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Immunolocalization of muscarinic M1 receptor in the rat medial prefrontal cortex

The medial prefrontal cortex (mPFC) has been considered to participate in many higher cognitive functions, such as memory formation and spatial navigation. These cognitive functions are modulated by cholinergic afferents via muscarinic acetylcholine receptors. Previous pharmacological studies have s...

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Autores principales: Oda, Satoko, Tsuneoka, Yousuke, Yoshida, Sachine, Adachi‐Akahane, Satomi, Ito, Masanori, Kuroda, Masaru, Funato, Hiromasa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5900831/
https://www.ncbi.nlm.nih.gov/pubmed/29424434
http://dx.doi.org/10.1002/cne.24409
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author Oda, Satoko
Tsuneoka, Yousuke
Yoshida, Sachine
Adachi‐Akahane, Satomi
Ito, Masanori
Kuroda, Masaru
Funato, Hiromasa
author_facet Oda, Satoko
Tsuneoka, Yousuke
Yoshida, Sachine
Adachi‐Akahane, Satomi
Ito, Masanori
Kuroda, Masaru
Funato, Hiromasa
author_sort Oda, Satoko
collection PubMed
description The medial prefrontal cortex (mPFC) has been considered to participate in many higher cognitive functions, such as memory formation and spatial navigation. These cognitive functions are modulated by cholinergic afferents via muscarinic acetylcholine receptors. Previous pharmacological studies have strongly suggested that the M1 receptor (M1R) is the most important subtype among muscarinic receptors to perform these cognitive functions. Actually, M1R is abundant in mPFC. However, the proportion of somata containing M1R among cortical cellular types, and the precise intracellular localization of M1R remain unclear. In this study, to clarify the precise immunolocalization of M1R in rat mPFC, we examined three major cellular types, pyramidal neurons, inhibitory neurons, and astrocytes. M1R immunopositivity signals were found in the majority of the somata of both pyramidal neurons and inhibitory neurons. In pyramidal neurons, strong M1R immunopositivity signals were usually found throughout their somata and dendrites including spines. On the other hand, the signal strength of M1R immunopositivity in the somata of inhibitory neurons significantly varied. Some neurons showed strong signals. Whereas about 40% of GAD67‐immunopositive neurons and 30% of parvalbumin‐immunopositive neurons (PV neurons) showed only weak signals. In PV neurons, M1R immunopositivity signals were preferentially distributed in somata. Furthermore, we found that many astrocytes showed substantial M1R immunopositivity signals. These signals were also mainly distributed in their somata. Thus, the distribution pattern of M1R markedly differs between cellular types. This difference might underlie the cholinergic modulation of higher cognitive functions subserved by mPFC.
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spelling pubmed-59008312018-04-23 Immunolocalization of muscarinic M1 receptor in the rat medial prefrontal cortex Oda, Satoko Tsuneoka, Yousuke Yoshida, Sachine Adachi‐Akahane, Satomi Ito, Masanori Kuroda, Masaru Funato, Hiromasa J Comp Neurol Research Articles The medial prefrontal cortex (mPFC) has been considered to participate in many higher cognitive functions, such as memory formation and spatial navigation. These cognitive functions are modulated by cholinergic afferents via muscarinic acetylcholine receptors. Previous pharmacological studies have strongly suggested that the M1 receptor (M1R) is the most important subtype among muscarinic receptors to perform these cognitive functions. Actually, M1R is abundant in mPFC. However, the proportion of somata containing M1R among cortical cellular types, and the precise intracellular localization of M1R remain unclear. In this study, to clarify the precise immunolocalization of M1R in rat mPFC, we examined three major cellular types, pyramidal neurons, inhibitory neurons, and astrocytes. M1R immunopositivity signals were found in the majority of the somata of both pyramidal neurons and inhibitory neurons. In pyramidal neurons, strong M1R immunopositivity signals were usually found throughout their somata and dendrites including spines. On the other hand, the signal strength of M1R immunopositivity in the somata of inhibitory neurons significantly varied. Some neurons showed strong signals. Whereas about 40% of GAD67‐immunopositive neurons and 30% of parvalbumin‐immunopositive neurons (PV neurons) showed only weak signals. In PV neurons, M1R immunopositivity signals were preferentially distributed in somata. Furthermore, we found that many astrocytes showed substantial M1R immunopositivity signals. These signals were also mainly distributed in their somata. Thus, the distribution pattern of M1R markedly differs between cellular types. This difference might underlie the cholinergic modulation of higher cognitive functions subserved by mPFC. John Wiley and Sons Inc. 2018-02-28 2018-06-01 /pmc/articles/PMC5900831/ /pubmed/29424434 http://dx.doi.org/10.1002/cne.24409 Text en © 2018 The Authors The Journal of Comparative Neurology Published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Oda, Satoko
Tsuneoka, Yousuke
Yoshida, Sachine
Adachi‐Akahane, Satomi
Ito, Masanori
Kuroda, Masaru
Funato, Hiromasa
Immunolocalization of muscarinic M1 receptor in the rat medial prefrontal cortex
title Immunolocalization of muscarinic M1 receptor in the rat medial prefrontal cortex
title_full Immunolocalization of muscarinic M1 receptor in the rat medial prefrontal cortex
title_fullStr Immunolocalization of muscarinic M1 receptor in the rat medial prefrontal cortex
title_full_unstemmed Immunolocalization of muscarinic M1 receptor in the rat medial prefrontal cortex
title_short Immunolocalization of muscarinic M1 receptor in the rat medial prefrontal cortex
title_sort immunolocalization of muscarinic m1 receptor in the rat medial prefrontal cortex
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5900831/
https://www.ncbi.nlm.nih.gov/pubmed/29424434
http://dx.doi.org/10.1002/cne.24409
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