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Integrated dual-tomography for refractive index analysis of free-floating single living cell with isotropic superresolution
Digital holographic microtomography is a promising technique for three-dimensional (3D) measurement of the refractive index (RI) profiles of biological specimens. Measurement of the RI distribution of a free-floating single living cell with an isotropic superresolution had not previously been accomp...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5899089/ https://www.ncbi.nlm.nih.gov/pubmed/29654324 http://dx.doi.org/10.1038/s41598-018-24408-w |
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author | B., Vinoth Lai, Xin-Ji Lin, Yu-Chih Tu, Han-Yen Cheng, Chau-Jern |
author_facet | B., Vinoth Lai, Xin-Ji Lin, Yu-Chih Tu, Han-Yen Cheng, Chau-Jern |
author_sort | B., Vinoth |
collection | PubMed |
description | Digital holographic microtomography is a promising technique for three-dimensional (3D) measurement of the refractive index (RI) profiles of biological specimens. Measurement of the RI distribution of a free-floating single living cell with an isotropic superresolution had not previously been accomplished. To the best of our knowledge, this is the first study focusing on the development of an integrated dual-tomographic (IDT) imaging system for RI measurement of an unlabelled free-floating single living cell with an isotropic superresolution by combining the spatial frequencies of full-angle specimen rotation with those of beam rotation. A novel ‘UFO’ (unidentified flying object) like shaped coherent transfer function is obtained. The IDT imaging system does not require any complex image-processing algorithm for 3D reconstruction. The working principle was successfully demonstrated and a 3D RI profile of a single living cell, Candida rugosa, was obtained with an isotropic superresolution. This technology is expected to set a benchmark for free-floating single live sample measurements without labeling or any special sample preparations for the experiments. |
format | Online Article Text |
id | pubmed-5899089 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-58990892018-04-20 Integrated dual-tomography for refractive index analysis of free-floating single living cell with isotropic superresolution B., Vinoth Lai, Xin-Ji Lin, Yu-Chih Tu, Han-Yen Cheng, Chau-Jern Sci Rep Article Digital holographic microtomography is a promising technique for three-dimensional (3D) measurement of the refractive index (RI) profiles of biological specimens. Measurement of the RI distribution of a free-floating single living cell with an isotropic superresolution had not previously been accomplished. To the best of our knowledge, this is the first study focusing on the development of an integrated dual-tomographic (IDT) imaging system for RI measurement of an unlabelled free-floating single living cell with an isotropic superresolution by combining the spatial frequencies of full-angle specimen rotation with those of beam rotation. A novel ‘UFO’ (unidentified flying object) like shaped coherent transfer function is obtained. The IDT imaging system does not require any complex image-processing algorithm for 3D reconstruction. The working principle was successfully demonstrated and a 3D RI profile of a single living cell, Candida rugosa, was obtained with an isotropic superresolution. This technology is expected to set a benchmark for free-floating single live sample measurements without labeling or any special sample preparations for the experiments. Nature Publishing Group UK 2018-04-13 /pmc/articles/PMC5899089/ /pubmed/29654324 http://dx.doi.org/10.1038/s41598-018-24408-w Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article B., Vinoth Lai, Xin-Ji Lin, Yu-Chih Tu, Han-Yen Cheng, Chau-Jern Integrated dual-tomography for refractive index analysis of free-floating single living cell with isotropic superresolution |
title | Integrated dual-tomography for refractive index analysis of free-floating single living cell with isotropic superresolution |
title_full | Integrated dual-tomography for refractive index analysis of free-floating single living cell with isotropic superresolution |
title_fullStr | Integrated dual-tomography for refractive index analysis of free-floating single living cell with isotropic superresolution |
title_full_unstemmed | Integrated dual-tomography for refractive index analysis of free-floating single living cell with isotropic superresolution |
title_short | Integrated dual-tomography for refractive index analysis of free-floating single living cell with isotropic superresolution |
title_sort | integrated dual-tomography for refractive index analysis of free-floating single living cell with isotropic superresolution |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5899089/ https://www.ncbi.nlm.nih.gov/pubmed/29654324 http://dx.doi.org/10.1038/s41598-018-24408-w |
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