total, 325, 85 and 519 DEGs were identified in the treated chick hypothalamus in the LT vs C, HT vs C and LT vs HT comparisons, respectively. The changes in the expression profiles of DEGs, GO functional categories, KEGG pathways, and PPI networks by visfatin-mediated regulation of feed intake were analyzed. The DEGs were grouped into 8 clusters based on their expression patterns via K-mean clustering; there were 14 appetite-related DEGs enriched in the hormone activity GO term. The neuroactive ligand-receptor interaction pathway was the key pathway affected by visfatin. The PPI analysis of DEGs showed that POMC was a hub gene that interacted with the maximum number of nodes and ingestion-related pathways, including POMC, CRH, AgRP, NPY, TRH, VIP, NPYL, CGA and TSHB. CONCLUSION: These common DEGs were enriched in the hormone activity GO term and the neuroactive ligand-receptor interaction pathway. Therefore, visfatin causes hyperphagia via the POMC/CRH and NPY/AgRP signaling pathways. These results provide valuable information about the molecular mechanisms of the regulation of food intake by visfatin. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-018-4644-7) contains supplementary material, which is available to authorized users. BioMed Central 2018-04-11 /pmc/articles/PMC5896085/ /pubmed/29642854 http://dx.doi.org/10.1186/s12864-018-4644-7 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Li, Zhuanjian
Liu, Xuelian
Zhang, Panpan
Han, Ruili
Sun, Guirong
Jiang, Ruirui
Wang, Yanbin
Liu, Xiaojun
Li, Wenya
Kang, Xiangtao
Tian, Yadong
Comparative transcriptome analysis of hypothalamus-regulated feed intake induced by exogenous visfatin in chicks
title Comparative transcriptome analysis of hypothalamus-regulated feed intake induced by exogenous visfatin in chicks
title_full Comparative transcriptome analysis of hypothalamus-regulated feed intake induced by exogenous visfatin in chicks
title_fullStr Comparative transcriptome analysis of hypothalamus-regulated feed intake induced by exogenous visfatin in chicks
title_full_unstemmed Comparative transcriptome analysis of hypothalamus-regulated feed intake induced by exogenous visfatin in chicks
title_short Comparative transcriptome analysis of hypothalamus-regulated feed intake induced by exogenous visfatin in chicks
title_sort comparative transcriptome analysis of hypothalamus-regulated feed intake induced by exogenous visfatin in chicks
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5896085/
https://www.ncbi.nlm.nih.gov/pubmed/29642854
http://dx.doi.org/10.1186/s12864-018-4644-7
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