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Down-regulation of miR-10a-5p promotes proliferation and restricts apoptosis via targeting T-box transcription factor 5 in inflamed synoviocytes

Synoviocytes from rheumatoid arthritis (RA) patients share certain features with tumor cells, such as over proliferation and invasion. Anomalous microRNA (miRNA) expression may participate in the pathogenesis of RA in different ways. The objective of the present study was to observe the role of miR-...

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Autores principales: Hussain, Nazim, Zhu, Wenhua, Jiang, Congshan, Xu, Jing, Geng, Manman, Wu, Xiaoying, Hussain, Safdar, Wang, Bo, Rajoka, Muhammad Shahid Riaz, Li, Yue, Tian, Juan, Meng, Liesu, Lu, Shemin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5897746/
https://www.ncbi.nlm.nih.gov/pubmed/29545315
http://dx.doi.org/10.1042/BSR20180003
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author Hussain, Nazim
Zhu, Wenhua
Jiang, Congshan
Xu, Jing
Geng, Manman
Wu, Xiaoying
Hussain, Safdar
Wang, Bo
Rajoka, Muhammad Shahid Riaz
Li, Yue
Tian, Juan
Meng, Liesu
Lu, Shemin
author_facet Hussain, Nazim
Zhu, Wenhua
Jiang, Congshan
Xu, Jing
Geng, Manman
Wu, Xiaoying
Hussain, Safdar
Wang, Bo
Rajoka, Muhammad Shahid Riaz
Li, Yue
Tian, Juan
Meng, Liesu
Lu, Shemin
author_sort Hussain, Nazim
collection PubMed
description Synoviocytes from rheumatoid arthritis (RA) patients share certain features with tumor cells, such as over proliferation and invasion. Anomalous microRNA (miRNA) expression may participate in the pathogenesis of RA in different ways. The objective of the present study was to observe the role of miR-10a-5p targeting T-box transcription factor 5 (TBX5) gene on synoviocyte proliferation and apoptosis in RA. Human synovial sarcoma cell line, SW982 cells stimulating with interleukin-1β (IL-1β) were transfected with miR-10a-5p mimic and siRNA of TBX5. The real-time quantitative polymerase chain reaction (RT-qPCR) and Western blotting analysis were used to evaluate the expression level of miR-10a-5p and TBX5 in SW982 cells respectively. Further, the proliferation and apoptosis of SW982 cells after treatment were determined by cell counting kit (CCK-8) and flow cytometry analysis respectively. We found that the miR-10a-5p showed down-regulated while TBX5 showed up-regulated expression in synoviocytes after stimulation with IL-1β. The miR-10a-5p mimic treatment showed a decline in cell proliferation while the increased rate of cell apoptosis as compared with control. Moreover, knockdown of TBX5 favored the apoptosis and reduced the cell proliferation as compared with control group. We conclude that down-regulation of miR-10a-5p promotes proliferation and restricts apoptosis via targeting TBX5 in inflamed synoviocytes.
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spelling pubmed-58977462018-04-17 Down-regulation of miR-10a-5p promotes proliferation and restricts apoptosis via targeting T-box transcription factor 5 in inflamed synoviocytes Hussain, Nazim Zhu, Wenhua Jiang, Congshan Xu, Jing Geng, Manman Wu, Xiaoying Hussain, Safdar Wang, Bo Rajoka, Muhammad Shahid Riaz Li, Yue Tian, Juan Meng, Liesu Lu, Shemin Biosci Rep Research Articles Synoviocytes from rheumatoid arthritis (RA) patients share certain features with tumor cells, such as over proliferation and invasion. Anomalous microRNA (miRNA) expression may participate in the pathogenesis of RA in different ways. The objective of the present study was to observe the role of miR-10a-5p targeting T-box transcription factor 5 (TBX5) gene on synoviocyte proliferation and apoptosis in RA. Human synovial sarcoma cell line, SW982 cells stimulating with interleukin-1β (IL-1β) were transfected with miR-10a-5p mimic and siRNA of TBX5. The real-time quantitative polymerase chain reaction (RT-qPCR) and Western blotting analysis were used to evaluate the expression level of miR-10a-5p and TBX5 in SW982 cells respectively. Further, the proliferation and apoptosis of SW982 cells after treatment were determined by cell counting kit (CCK-8) and flow cytometry analysis respectively. We found that the miR-10a-5p showed down-regulated while TBX5 showed up-regulated expression in synoviocytes after stimulation with IL-1β. The miR-10a-5p mimic treatment showed a decline in cell proliferation while the increased rate of cell apoptosis as compared with control. Moreover, knockdown of TBX5 favored the apoptosis and reduced the cell proliferation as compared with control group. We conclude that down-regulation of miR-10a-5p promotes proliferation and restricts apoptosis via targeting TBX5 in inflamed synoviocytes. Portland Press Ltd. 2018-04-13 /pmc/articles/PMC5897746/ /pubmed/29545315 http://dx.doi.org/10.1042/BSR20180003 Text en © 2018 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Hussain, Nazim
Zhu, Wenhua
Jiang, Congshan
Xu, Jing
Geng, Manman
Wu, Xiaoying
Hussain, Safdar
Wang, Bo
Rajoka, Muhammad Shahid Riaz
Li, Yue
Tian, Juan
Meng, Liesu
Lu, Shemin
Down-regulation of miR-10a-5p promotes proliferation and restricts apoptosis via targeting T-box transcription factor 5 in inflamed synoviocytes
title Down-regulation of miR-10a-5p promotes proliferation and restricts apoptosis via targeting T-box transcription factor 5 in inflamed synoviocytes
title_full Down-regulation of miR-10a-5p promotes proliferation and restricts apoptosis via targeting T-box transcription factor 5 in inflamed synoviocytes
title_fullStr Down-regulation of miR-10a-5p promotes proliferation and restricts apoptosis via targeting T-box transcription factor 5 in inflamed synoviocytes
title_full_unstemmed Down-regulation of miR-10a-5p promotes proliferation and restricts apoptosis via targeting T-box transcription factor 5 in inflamed synoviocytes
title_short Down-regulation of miR-10a-5p promotes proliferation and restricts apoptosis via targeting T-box transcription factor 5 in inflamed synoviocytes
title_sort down-regulation of mir-10a-5p promotes proliferation and restricts apoptosis via targeting t-box transcription factor 5 in inflamed synoviocytes
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5897746/
https://www.ncbi.nlm.nih.gov/pubmed/29545315
http://dx.doi.org/10.1042/BSR20180003
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