Comparative analysis of naive, primed and ground state pluripotency in mouse embryonic stem cells originating from the same genetic background

Mouse embryonic stem cells (mESCs) exist in a naive, primed and ground state of pluripotency. While comparative analyses of these pluripotency states have been reported, the mESCs utilized originated from various genetic backgrounds and were derived in different laboratories. mESC derivation in conv...

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Autores principales: Ghimire, Sabitri, Van der Jeught, Margot, Neupane, Jitesh, Roost, Matthias S., Anckaert, Jasper, Popovic, Mina, Van Nieuwerburgh, Filip, Mestdagh, Pieter, Vandesompele, Jo, Deforce, Dieter, Menten, Björn, Chuva de Sousa Lopes, Susana, De Sutter, Petra, Heindryckx, Björn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5897387/
https://www.ncbi.nlm.nih.gov/pubmed/29650979
http://dx.doi.org/10.1038/s41598-018-24051-5
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author Ghimire, Sabitri
Van der Jeught, Margot
Neupane, Jitesh
Roost, Matthias S.
Anckaert, Jasper
Popovic, Mina
Van Nieuwerburgh, Filip
Mestdagh, Pieter
Vandesompele, Jo
Deforce, Dieter
Menten, Björn
Chuva de Sousa Lopes, Susana
De Sutter, Petra
Heindryckx, Björn
author_facet Ghimire, Sabitri
Van der Jeught, Margot
Neupane, Jitesh
Roost, Matthias S.
Anckaert, Jasper
Popovic, Mina
Van Nieuwerburgh, Filip
Mestdagh, Pieter
Vandesompele, Jo
Deforce, Dieter
Menten, Björn
Chuva de Sousa Lopes, Susana
De Sutter, Petra
Heindryckx, Björn
author_sort Ghimire, Sabitri
collection PubMed
description Mouse embryonic stem cells (mESCs) exist in a naive, primed and ground state of pluripotency. While comparative analyses of these pluripotency states have been reported, the mESCs utilized originated from various genetic backgrounds and were derived in different laboratories. mESC derivation in conventional LIF + serum culture conditions is strain dependent, with different genetic backgrounds potentially affecting subsequent stem cell characteristics. In the present study, we performed a comprehensive characterization of naive, primed and ground state mESCs originating from the same genetic background within our laboratory, by comparing their transcriptional profiles. We showed unique transcriptional profiles for naive, primed and ground state mESCs. While naive and ground state mESCs have more similar but not identical profiles, primed state mESCs show a very distinct profile. We further demonstrate that the differentiation propensity of mESCs to specific germ layers is highly dependent on their respective state of pluripotency.
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spelling pubmed-58973872018-04-20 Comparative analysis of naive, primed and ground state pluripotency in mouse embryonic stem cells originating from the same genetic background Ghimire, Sabitri Van der Jeught, Margot Neupane, Jitesh Roost, Matthias S. Anckaert, Jasper Popovic, Mina Van Nieuwerburgh, Filip Mestdagh, Pieter Vandesompele, Jo Deforce, Dieter Menten, Björn Chuva de Sousa Lopes, Susana De Sutter, Petra Heindryckx, Björn Sci Rep Article Mouse embryonic stem cells (mESCs) exist in a naive, primed and ground state of pluripotency. While comparative analyses of these pluripotency states have been reported, the mESCs utilized originated from various genetic backgrounds and were derived in different laboratories. mESC derivation in conventional LIF + serum culture conditions is strain dependent, with different genetic backgrounds potentially affecting subsequent stem cell characteristics. In the present study, we performed a comprehensive characterization of naive, primed and ground state mESCs originating from the same genetic background within our laboratory, by comparing their transcriptional profiles. We showed unique transcriptional profiles for naive, primed and ground state mESCs. While naive and ground state mESCs have more similar but not identical profiles, primed state mESCs show a very distinct profile. We further demonstrate that the differentiation propensity of mESCs to specific germ layers is highly dependent on their respective state of pluripotency. Nature Publishing Group UK 2018-04-12 /pmc/articles/PMC5897387/ /pubmed/29650979 http://dx.doi.org/10.1038/s41598-018-24051-5 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ghimire, Sabitri
Van der Jeught, Margot
Neupane, Jitesh
Roost, Matthias S.
Anckaert, Jasper
Popovic, Mina
Van Nieuwerburgh, Filip
Mestdagh, Pieter
Vandesompele, Jo
Deforce, Dieter
Menten, Björn
Chuva de Sousa Lopes, Susana
De Sutter, Petra
Heindryckx, Björn
Comparative analysis of naive, primed and ground state pluripotency in mouse embryonic stem cells originating from the same genetic background
title Comparative analysis of naive, primed and ground state pluripotency in mouse embryonic stem cells originating from the same genetic background
title_full Comparative analysis of naive, primed and ground state pluripotency in mouse embryonic stem cells originating from the same genetic background
title_fullStr Comparative analysis of naive, primed and ground state pluripotency in mouse embryonic stem cells originating from the same genetic background
title_full_unstemmed Comparative analysis of naive, primed and ground state pluripotency in mouse embryonic stem cells originating from the same genetic background
title_short Comparative analysis of naive, primed and ground state pluripotency in mouse embryonic stem cells originating from the same genetic background
title_sort comparative analysis of naive, primed and ground state pluripotency in mouse embryonic stem cells originating from the same genetic background
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5897387/
https://www.ncbi.nlm.nih.gov/pubmed/29650979
http://dx.doi.org/10.1038/s41598-018-24051-5
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