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The Interaction between Bmal1 and Per2 in Mouse BMSC Osteogenic Differentiation

The circadian clock is a system that controls endogenous time of organisms, and it regulates the physiology and behavior of bodies. The transcription factors Brain and Muscle ARNT-like Protein 1 (BMAL1) and Period2 (Per2) are components of the circadian clock, and they play vital roles in circadian...

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Detalles Bibliográficos
Autores principales: Zhuo, Haiya, Wang, Yuhong, Zhao, Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5896276/
https://www.ncbi.nlm.nih.gov/pubmed/29765408
http://dx.doi.org/10.1155/2018/3407821
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author Zhuo, Haiya
Wang, Yuhong
Zhao, Qing
author_facet Zhuo, Haiya
Wang, Yuhong
Zhao, Qing
author_sort Zhuo, Haiya
collection PubMed
description The circadian clock is a system that controls endogenous time of organisms, and it regulates the physiology and behavior of bodies. The transcription factors Brain and Muscle ARNT-like Protein 1 (BMAL1) and Period2 (Per2) are components of the circadian clock, and they play vital roles in circadian clock function. Both Bmal1−/− mice and Per2−/− mice display obvious bone volume changes. In this study, we inhibited the expression of Bmal1 in bone marrow-derived mesenchymal stem cells (BMSCs) using a lentiviral vector harboring RNAi sequences, which increased the osteogenic differentiation capability of BMSCs. We also suppressed Per2 gene expression using an adenovirus vector harboring RNAi sequences, and similarly, the osteogenic differentiation ability of BMSCs was enhanced. Furthermore, when both Bmal1 and Per2 gene expression was suppressed in BMSCs by lentiviral and adenoviral interference, the osteogenic differentiation capability was stronger than that in BMSCs following single-gene inhibition. Our data support that both Bmal1 and Per2 play negative roles in BMSC osteogenic differentiation and that Bmal1 and Per2 have a synergistic effect on the osteogenic differentiation of BMSCs.
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spelling pubmed-58962762018-05-14 The Interaction between Bmal1 and Per2 in Mouse BMSC Osteogenic Differentiation Zhuo, Haiya Wang, Yuhong Zhao, Qing Stem Cells Int Research Article The circadian clock is a system that controls endogenous time of organisms, and it regulates the physiology and behavior of bodies. The transcription factors Brain and Muscle ARNT-like Protein 1 (BMAL1) and Period2 (Per2) are components of the circadian clock, and they play vital roles in circadian clock function. Both Bmal1−/− mice and Per2−/− mice display obvious bone volume changes. In this study, we inhibited the expression of Bmal1 in bone marrow-derived mesenchymal stem cells (BMSCs) using a lentiviral vector harboring RNAi sequences, which increased the osteogenic differentiation capability of BMSCs. We also suppressed Per2 gene expression using an adenovirus vector harboring RNAi sequences, and similarly, the osteogenic differentiation ability of BMSCs was enhanced. Furthermore, when both Bmal1 and Per2 gene expression was suppressed in BMSCs by lentiviral and adenoviral interference, the osteogenic differentiation capability was stronger than that in BMSCs following single-gene inhibition. Our data support that both Bmal1 and Per2 play negative roles in BMSC osteogenic differentiation and that Bmal1 and Per2 have a synergistic effect on the osteogenic differentiation of BMSCs. Hindawi 2018-03-29 /pmc/articles/PMC5896276/ /pubmed/29765408 http://dx.doi.org/10.1155/2018/3407821 Text en Copyright © 2018 Haiya Zhuo et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhuo, Haiya
Wang, Yuhong
Zhao, Qing
The Interaction between Bmal1 and Per2 in Mouse BMSC Osteogenic Differentiation
title The Interaction between Bmal1 and Per2 in Mouse BMSC Osteogenic Differentiation
title_full The Interaction between Bmal1 and Per2 in Mouse BMSC Osteogenic Differentiation
title_fullStr The Interaction between Bmal1 and Per2 in Mouse BMSC Osteogenic Differentiation
title_full_unstemmed The Interaction between Bmal1 and Per2 in Mouse BMSC Osteogenic Differentiation
title_short The Interaction between Bmal1 and Per2 in Mouse BMSC Osteogenic Differentiation
title_sort interaction between bmal1 and per2 in mouse bmsc osteogenic differentiation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5896276/
https://www.ncbi.nlm.nih.gov/pubmed/29765408
http://dx.doi.org/10.1155/2018/3407821
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