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Enhanced Suppressive Activity of Regulatory T Cells in the Microenvironment of Malignant Pleural Effusions

Cancer metastatic spread to serous cavity causes malignant pleural effusions (MPEs), indicating dismal prognosis. Tumor microenvironment can implement suppressive activity on host immune responses. Thus, we investigated the prevalence of Tregs and the relationship between them and TGF-β and IL-10 co...

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Autores principales: Budna, Joanna, Kaczmarek, Mariusz, Kolecka-Bednarczyk, Agata, Spychalski, Łukasz, Zawierucha, Piotr, Goździk-Spychalska, Joanna, Nowicki, Michał, Batura-Gabryel, Halina, Sikora, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5896249/
https://www.ncbi.nlm.nih.gov/pubmed/29785404
http://dx.doi.org/10.1155/2018/9876014
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author Budna, Joanna
Kaczmarek, Mariusz
Kolecka-Bednarczyk, Agata
Spychalski, Łukasz
Zawierucha, Piotr
Goździk-Spychalska, Joanna
Nowicki, Michał
Batura-Gabryel, Halina
Sikora, Jan
author_facet Budna, Joanna
Kaczmarek, Mariusz
Kolecka-Bednarczyk, Agata
Spychalski, Łukasz
Zawierucha, Piotr
Goździk-Spychalska, Joanna
Nowicki, Michał
Batura-Gabryel, Halina
Sikora, Jan
author_sort Budna, Joanna
collection PubMed
description Cancer metastatic spread to serous cavity causes malignant pleural effusions (MPEs), indicating dismal prognosis. Tumor microenvironment can implement suppressive activity on host immune responses. Thus, we investigated the prevalence of Tregs and the relationship between them and TGF-β and IL-10 concentrations and measured expression of FOXP3, CTLA-4, CD28, and GITR genes, as well as protein expression of selected genes in benign effusions and MPEs. The percentage of Tregs was determined by means of multicolor flow cytometry system. TGF-β and IL-10 concentrations were measured using human TGF-β1 and IL-10 ELISA kit. Relative mRNA expression of studied genes was analyzed by real-time PCR. The frequency of Tregs was significantly higher in MPEs compared to benign effusions; however, the level of TGF-β and IL-10 in analyzed groups was comparable, and no correlation between concentrations of TGF-β and IL-10 and percentage of Tregs was observed. Relative mRNA expression of all the genes was higher in CD4(+)CD25(+) compared to CD4(+)CD25(−) cells. In CD4(+)CD25(+) cells from MPEs, relative mRNA expression of FOXP3, CTLA-4, and CD28 genes was significantly higher than in benign effusions; however, the level of CD4(+)CD25(+)CTLA-4(+) cells in analyzed groups showed no significant differences. We found numerous genes correlations in an entire CD4(+)CD25(+) cell subset and CD4(+)CD25(+) cells from MPEs. Enhanced suppressive activity of Tregs is observed in the microenvironment of MPEs. Understanding of relations between cellular and cytokine immunosuppressive factors in tumor microenvironment may determine success of anticancer response.
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spelling pubmed-58962492018-05-21 Enhanced Suppressive Activity of Regulatory T Cells in the Microenvironment of Malignant Pleural Effusions Budna, Joanna Kaczmarek, Mariusz Kolecka-Bednarczyk, Agata Spychalski, Łukasz Zawierucha, Piotr Goździk-Spychalska, Joanna Nowicki, Michał Batura-Gabryel, Halina Sikora, Jan J Immunol Res Research Article Cancer metastatic spread to serous cavity causes malignant pleural effusions (MPEs), indicating dismal prognosis. Tumor microenvironment can implement suppressive activity on host immune responses. Thus, we investigated the prevalence of Tregs and the relationship between them and TGF-β and IL-10 concentrations and measured expression of FOXP3, CTLA-4, CD28, and GITR genes, as well as protein expression of selected genes in benign effusions and MPEs. The percentage of Tregs was determined by means of multicolor flow cytometry system. TGF-β and IL-10 concentrations were measured using human TGF-β1 and IL-10 ELISA kit. Relative mRNA expression of studied genes was analyzed by real-time PCR. The frequency of Tregs was significantly higher in MPEs compared to benign effusions; however, the level of TGF-β and IL-10 in analyzed groups was comparable, and no correlation between concentrations of TGF-β and IL-10 and percentage of Tregs was observed. Relative mRNA expression of all the genes was higher in CD4(+)CD25(+) compared to CD4(+)CD25(−) cells. In CD4(+)CD25(+) cells from MPEs, relative mRNA expression of FOXP3, CTLA-4, and CD28 genes was significantly higher than in benign effusions; however, the level of CD4(+)CD25(+)CTLA-4(+) cells in analyzed groups showed no significant differences. We found numerous genes correlations in an entire CD4(+)CD25(+) cell subset and CD4(+)CD25(+) cells from MPEs. Enhanced suppressive activity of Tregs is observed in the microenvironment of MPEs. Understanding of relations between cellular and cytokine immunosuppressive factors in tumor microenvironment may determine success of anticancer response. Hindawi 2018-03-26 /pmc/articles/PMC5896249/ /pubmed/29785404 http://dx.doi.org/10.1155/2018/9876014 Text en Copyright © 2018 Joanna Budna et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Budna, Joanna
Kaczmarek, Mariusz
Kolecka-Bednarczyk, Agata
Spychalski, Łukasz
Zawierucha, Piotr
Goździk-Spychalska, Joanna
Nowicki, Michał
Batura-Gabryel, Halina
Sikora, Jan
Enhanced Suppressive Activity of Regulatory T Cells in the Microenvironment of Malignant Pleural Effusions
title Enhanced Suppressive Activity of Regulatory T Cells in the Microenvironment of Malignant Pleural Effusions
title_full Enhanced Suppressive Activity of Regulatory T Cells in the Microenvironment of Malignant Pleural Effusions
title_fullStr Enhanced Suppressive Activity of Regulatory T Cells in the Microenvironment of Malignant Pleural Effusions
title_full_unstemmed Enhanced Suppressive Activity of Regulatory T Cells in the Microenvironment of Malignant Pleural Effusions
title_short Enhanced Suppressive Activity of Regulatory T Cells in the Microenvironment of Malignant Pleural Effusions
title_sort enhanced suppressive activity of regulatory t cells in the microenvironment of malignant pleural effusions
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5896249/
https://www.ncbi.nlm.nih.gov/pubmed/29785404
http://dx.doi.org/10.1155/2018/9876014
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