Biobeam—Multiplexed wave-optical simulations of light-sheet microscopy

Sample-induced image-degradation remains an intricate wave-optical problem in light-sheet microscopy. Here we present biobeam, an open-source software package that enables simulation of operational light-sheet microscopes by combining data from 10(5)–10(6) multiplexed and GPU-accelerated point-sprea...

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Autores principales: Weigert, Martin, Subramanian, Kaushikaram, Bundschuh, Sebastian T., Myers, Eugene W., Kreysing, Moritz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5898703/
https://www.ncbi.nlm.nih.gov/pubmed/29652879
http://dx.doi.org/10.1371/journal.pcbi.1006079
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author Weigert, Martin
Subramanian, Kaushikaram
Bundschuh, Sebastian T.
Myers, Eugene W.
Kreysing, Moritz
author_facet Weigert, Martin
Subramanian, Kaushikaram
Bundschuh, Sebastian T.
Myers, Eugene W.
Kreysing, Moritz
author_sort Weigert, Martin
collection PubMed
description Sample-induced image-degradation remains an intricate wave-optical problem in light-sheet microscopy. Here we present biobeam, an open-source software package that enables simulation of operational light-sheet microscopes by combining data from 10(5)–10(6) multiplexed and GPU-accelerated point-spread-function calculations. The wave-optical nature of these simulations leads to the faithful reproduction of spatially varying aberrations, diffraction artifacts, geometric image distortions, adaptive optics, and emergent wave-optical phenomena, and renders image-formation in light-sheet microscopy computationally tractable.
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spelling pubmed-58987032018-05-06 Biobeam—Multiplexed wave-optical simulations of light-sheet microscopy Weigert, Martin Subramanian, Kaushikaram Bundschuh, Sebastian T. Myers, Eugene W. Kreysing, Moritz PLoS Comput Biol Research Article Sample-induced image-degradation remains an intricate wave-optical problem in light-sheet microscopy. Here we present biobeam, an open-source software package that enables simulation of operational light-sheet microscopes by combining data from 10(5)–10(6) multiplexed and GPU-accelerated point-spread-function calculations. The wave-optical nature of these simulations leads to the faithful reproduction of spatially varying aberrations, diffraction artifacts, geometric image distortions, adaptive optics, and emergent wave-optical phenomena, and renders image-formation in light-sheet microscopy computationally tractable. Public Library of Science 2018-04-13 /pmc/articles/PMC5898703/ /pubmed/29652879 http://dx.doi.org/10.1371/journal.pcbi.1006079 Text en © 2018 Weigert et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Weigert, Martin
Subramanian, Kaushikaram
Bundschuh, Sebastian T.
Myers, Eugene W.
Kreysing, Moritz
Biobeam—Multiplexed wave-optical simulations of light-sheet microscopy
title Biobeam—Multiplexed wave-optical simulations of light-sheet microscopy
title_full Biobeam—Multiplexed wave-optical simulations of light-sheet microscopy
title_fullStr Biobeam—Multiplexed wave-optical simulations of light-sheet microscopy
title_full_unstemmed Biobeam—Multiplexed wave-optical simulations of light-sheet microscopy
title_short Biobeam—Multiplexed wave-optical simulations of light-sheet microscopy
title_sort biobeam—multiplexed wave-optical simulations of light-sheet microscopy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5898703/
https://www.ncbi.nlm.nih.gov/pubmed/29652879
http://dx.doi.org/10.1371/journal.pcbi.1006079
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