A MILI-independent piRNA biogenesis pathway empowers partial germline reprogramming

In mice, the PIWI-piRNA pathway is essential to re-establish transposon silencing during male germline reprogramming. The cytoplasmic PIWI protein MILI mediates piRNA-guided transposon RNA cleavage as well as piRNA amplification. MIWI2-bound piRNAs and its nuclear localization are proposed to be dep...

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Detalles Bibliográficos
Autores principales: Vasilauskaitė, Lina, Vitsios, Dimitrios, Berrens, Rebecca, Carrieri, Claudia, Reik, Wolf, Enright, Anton J, O’Carroll, Dónal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5898609/
https://www.ncbi.nlm.nih.gov/pubmed/28530707
http://dx.doi.org/10.1038/nsmb.3413
Descripción
Sumario:In mice, the PIWI-piRNA pathway is essential to re-establish transposon silencing during male germline reprogramming. The cytoplasmic PIWI protein MILI mediates piRNA-guided transposon RNA cleavage as well as piRNA amplification. MIWI2-bound piRNAs and its nuclear localization are proposed to be dependent upon MILI function. Here, we demonstrate the existence of a piRNA biogenesis pathway that in the absence of MILI sustains partial MIWI2 function and reprogramming activity.

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