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New Approach of QuEChERS and GC-MS Triple-Quadrupole for the Determination of Ethyl Carbamate Content in Brazilian cachaças

Cachaça is a popular spirit produced in Brazil, obtained by distillation of fermented sugar cane. Among the contaminants arising from production, ethyl carbamate is a carcinogenic compound that occurs naturally in fermented foods and beverages; in Brazil, the maximum limit established by current leg...

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Autores principales: Guerreiro, Tatiane Melina, Ozawa, Kumi Shiota, Lima, Estela de Oliveira, Melo, Carlos Fernando Odir Rodrigues, de Oliveira, Diogo Noin, Triano, Simone Pereira do Nascimento, Catharino, Rodrigo Ramos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5897439/
https://www.ncbi.nlm.nih.gov/pubmed/29682506
http://dx.doi.org/10.3389/fnut.2018.00021
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author Guerreiro, Tatiane Melina
Ozawa, Kumi Shiota
Lima, Estela de Oliveira
Melo, Carlos Fernando Odir Rodrigues
de Oliveira, Diogo Noin
Triano, Simone Pereira do Nascimento
Catharino, Rodrigo Ramos
author_facet Guerreiro, Tatiane Melina
Ozawa, Kumi Shiota
Lima, Estela de Oliveira
Melo, Carlos Fernando Odir Rodrigues
de Oliveira, Diogo Noin
Triano, Simone Pereira do Nascimento
Catharino, Rodrigo Ramos
author_sort Guerreiro, Tatiane Melina
collection PubMed
description Cachaça is a popular spirit produced in Brazil, obtained by distillation of fermented sugar cane. Among the contaminants arising from production, ethyl carbamate is a carcinogenic compound that occurs naturally in fermented foods and beverages; in Brazil, the maximum limit established by current legislation is 150 µg L(−1). Quality control is usually performed using gas chromatography; however, robustness and reproducibility of quantitative results may be severely impaired, as the addition of 6–30 g L(−1) of sucrose is a common procedure for taste standardization, directly interfering in the results. This work describes the development of a novel method to improve ethyl carbamate quantification in cachaças using a new approach of QuEChERS extraction based on salting-out phenomenon, to effectively separate ethanol from sugar-containing water. Eighteen different brands of cachaça were analyzed. The proposed methodology was able to eliminate components that contaminate the sample flow path in the gas chromatography system, while improving precision and accuracy by using a triple-quadrupole approach, in comparison with the methodology usually employed: direct analysis of cachaça samples with no sample prep. Results indicate that this approach is more effective due to the removal of sugar content, with no impact in costs per analysis.
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spelling pubmed-58974392018-04-20 New Approach of QuEChERS and GC-MS Triple-Quadrupole for the Determination of Ethyl Carbamate Content in Brazilian cachaças Guerreiro, Tatiane Melina Ozawa, Kumi Shiota Lima, Estela de Oliveira Melo, Carlos Fernando Odir Rodrigues de Oliveira, Diogo Noin Triano, Simone Pereira do Nascimento Catharino, Rodrigo Ramos Front Nutr Nutrition Cachaça is a popular spirit produced in Brazil, obtained by distillation of fermented sugar cane. Among the contaminants arising from production, ethyl carbamate is a carcinogenic compound that occurs naturally in fermented foods and beverages; in Brazil, the maximum limit established by current legislation is 150 µg L(−1). Quality control is usually performed using gas chromatography; however, robustness and reproducibility of quantitative results may be severely impaired, as the addition of 6–30 g L(−1) of sucrose is a common procedure for taste standardization, directly interfering in the results. This work describes the development of a novel method to improve ethyl carbamate quantification in cachaças using a new approach of QuEChERS extraction based on salting-out phenomenon, to effectively separate ethanol from sugar-containing water. Eighteen different brands of cachaça were analyzed. The proposed methodology was able to eliminate components that contaminate the sample flow path in the gas chromatography system, while improving precision and accuracy by using a triple-quadrupole approach, in comparison with the methodology usually employed: direct analysis of cachaça samples with no sample prep. Results indicate that this approach is more effective due to the removal of sugar content, with no impact in costs per analysis. Frontiers Media S.A. 2018-04-06 /pmc/articles/PMC5897439/ /pubmed/29682506 http://dx.doi.org/10.3389/fnut.2018.00021 Text en Copyright © 2018 Guerreiro, Ozawa, Lima, Melo, de Oliveira, Triano and Catharino. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Guerreiro, Tatiane Melina
Ozawa, Kumi Shiota
Lima, Estela de Oliveira
Melo, Carlos Fernando Odir Rodrigues
de Oliveira, Diogo Noin
Triano, Simone Pereira do Nascimento
Catharino, Rodrigo Ramos
New Approach of QuEChERS and GC-MS Triple-Quadrupole for the Determination of Ethyl Carbamate Content in Brazilian cachaças
title New Approach of QuEChERS and GC-MS Triple-Quadrupole for the Determination of Ethyl Carbamate Content in Brazilian cachaças
title_full New Approach of QuEChERS and GC-MS Triple-Quadrupole for the Determination of Ethyl Carbamate Content in Brazilian cachaças
title_fullStr New Approach of QuEChERS and GC-MS Triple-Quadrupole for the Determination of Ethyl Carbamate Content in Brazilian cachaças
title_full_unstemmed New Approach of QuEChERS and GC-MS Triple-Quadrupole for the Determination of Ethyl Carbamate Content in Brazilian cachaças
title_short New Approach of QuEChERS and GC-MS Triple-Quadrupole for the Determination of Ethyl Carbamate Content in Brazilian cachaças
title_sort new approach of quechers and gc-ms triple-quadrupole for the determination of ethyl carbamate content in brazilian cachaças
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5897439/
https://www.ncbi.nlm.nih.gov/pubmed/29682506
http://dx.doi.org/10.3389/fnut.2018.00021
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