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Cellular interaction of a layer-by-layer based drug delivery system depending on material properties and cell types

BACKGROUND: Drug delivery systems (DDS) and their interaction with cells are a controversial topic in the development of therapeutic concepts and approaches. On one hand, DDS are very useful for protected and targeted transport of defined dosages of active agents. On the other hand, their physicoche...

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Autores principales: Brueckner, Mandy, Jankuhn, Steffen, Jülke, Eva-Maria, Reibetanz, Uta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5896659/
https://www.ncbi.nlm.nih.gov/pubmed/29670351
http://dx.doi.org/10.2147/IJN.S153701
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author Brueckner, Mandy
Jankuhn, Steffen
Jülke, Eva-Maria
Reibetanz, Uta
author_facet Brueckner, Mandy
Jankuhn, Steffen
Jülke, Eva-Maria
Reibetanz, Uta
author_sort Brueckner, Mandy
collection PubMed
description BACKGROUND: Drug delivery systems (DDS) and their interaction with cells are a controversial topic in the development of therapeutic concepts and approaches. On one hand, DDS are very useful for protected and targeted transport of defined dosages of active agents. On the other hand, their physicochemical properties such as material, size, shape, charge, or stiffness have a huge impact on cellular uptake and intracellular processing. Additionally, even identical DDS can undergo a completely diverse interaction with different cell types. However, quite often in in vitro DDS/cell interaction experiments, those aspects are not considered and DDS and cells are randomly chosen. METHODS AND RESULTS: Hence, our investigations provide an insight into layer-by-layer designed microcarriers with modifications of only some of the most important parameters (surface charge, stiffness, and applied microcarrier/cell ratio) and their influence on cellular uptake and viability. We also considered the interaction of these differently equipped DDS with several cell types and investigated professional phagocytes (neutrophil granulocytes; macrophages) as well as non-professional phagocytes (epithelial cells) under comparable conditions. We found that even small modifications such as layer-by-layer (LbL)-microcarriers with positive or negative surface charge, or LbL-microcarriers with solid core or as hollow capsules but equipped with the same surface properties, show significant differences in interaction and viability, and several cell types react very differently to the offered DDS. CONCLUSION: As a consequence, the properties of the DDS have to be carefully chosen with respect to the addressed cell type with the aim to efficiently transport a desired agent.
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spelling pubmed-58966592018-04-18 Cellular interaction of a layer-by-layer based drug delivery system depending on material properties and cell types Brueckner, Mandy Jankuhn, Steffen Jülke, Eva-Maria Reibetanz, Uta Int J Nanomedicine Original Research BACKGROUND: Drug delivery systems (DDS) and their interaction with cells are a controversial topic in the development of therapeutic concepts and approaches. On one hand, DDS are very useful for protected and targeted transport of defined dosages of active agents. On the other hand, their physicochemical properties such as material, size, shape, charge, or stiffness have a huge impact on cellular uptake and intracellular processing. Additionally, even identical DDS can undergo a completely diverse interaction with different cell types. However, quite often in in vitro DDS/cell interaction experiments, those aspects are not considered and DDS and cells are randomly chosen. METHODS AND RESULTS: Hence, our investigations provide an insight into layer-by-layer designed microcarriers with modifications of only some of the most important parameters (surface charge, stiffness, and applied microcarrier/cell ratio) and their influence on cellular uptake and viability. We also considered the interaction of these differently equipped DDS with several cell types and investigated professional phagocytes (neutrophil granulocytes; macrophages) as well as non-professional phagocytes (epithelial cells) under comparable conditions. We found that even small modifications such as layer-by-layer (LbL)-microcarriers with positive or negative surface charge, or LbL-microcarriers with solid core or as hollow capsules but equipped with the same surface properties, show significant differences in interaction and viability, and several cell types react very differently to the offered DDS. CONCLUSION: As a consequence, the properties of the DDS have to be carefully chosen with respect to the addressed cell type with the aim to efficiently transport a desired agent. Dove Medical Press 2018-04-05 /pmc/articles/PMC5896659/ /pubmed/29670351 http://dx.doi.org/10.2147/IJN.S153701 Text en © 2018 Brueckner et al. This work is published by Dove Medical Press Limited, and licensed under a Creative Commons Attribution License The full terms of the License are available at http://creativecommons.org/licenses/by/4.0/. The license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Original Research
Brueckner, Mandy
Jankuhn, Steffen
Jülke, Eva-Maria
Reibetanz, Uta
Cellular interaction of a layer-by-layer based drug delivery system depending on material properties and cell types
title Cellular interaction of a layer-by-layer based drug delivery system depending on material properties and cell types
title_full Cellular interaction of a layer-by-layer based drug delivery system depending on material properties and cell types
title_fullStr Cellular interaction of a layer-by-layer based drug delivery system depending on material properties and cell types
title_full_unstemmed Cellular interaction of a layer-by-layer based drug delivery system depending on material properties and cell types
title_short Cellular interaction of a layer-by-layer based drug delivery system depending on material properties and cell types
title_sort cellular interaction of a layer-by-layer based drug delivery system depending on material properties and cell types
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5896659/
https://www.ncbi.nlm.nih.gov/pubmed/29670351
http://dx.doi.org/10.2147/IJN.S153701
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