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Variable localization of Toll-like receptors in human fallopian tube epithelial cells

OBJECTIVE: To determine the localization, expression, and function of Toll-like receptors (TLRs) in fallopian tube epithelial cells. METHODS: The localization of TLRs in fallopian tube epithelial cells was investigated by immunostaining. Surprisingly, the intensity of staining was not equal in the s...

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Autores principales: Amjadi, Fatemehsadat, Zandieh, Zahra, Salehi, Ensieh, Jafari, Reza, Ghasemi, Nasrin, Aflatoonian, Abbas, Fazeli, Alireza, Aflatoonian, Reza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Reproductive Medicine 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5897241/
https://www.ncbi.nlm.nih.gov/pubmed/29662819
http://dx.doi.org/10.5653/cerm.2018.45.1.1
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author Amjadi, Fatemehsadat
Zandieh, Zahra
Salehi, Ensieh
Jafari, Reza
Ghasemi, Nasrin
Aflatoonian, Abbas
Fazeli, Alireza
Aflatoonian, Reza
author_facet Amjadi, Fatemehsadat
Zandieh, Zahra
Salehi, Ensieh
Jafari, Reza
Ghasemi, Nasrin
Aflatoonian, Abbas
Fazeli, Alireza
Aflatoonian, Reza
author_sort Amjadi, Fatemehsadat
collection PubMed
description OBJECTIVE: To determine the localization, expression, and function of Toll-like receptors (TLRs) in fallopian tube epithelial cells. METHODS: The localization of TLRs in fallopian tube epithelial cells was investigated by immunostaining. Surprisingly, the intensity of staining was not equal in the secretory and ciliated cells. After primary cell culture of fallopian tube epithelial cells, ring cloning was used to isolate colonies of ciliated epithelial cells, distinct from non-ciliated epithelial cells. The expression of TLRs 1–10 was examined by quantitative real-time polymerase chain reaction, and protein localization was confirmed by immunostaining. The function of the TLRs was determined by interleukin (IL)-6 and IL-8 production in response to TLR2, TLR3, TLR5, TLR7, and TLR9 ligands. RESULTS: Fallopian tube epithelial cells expressed TLRs 1–10 in a cell-type-specific manner. Exposing fallopian tube epithelial cells to TLR2, TLR3, TLR5, TLR7, and TLR9 agonists induced the secretion of proinflammatory cytokines such as IL-6 and IL-8. CONCLUSION: Our findings suggest that TLR expression in the fallopian tubes is cell-type-specific. According to our results, ciliated cells may play more effective role than non-ciliated cells in the innate immune defense of the fallopian tubes, and in interactions with gametes and embryos.
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spelling pubmed-58972412018-04-16 Variable localization of Toll-like receptors in human fallopian tube epithelial cells Amjadi, Fatemehsadat Zandieh, Zahra Salehi, Ensieh Jafari, Reza Ghasemi, Nasrin Aflatoonian, Abbas Fazeli, Alireza Aflatoonian, Reza Clin Exp Reprod Med Original Article OBJECTIVE: To determine the localization, expression, and function of Toll-like receptors (TLRs) in fallopian tube epithelial cells. METHODS: The localization of TLRs in fallopian tube epithelial cells was investigated by immunostaining. Surprisingly, the intensity of staining was not equal in the secretory and ciliated cells. After primary cell culture of fallopian tube epithelial cells, ring cloning was used to isolate colonies of ciliated epithelial cells, distinct from non-ciliated epithelial cells. The expression of TLRs 1–10 was examined by quantitative real-time polymerase chain reaction, and protein localization was confirmed by immunostaining. The function of the TLRs was determined by interleukin (IL)-6 and IL-8 production in response to TLR2, TLR3, TLR5, TLR7, and TLR9 ligands. RESULTS: Fallopian tube epithelial cells expressed TLRs 1–10 in a cell-type-specific manner. Exposing fallopian tube epithelial cells to TLR2, TLR3, TLR5, TLR7, and TLR9 agonists induced the secretion of proinflammatory cytokines such as IL-6 and IL-8. CONCLUSION: Our findings suggest that TLR expression in the fallopian tubes is cell-type-specific. According to our results, ciliated cells may play more effective role than non-ciliated cells in the innate immune defense of the fallopian tubes, and in interactions with gametes and embryos. The Korean Society for Reproductive Medicine 2018-03 2018-03-30 /pmc/articles/PMC5897241/ /pubmed/29662819 http://dx.doi.org/10.5653/cerm.2018.45.1.1 Text en Copyright © 2018. The Korean Society for Reproductive Medicine http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Amjadi, Fatemehsadat
Zandieh, Zahra
Salehi, Ensieh
Jafari, Reza
Ghasemi, Nasrin
Aflatoonian, Abbas
Fazeli, Alireza
Aflatoonian, Reza
Variable localization of Toll-like receptors in human fallopian tube epithelial cells
title Variable localization of Toll-like receptors in human fallopian tube epithelial cells
title_full Variable localization of Toll-like receptors in human fallopian tube epithelial cells
title_fullStr Variable localization of Toll-like receptors in human fallopian tube epithelial cells
title_full_unstemmed Variable localization of Toll-like receptors in human fallopian tube epithelial cells
title_short Variable localization of Toll-like receptors in human fallopian tube epithelial cells
title_sort variable localization of toll-like receptors in human fallopian tube epithelial cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5897241/
https://www.ncbi.nlm.nih.gov/pubmed/29662819
http://dx.doi.org/10.5653/cerm.2018.45.1.1
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