Cargando…
Ultra-Rapid Crystallization of L-alanine Using Monomode Microwaves, Indium Tin Oxide and Metal-Assisted and Microwave-Accelerated Evaporative Crystallization
The use of indium tin oxide (ITO) and focused monomode microwave heating for the ultra-rapid crystallization of L-alanine (a model amino acid) is reported. Commercially available ITO dots (< 5 mm) attached to blank poly(methyl)methacrylate (PMMA, 5 cm in diameter with 21-well silicon isolators: r...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5897105/ https://www.ncbi.nlm.nih.gov/pubmed/29657884 http://dx.doi.org/10.5101/nbe.v9i2.p112-123 |
_version_ | 1783313915179958272 |
---|---|
author | Lansiquot, Carisse Boone-Kukoyi, Zainab Shortt, Raquel Thompson, Nishone Ajifa, Hillary Kioko, Bridgit Constance, Edward Ned Clement, Travis Ozturk, Birol Aslan, Kadir |
author_facet | Lansiquot, Carisse Boone-Kukoyi, Zainab Shortt, Raquel Thompson, Nishone Ajifa, Hillary Kioko, Bridgit Constance, Edward Ned Clement, Travis Ozturk, Birol Aslan, Kadir |
author_sort | Lansiquot, Carisse |
collection | PubMed |
description | The use of indium tin oxide (ITO) and focused monomode microwave heating for the ultra-rapid crystallization of L-alanine (a model amino acid) is reported. Commercially available ITO dots (< 5 mm) attached to blank poly(methyl)methacrylate (PMMA, 5 cm in diameter with 21-well silicon isolators: referred to as the iCrystal plates) were found to withstand prolonged microwave heating during crystallization experiments. Crystallization of L-alanine was performed at room temperature (a control experiment), with the use of two microwave sources: a 2.45 GHz conventional microwave (900 W, power level 1, a control experiment) and 8 GHz (20 W) solid state, monomode microwave source with an applicator tip that focuses the microwave field to a 5-mm cavity. Initial appearance of L-alanine crystals and on iCrystal plates with ITO dots took 47 ± 2.9 min, 12 ± 7.6 min and 1.5 ± 0.5 min at room temperature, using a conventional microwave and focused monomode microwave heating, respectively. Complete evaporation of the solvent using the focused microwaves was achieved in 3.2 ± 0.5 min, which is ~52-fold and ~172-fold faster than that observed at room temperature and using conventional microwave heating, respectively. The size and number of L-alanine crystals was dependent on the type of the 21-well iCrystal plates and the microwave heating method: 33 crystals of 585 ± 137 μm in size at room temperature > 37 crystals of 542 ± 100 μm in size with conventional microwave heating > 331 crystals of 311 ± 190 μm in size with focused monomode microwave. FTIR, optical microscopy and powder X-ray diffraction analysis showed that the chemical composition and crystallinity of the L-alanine crystals did not change when exposed to microwave heating and ITO surfaces. In addition, theoretical simulations for the binding of L-alanine molecules to ITO and other metals showed the predicted nature of hydrogen bonds formed between L-alanine and these surfaces. |
format | Online Article Text |
id | pubmed-5897105 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
record_format | MEDLINE/PubMed |
spelling | pubmed-58971052018-04-12 Ultra-Rapid Crystallization of L-alanine Using Monomode Microwaves, Indium Tin Oxide and Metal-Assisted and Microwave-Accelerated Evaporative Crystallization Lansiquot, Carisse Boone-Kukoyi, Zainab Shortt, Raquel Thompson, Nishone Ajifa, Hillary Kioko, Bridgit Constance, Edward Ned Clement, Travis Ozturk, Birol Aslan, Kadir Nano Biomed Eng Article The use of indium tin oxide (ITO) and focused monomode microwave heating for the ultra-rapid crystallization of L-alanine (a model amino acid) is reported. Commercially available ITO dots (< 5 mm) attached to blank poly(methyl)methacrylate (PMMA, 5 cm in diameter with 21-well silicon isolators: referred to as the iCrystal plates) were found to withstand prolonged microwave heating during crystallization experiments. Crystallization of L-alanine was performed at room temperature (a control experiment), with the use of two microwave sources: a 2.45 GHz conventional microwave (900 W, power level 1, a control experiment) and 8 GHz (20 W) solid state, monomode microwave source with an applicator tip that focuses the microwave field to a 5-mm cavity. Initial appearance of L-alanine crystals and on iCrystal plates with ITO dots took 47 ± 2.9 min, 12 ± 7.6 min and 1.5 ± 0.5 min at room temperature, using a conventional microwave and focused monomode microwave heating, respectively. Complete evaporation of the solvent using the focused microwaves was achieved in 3.2 ± 0.5 min, which is ~52-fold and ~172-fold faster than that observed at room temperature and using conventional microwave heating, respectively. The size and number of L-alanine crystals was dependent on the type of the 21-well iCrystal plates and the microwave heating method: 33 crystals of 585 ± 137 μm in size at room temperature > 37 crystals of 542 ± 100 μm in size with conventional microwave heating > 331 crystals of 311 ± 190 μm in size with focused monomode microwave. FTIR, optical microscopy and powder X-ray diffraction analysis showed that the chemical composition and crystallinity of the L-alanine crystals did not change when exposed to microwave heating and ITO surfaces. In addition, theoretical simulations for the binding of L-alanine molecules to ITO and other metals showed the predicted nature of hydrogen bonds formed between L-alanine and these surfaces. 2017-05-27 2017 /pmc/articles/PMC5897105/ /pubmed/29657884 http://dx.doi.org/10.5101/nbe.v9i2.p112-123 Text en http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Article Lansiquot, Carisse Boone-Kukoyi, Zainab Shortt, Raquel Thompson, Nishone Ajifa, Hillary Kioko, Bridgit Constance, Edward Ned Clement, Travis Ozturk, Birol Aslan, Kadir Ultra-Rapid Crystallization of L-alanine Using Monomode Microwaves, Indium Tin Oxide and Metal-Assisted and Microwave-Accelerated Evaporative Crystallization |
title | Ultra-Rapid Crystallization of L-alanine Using Monomode Microwaves, Indium Tin Oxide and Metal-Assisted and Microwave-Accelerated Evaporative Crystallization |
title_full | Ultra-Rapid Crystallization of L-alanine Using Monomode Microwaves, Indium Tin Oxide and Metal-Assisted and Microwave-Accelerated Evaporative Crystallization |
title_fullStr | Ultra-Rapid Crystallization of L-alanine Using Monomode Microwaves, Indium Tin Oxide and Metal-Assisted and Microwave-Accelerated Evaporative Crystallization |
title_full_unstemmed | Ultra-Rapid Crystallization of L-alanine Using Monomode Microwaves, Indium Tin Oxide and Metal-Assisted and Microwave-Accelerated Evaporative Crystallization |
title_short | Ultra-Rapid Crystallization of L-alanine Using Monomode Microwaves, Indium Tin Oxide and Metal-Assisted and Microwave-Accelerated Evaporative Crystallization |
title_sort | ultra-rapid crystallization of l-alanine using monomode microwaves, indium tin oxide and metal-assisted and microwave-accelerated evaporative crystallization |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5897105/ https://www.ncbi.nlm.nih.gov/pubmed/29657884 http://dx.doi.org/10.5101/nbe.v9i2.p112-123 |
work_keys_str_mv | AT lansiquotcarisse ultrarapidcrystallizationoflalanineusingmonomodemicrowavesindiumtinoxideandmetalassistedandmicrowaveacceleratedevaporativecrystallization AT boonekukoyizainab ultrarapidcrystallizationoflalanineusingmonomodemicrowavesindiumtinoxideandmetalassistedandmicrowaveacceleratedevaporativecrystallization AT shorttraquel ultrarapidcrystallizationoflalanineusingmonomodemicrowavesindiumtinoxideandmetalassistedandmicrowaveacceleratedevaporativecrystallization AT thompsonnishone ultrarapidcrystallizationoflalanineusingmonomodemicrowavesindiumtinoxideandmetalassistedandmicrowaveacceleratedevaporativecrystallization AT ajifahillary ultrarapidcrystallizationoflalanineusingmonomodemicrowavesindiumtinoxideandmetalassistedandmicrowaveacceleratedevaporativecrystallization AT kiokobridgit ultrarapidcrystallizationoflalanineusingmonomodemicrowavesindiumtinoxideandmetalassistedandmicrowaveacceleratedevaporativecrystallization AT constanceedwardned ultrarapidcrystallizationoflalanineusingmonomodemicrowavesindiumtinoxideandmetalassistedandmicrowaveacceleratedevaporativecrystallization AT clementtravis ultrarapidcrystallizationoflalanineusingmonomodemicrowavesindiumtinoxideandmetalassistedandmicrowaveacceleratedevaporativecrystallization AT ozturkbirol ultrarapidcrystallizationoflalanineusingmonomodemicrowavesindiumtinoxideandmetalassistedandmicrowaveacceleratedevaporativecrystallization AT aslankadir ultrarapidcrystallizationoflalanineusingmonomodemicrowavesindiumtinoxideandmetalassistedandmicrowaveacceleratedevaporativecrystallization |