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Innovations in biomedical nanoengineering: nanowell array biosensor
Nanostructured biosensors have pioneered biomedical engineering by providing highly sensitive analyses of biomolecules. The nanowell array (NWA)-based biosensing platform is particularly innovative, where the small size of NWs within the array permits extremely profound sensing of a small quantity o...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Singapore
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5897454/ https://www.ncbi.nlm.nih.gov/pubmed/29670832 http://dx.doi.org/10.1186/s40580-018-0141-6 |
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author | Seo, YoungTae Jeong, Sunil Lee, JuKyung Choi, Hak Soo Kim, Jonghan Lee, HeaYeon |
author_facet | Seo, YoungTae Jeong, Sunil Lee, JuKyung Choi, Hak Soo Kim, Jonghan Lee, HeaYeon |
author_sort | Seo, YoungTae |
collection | PubMed |
description | Nanostructured biosensors have pioneered biomedical engineering by providing highly sensitive analyses of biomolecules. The nanowell array (NWA)-based biosensing platform is particularly innovative, where the small size of NWs within the array permits extremely profound sensing of a small quantity of biomolecules. Undoubtedly, the NWA geometry of a gently-sloped vertical wall is critical for selective docking of specific proteins without capillary resistances, and nanoprocessing has contributed to the fabrication of NWA electrodes on gold substrate such as molding process, e-beam lithography, and krypton-fluoride (KrF) stepper semiconductor method. The Lee group at the Mara Nanotech has established this NW-based biosensing technology during the past two decades by engineering highly sensitive electrochemical sensors and providing a broad range of detection methods from large molecules (e.g., cells or proteins) to small molecules (e.g., DNA and RNA). Nanosized gold dots in the NWA enhance the detection of electrochemical biosensing to the range of zeptomoles in precision against the complementary target DNA molecules. In this review, we discuss recent innovations in biomedical nanoengineering with a specific focus on novel NWA-based biosensors. We also describe our continuous efforts in achieving a label-free detection without non-specific binding while maintaining the activity and stability of immobilized biomolecules. This research can lay the foundation of a new platform for biomedical nanoengineering systems. |
format | Online Article Text |
id | pubmed-5897454 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Springer Singapore |
record_format | MEDLINE/PubMed |
spelling | pubmed-58974542018-04-16 Innovations in biomedical nanoengineering: nanowell array biosensor Seo, YoungTae Jeong, Sunil Lee, JuKyung Choi, Hak Soo Kim, Jonghan Lee, HeaYeon Nano Converg Review Nanostructured biosensors have pioneered biomedical engineering by providing highly sensitive analyses of biomolecules. The nanowell array (NWA)-based biosensing platform is particularly innovative, where the small size of NWs within the array permits extremely profound sensing of a small quantity of biomolecules. Undoubtedly, the NWA geometry of a gently-sloped vertical wall is critical for selective docking of specific proteins without capillary resistances, and nanoprocessing has contributed to the fabrication of NWA electrodes on gold substrate such as molding process, e-beam lithography, and krypton-fluoride (KrF) stepper semiconductor method. The Lee group at the Mara Nanotech has established this NW-based biosensing technology during the past two decades by engineering highly sensitive electrochemical sensors and providing a broad range of detection methods from large molecules (e.g., cells or proteins) to small molecules (e.g., DNA and RNA). Nanosized gold dots in the NWA enhance the detection of electrochemical biosensing to the range of zeptomoles in precision against the complementary target DNA molecules. In this review, we discuss recent innovations in biomedical nanoengineering with a specific focus on novel NWA-based biosensors. We also describe our continuous efforts in achieving a label-free detection without non-specific binding while maintaining the activity and stability of immobilized biomolecules. This research can lay the foundation of a new platform for biomedical nanoengineering systems. Springer Singapore 2018-04-11 /pmc/articles/PMC5897454/ /pubmed/29670832 http://dx.doi.org/10.1186/s40580-018-0141-6 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Review Seo, YoungTae Jeong, Sunil Lee, JuKyung Choi, Hak Soo Kim, Jonghan Lee, HeaYeon Innovations in biomedical nanoengineering: nanowell array biosensor |
title | Innovations in biomedical nanoengineering: nanowell array biosensor |
title_full | Innovations in biomedical nanoengineering: nanowell array biosensor |
title_fullStr | Innovations in biomedical nanoengineering: nanowell array biosensor |
title_full_unstemmed | Innovations in biomedical nanoengineering: nanowell array biosensor |
title_short | Innovations in biomedical nanoengineering: nanowell array biosensor |
title_sort | innovations in biomedical nanoengineering: nanowell array biosensor |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5897454/ https://www.ncbi.nlm.nih.gov/pubmed/29670832 http://dx.doi.org/10.1186/s40580-018-0141-6 |
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