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Evaluation of a therapy for Idiopathic Chronic Enterocolitis in rhesus macaques (Macaca mulatta) and linked microbial community correlates
Idiopathic chronic enterocolitis (ICE) is one of the most commonly encountered and difficult to manage diseases of captive rhesus macaques (Macaca mulatta). The etiology is not well understood, but perturbations in gut microbial communities have been implicated. Here we evaluated the effects of a 14...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5899420/ https://www.ncbi.nlm.nih.gov/pubmed/29666764 http://dx.doi.org/10.7717/peerj.4612 |
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author | Taylor, Joshua M. Clarke, Erik L. Baker, Kate Lauder, Abigail Kim, Dorothy Bailey, Aubrey Wu, Gary D. Collman, Ronald G. Doyle-Meyers, Lara Russell-Lodrigue, Kasi Blanchard, James Bushman, Frederic D. Bohm, Rudolf |
author_facet | Taylor, Joshua M. Clarke, Erik L. Baker, Kate Lauder, Abigail Kim, Dorothy Bailey, Aubrey Wu, Gary D. Collman, Ronald G. Doyle-Meyers, Lara Russell-Lodrigue, Kasi Blanchard, James Bushman, Frederic D. Bohm, Rudolf |
author_sort | Taylor, Joshua M. |
collection | PubMed |
description | Idiopathic chronic enterocolitis (ICE) is one of the most commonly encountered and difficult to manage diseases of captive rhesus macaques (Macaca mulatta). The etiology is not well understood, but perturbations in gut microbial communities have been implicated. Here we evaluated the effects of a 14-day course of vancomycin, neomycin, and fluconazole on animals affected with ICE, comparing treated, untreated, and healthy animals. We performed microbiome analysis on duodenal and colonic mucosal samples and feces in order to probe bacterial and/or fungal taxa potentially associated with ICE. All treated animals showed a significant and long-lasting improvement in stool consistency over time when compared to untreated and healthy controls. Microbiome analysis revealed trends associating bacterial community composition with ICE, particularly lineages of the Lactobacillaceae family. Sequencing of DNA from macaque food biscuits revealed that fungal sequences recovered from stool were dominated by yeast-derived food additives; in contrast, bacteria in stool appeared to be authentic gut residents. In conclusion, while validation in larger cohorts is needed, the treatment described here was associated with significantly improved clinical signs; results suggested possible correlates of microbiome structure with disease, though no strong associations were detected between single microbes and ICE. |
format | Online Article Text |
id | pubmed-5899420 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-58994202018-04-17 Evaluation of a therapy for Idiopathic Chronic Enterocolitis in rhesus macaques (Macaca mulatta) and linked microbial community correlates Taylor, Joshua M. Clarke, Erik L. Baker, Kate Lauder, Abigail Kim, Dorothy Bailey, Aubrey Wu, Gary D. Collman, Ronald G. Doyle-Meyers, Lara Russell-Lodrigue, Kasi Blanchard, James Bushman, Frederic D. Bohm, Rudolf PeerJ Microbiology Idiopathic chronic enterocolitis (ICE) is one of the most commonly encountered and difficult to manage diseases of captive rhesus macaques (Macaca mulatta). The etiology is not well understood, but perturbations in gut microbial communities have been implicated. Here we evaluated the effects of a 14-day course of vancomycin, neomycin, and fluconazole on animals affected with ICE, comparing treated, untreated, and healthy animals. We performed microbiome analysis on duodenal and colonic mucosal samples and feces in order to probe bacterial and/or fungal taxa potentially associated with ICE. All treated animals showed a significant and long-lasting improvement in stool consistency over time when compared to untreated and healthy controls. Microbiome analysis revealed trends associating bacterial community composition with ICE, particularly lineages of the Lactobacillaceae family. Sequencing of DNA from macaque food biscuits revealed that fungal sequences recovered from stool were dominated by yeast-derived food additives; in contrast, bacteria in stool appeared to be authentic gut residents. In conclusion, while validation in larger cohorts is needed, the treatment described here was associated with significantly improved clinical signs; results suggested possible correlates of microbiome structure with disease, though no strong associations were detected between single microbes and ICE. PeerJ Inc. 2018-04-11 /pmc/articles/PMC5899420/ /pubmed/29666764 http://dx.doi.org/10.7717/peerj.4612 Text en ©2018 Taylor et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Microbiology Taylor, Joshua M. Clarke, Erik L. Baker, Kate Lauder, Abigail Kim, Dorothy Bailey, Aubrey Wu, Gary D. Collman, Ronald G. Doyle-Meyers, Lara Russell-Lodrigue, Kasi Blanchard, James Bushman, Frederic D. Bohm, Rudolf Evaluation of a therapy for Idiopathic Chronic Enterocolitis in rhesus macaques (Macaca mulatta) and linked microbial community correlates |
title | Evaluation of a therapy for Idiopathic Chronic Enterocolitis in rhesus macaques (Macaca mulatta) and linked microbial community correlates |
title_full | Evaluation of a therapy for Idiopathic Chronic Enterocolitis in rhesus macaques (Macaca mulatta) and linked microbial community correlates |
title_fullStr | Evaluation of a therapy for Idiopathic Chronic Enterocolitis in rhesus macaques (Macaca mulatta) and linked microbial community correlates |
title_full_unstemmed | Evaluation of a therapy for Idiopathic Chronic Enterocolitis in rhesus macaques (Macaca mulatta) and linked microbial community correlates |
title_short | Evaluation of a therapy for Idiopathic Chronic Enterocolitis in rhesus macaques (Macaca mulatta) and linked microbial community correlates |
title_sort | evaluation of a therapy for idiopathic chronic enterocolitis in rhesus macaques (macaca mulatta) and linked microbial community correlates |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5899420/ https://www.ncbi.nlm.nih.gov/pubmed/29666764 http://dx.doi.org/10.7717/peerj.4612 |
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