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Clock-dependent chromatin topology modulates circadian transcription and behavior
The circadian clock in animals orchestrates widespread oscillatory gene expression programs, which underlie 24-h rhythms in behavior and physiology. Several studies have shown the possible roles of transcription factors and chromatin marks in controlling cyclic gene expression. However, how daily ac...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5900709/ https://www.ncbi.nlm.nih.gov/pubmed/29572261 http://dx.doi.org/10.1101/gad.312397.118 |
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author | Mermet, Jérôme Yeung, Jake Hurni, Clémence Mauvoisin, Daniel Gustafson, Kyle Jouffe, Céline Nicolas, Damien Emmenegger, Yann Gobet, Cédric Franken, Paul Gachon, Frédéric Naef, Félix |
author_facet | Mermet, Jérôme Yeung, Jake Hurni, Clémence Mauvoisin, Daniel Gustafson, Kyle Jouffe, Céline Nicolas, Damien Emmenegger, Yann Gobet, Cédric Franken, Paul Gachon, Frédéric Naef, Félix |
author_sort | Mermet, Jérôme |
collection | PubMed |
description | The circadian clock in animals orchestrates widespread oscillatory gene expression programs, which underlie 24-h rhythms in behavior and physiology. Several studies have shown the possible roles of transcription factors and chromatin marks in controlling cyclic gene expression. However, how daily active enhancers modulate rhythmic gene transcription in mammalian tissues is not known. Using circular chromosome conformation capture (4C) combined with sequencing (4C-seq), we discovered oscillatory promoter–enhancer interactions along the 24-h cycle in the mouse liver and kidney. Rhythms in chromatin interactions were abolished in arrhythmic Bmal1 knockout mice. Deleting a contacted intronic enhancer element in the Cryptochrome 1 (Cry1) gene was sufficient to compromise the rhythmic chromatin contacts in tissues. Moreover, the deletion reduced the daily dynamics of Cry1 transcriptional burst frequency and, remarkably, shortened the circadian period of locomotor activity rhythms. Our results establish oscillating and clock-controlled promoter–enhancer looping as a regulatory layer underlying circadian transcription and behavior. |
format | Online Article Text |
id | pubmed-5900709 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-59007092018-05-04 Clock-dependent chromatin topology modulates circadian transcription and behavior Mermet, Jérôme Yeung, Jake Hurni, Clémence Mauvoisin, Daniel Gustafson, Kyle Jouffe, Céline Nicolas, Damien Emmenegger, Yann Gobet, Cédric Franken, Paul Gachon, Frédéric Naef, Félix Genes Dev Research Paper The circadian clock in animals orchestrates widespread oscillatory gene expression programs, which underlie 24-h rhythms in behavior and physiology. Several studies have shown the possible roles of transcription factors and chromatin marks in controlling cyclic gene expression. However, how daily active enhancers modulate rhythmic gene transcription in mammalian tissues is not known. Using circular chromosome conformation capture (4C) combined with sequencing (4C-seq), we discovered oscillatory promoter–enhancer interactions along the 24-h cycle in the mouse liver and kidney. Rhythms in chromatin interactions were abolished in arrhythmic Bmal1 knockout mice. Deleting a contacted intronic enhancer element in the Cryptochrome 1 (Cry1) gene was sufficient to compromise the rhythmic chromatin contacts in tissues. Moreover, the deletion reduced the daily dynamics of Cry1 transcriptional burst frequency and, remarkably, shortened the circadian period of locomotor activity rhythms. Our results establish oscillating and clock-controlled promoter–enhancer looping as a regulatory layer underlying circadian transcription and behavior. Cold Spring Harbor Laboratory Press 2018-03-01 /pmc/articles/PMC5900709/ /pubmed/29572261 http://dx.doi.org/10.1101/gad.312397.118 Text en © 2018 Mermet et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by/4.0/ This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Paper Mermet, Jérôme Yeung, Jake Hurni, Clémence Mauvoisin, Daniel Gustafson, Kyle Jouffe, Céline Nicolas, Damien Emmenegger, Yann Gobet, Cédric Franken, Paul Gachon, Frédéric Naef, Félix Clock-dependent chromatin topology modulates circadian transcription and behavior |
title | Clock-dependent chromatin topology modulates circadian transcription and behavior |
title_full | Clock-dependent chromatin topology modulates circadian transcription and behavior |
title_fullStr | Clock-dependent chromatin topology modulates circadian transcription and behavior |
title_full_unstemmed | Clock-dependent chromatin topology modulates circadian transcription and behavior |
title_short | Clock-dependent chromatin topology modulates circadian transcription and behavior |
title_sort | clock-dependent chromatin topology modulates circadian transcription and behavior |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5900709/ https://www.ncbi.nlm.nih.gov/pubmed/29572261 http://dx.doi.org/10.1101/gad.312397.118 |
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