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Membrane detachment is not essential for COG complex function

The conserved oligomeric Golgi (COG) complex is a vesicle tether of the “complexes associated with tethering containing helical rods” family, which functions on the cytoplasmic side of Golgi. It is currently unknown whether COG function, or function of any multisubunit vesicular tether, depends on c...

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Autores principales: Climer, Leslie K., Pokrovskaya, Irina D., Blackburn, Jessica B., Lupashin, Vladimir V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5896934/
https://www.ncbi.nlm.nih.gov/pubmed/29467253
http://dx.doi.org/10.1091/mbc.E17-11-0694
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author Climer, Leslie K.
Pokrovskaya, Irina D.
Blackburn, Jessica B.
Lupashin, Vladimir V.
author_facet Climer, Leslie K.
Pokrovskaya, Irina D.
Blackburn, Jessica B.
Lupashin, Vladimir V.
author_sort Climer, Leslie K.
collection PubMed
description The conserved oligomeric Golgi (COG) complex is a vesicle tether of the “complexes associated with tethering containing helical rods” family, which functions on the cytoplasmic side of Golgi. It is currently unknown whether COG function, or function of any multisubunit vesicular tether, depends on cycling between the membrane and cytosol. Therefore, we permanently anchored key subunits of COG subcomplexes (COG4, COG7, and COG8) to Golgi membranes using transmembrane protein TMEM115 (TMEM-COG). All TMEM-COG subunits tested were Golgi localized, integrated into the COG complex, and stabilized membrane association of endogenous subunits. Interestingly, TMEM-COG4 and TMEM-COG7 equally rescued COG function in organization of Golgi markers, glycosylation, and abundance of COG-sensitive proteins. In contrast, TMEM-COG8 was not as effective, indicating that N-terminal attachment of COG8 interfered with overall COG structure and function, and none of the TMEM-COG subunits rescued the abnormal Golgi architecture caused by COG knockout. Collectively, these data indicate that both subcomplexes of the COG complex can perform most of COG function when permanently attached to membranes and that the cytosolic pool of COG is not completely essential to COG function.
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spelling pubmed-58969342018-06-30 Membrane detachment is not essential for COG complex function Climer, Leslie K. Pokrovskaya, Irina D. Blackburn, Jessica B. Lupashin, Vladimir V. Mol Biol Cell Articles The conserved oligomeric Golgi (COG) complex is a vesicle tether of the “complexes associated with tethering containing helical rods” family, which functions on the cytoplasmic side of Golgi. It is currently unknown whether COG function, or function of any multisubunit vesicular tether, depends on cycling between the membrane and cytosol. Therefore, we permanently anchored key subunits of COG subcomplexes (COG4, COG7, and COG8) to Golgi membranes using transmembrane protein TMEM115 (TMEM-COG). All TMEM-COG subunits tested were Golgi localized, integrated into the COG complex, and stabilized membrane association of endogenous subunits. Interestingly, TMEM-COG4 and TMEM-COG7 equally rescued COG function in organization of Golgi markers, glycosylation, and abundance of COG-sensitive proteins. In contrast, TMEM-COG8 was not as effective, indicating that N-terminal attachment of COG8 interfered with overall COG structure and function, and none of the TMEM-COG subunits rescued the abnormal Golgi architecture caused by COG knockout. Collectively, these data indicate that both subcomplexes of the COG complex can perform most of COG function when permanently attached to membranes and that the cytosolic pool of COG is not completely essential to COG function. The American Society for Cell Biology 2018-04-15 /pmc/articles/PMC5896934/ /pubmed/29467253 http://dx.doi.org/10.1091/mbc.E17-11-0694 Text en © 2018 Climer et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0/ This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
spellingShingle Articles
Climer, Leslie K.
Pokrovskaya, Irina D.
Blackburn, Jessica B.
Lupashin, Vladimir V.
Membrane detachment is not essential for COG complex function
title Membrane detachment is not essential for COG complex function
title_full Membrane detachment is not essential for COG complex function
title_fullStr Membrane detachment is not essential for COG complex function
title_full_unstemmed Membrane detachment is not essential for COG complex function
title_short Membrane detachment is not essential for COG complex function
title_sort membrane detachment is not essential for cog complex function
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5896934/
https://www.ncbi.nlm.nih.gov/pubmed/29467253
http://dx.doi.org/10.1091/mbc.E17-11-0694
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