A MILI-independent piRNA biogenesis pathway empowers partial germline reprogramming

In mice, the PIWI-piRNA pathway is essential to re-establish transposon silencing during male germline reprogramming. The cytoplasmic PIWI protein MILI mediates piRNA-guided transposon RNA cleavage as well as piRNA amplification. MIWI2-bound piRNAs and its nuclear localization are proposed to be dep...

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Autores principales: Vasilauskaitė, Lina, Vitsios, Dimitrios, Berrens, Rebecca, Carrieri, Claudia, Reik, Wolf, Enright, Anton J, O’Carroll, Dónal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5898609/
https://www.ncbi.nlm.nih.gov/pubmed/28530707
http://dx.doi.org/10.1038/nsmb.3413
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author Vasilauskaitė, Lina
Vitsios, Dimitrios
Berrens, Rebecca
Carrieri, Claudia
Reik, Wolf
Enright, Anton J
O’Carroll, Dónal
author_facet Vasilauskaitė, Lina
Vitsios, Dimitrios
Berrens, Rebecca
Carrieri, Claudia
Reik, Wolf
Enright, Anton J
O’Carroll, Dónal
author_sort Vasilauskaitė, Lina
collection PubMed
description In mice, the PIWI-piRNA pathway is essential to re-establish transposon silencing during male germline reprogramming. The cytoplasmic PIWI protein MILI mediates piRNA-guided transposon RNA cleavage as well as piRNA amplification. MIWI2-bound piRNAs and its nuclear localization are proposed to be dependent upon MILI function. Here, we demonstrate the existence of a piRNA biogenesis pathway that in the absence of MILI sustains partial MIWI2 function and reprogramming activity.
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spelling pubmed-58986092018-04-13 A MILI-independent piRNA biogenesis pathway empowers partial germline reprogramming Vasilauskaitė, Lina Vitsios, Dimitrios Berrens, Rebecca Carrieri, Claudia Reik, Wolf Enright, Anton J O’Carroll, Dónal Nat Struct Mol Biol Article In mice, the PIWI-piRNA pathway is essential to re-establish transposon silencing during male germline reprogramming. The cytoplasmic PIWI protein MILI mediates piRNA-guided transposon RNA cleavage as well as piRNA amplification. MIWI2-bound piRNAs and its nuclear localization are proposed to be dependent upon MILI function. Here, we demonstrate the existence of a piRNA biogenesis pathway that in the absence of MILI sustains partial MIWI2 function and reprogramming activity. 2017-05-22 2017-07 /pmc/articles/PMC5898609/ /pubmed/28530707 http://dx.doi.org/10.1038/nsmb.3413 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Vasilauskaitė, Lina
Vitsios, Dimitrios
Berrens, Rebecca
Carrieri, Claudia
Reik, Wolf
Enright, Anton J
O’Carroll, Dónal
A MILI-independent piRNA biogenesis pathway empowers partial germline reprogramming
title A MILI-independent piRNA biogenesis pathway empowers partial germline reprogramming
title_full A MILI-independent piRNA biogenesis pathway empowers partial germline reprogramming
title_fullStr A MILI-independent piRNA biogenesis pathway empowers partial germline reprogramming
title_full_unstemmed A MILI-independent piRNA biogenesis pathway empowers partial germline reprogramming
title_short A MILI-independent piRNA biogenesis pathway empowers partial germline reprogramming
title_sort mili-independent pirna biogenesis pathway empowers partial germline reprogramming
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5898609/
https://www.ncbi.nlm.nih.gov/pubmed/28530707
http://dx.doi.org/10.1038/nsmb.3413
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