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Semicontinuous sophorolipid fermentation using a novel bioreactor with dual ventilation pipes and dual sieve‐plates coupled with a novel separation system
Sophorolipids (SLs) are biosurfactants with widespread applications. The yield and purity of SLs are two important factors to be considered during their commercial large‐scale production. Notably, SL accumulation causes an increase in viscosity, decrease in dissolved oxygen and product inhibition in...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5902327/ https://www.ncbi.nlm.nih.gov/pubmed/29235728 http://dx.doi.org/10.1111/1751-7915.13028 |
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author | Zhang, Yaguang Jia, Dan Sun, Wanqi Yang, Xue Zhang, Chuanbo Zhao, Fanglong Lu, Wenyu |
author_facet | Zhang, Yaguang Jia, Dan Sun, Wanqi Yang, Xue Zhang, Chuanbo Zhao, Fanglong Lu, Wenyu |
author_sort | Zhang, Yaguang |
collection | PubMed |
description | Sophorolipids (SLs) are biosurfactants with widespread applications. The yield and purity of SLs are two important factors to be considered during their commercial large‐scale production. Notably, SL accumulation causes an increase in viscosity, decrease in dissolved oxygen and product inhibition in the fermentation medium. This inhibits the further production and purification of SLs. This describes the development of a novel integrated system for SL production using Candida albicans O‐13‐1. Semicontinuous fermentation was performed using a novel bioreactor with dual ventilation pipes and dual sieve‐plates (DVDSB). SLs were separated and recovered using a newly designed two‐stage separation system. After SL recovery, the fermentation broth containing residual glucose and oleic acid was recycled back into the bioreactor. This novel approach considerably alleviated the problem of product inhibition and accelerated the rate of substrate utilization. Production of SLs achieved was 477 g l(−1), while their productivity was 1.59 g l(−1) h(−1). Purity of SLs improved by 23.3%, from 60% to 74%, using DVDSB with the separation system. The conversion rate of carbon source increased from 0.5 g g(−1) (in the batch fermentation) to 0.6 g g(−1). These results indicated that the integrated system could improve the efficiency of production and purity of SLs. |
format | Online Article Text |
id | pubmed-5902327 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-59023272018-04-23 Semicontinuous sophorolipid fermentation using a novel bioreactor with dual ventilation pipes and dual sieve‐plates coupled with a novel separation system Zhang, Yaguang Jia, Dan Sun, Wanqi Yang, Xue Zhang, Chuanbo Zhao, Fanglong Lu, Wenyu Microb Biotechnol Research Articles Sophorolipids (SLs) are biosurfactants with widespread applications. The yield and purity of SLs are two important factors to be considered during their commercial large‐scale production. Notably, SL accumulation causes an increase in viscosity, decrease in dissolved oxygen and product inhibition in the fermentation medium. This inhibits the further production and purification of SLs. This describes the development of a novel integrated system for SL production using Candida albicans O‐13‐1. Semicontinuous fermentation was performed using a novel bioreactor with dual ventilation pipes and dual sieve‐plates (DVDSB). SLs were separated and recovered using a newly designed two‐stage separation system. After SL recovery, the fermentation broth containing residual glucose and oleic acid was recycled back into the bioreactor. This novel approach considerably alleviated the problem of product inhibition and accelerated the rate of substrate utilization. Production of SLs achieved was 477 g l(−1), while their productivity was 1.59 g l(−1) h(−1). Purity of SLs improved by 23.3%, from 60% to 74%, using DVDSB with the separation system. The conversion rate of carbon source increased from 0.5 g g(−1) (in the batch fermentation) to 0.6 g g(−1). These results indicated that the integrated system could improve the efficiency of production and purity of SLs. John Wiley and Sons Inc. 2017-12-13 /pmc/articles/PMC5902327/ /pubmed/29235728 http://dx.doi.org/10.1111/1751-7915.13028 Text en © 2017 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Zhang, Yaguang Jia, Dan Sun, Wanqi Yang, Xue Zhang, Chuanbo Zhao, Fanglong Lu, Wenyu Semicontinuous sophorolipid fermentation using a novel bioreactor with dual ventilation pipes and dual sieve‐plates coupled with a novel separation system |
title | Semicontinuous sophorolipid fermentation using a novel bioreactor with dual ventilation pipes and dual sieve‐plates coupled with a novel separation system |
title_full | Semicontinuous sophorolipid fermentation using a novel bioreactor with dual ventilation pipes and dual sieve‐plates coupled with a novel separation system |
title_fullStr | Semicontinuous sophorolipid fermentation using a novel bioreactor with dual ventilation pipes and dual sieve‐plates coupled with a novel separation system |
title_full_unstemmed | Semicontinuous sophorolipid fermentation using a novel bioreactor with dual ventilation pipes and dual sieve‐plates coupled with a novel separation system |
title_short | Semicontinuous sophorolipid fermentation using a novel bioreactor with dual ventilation pipes and dual sieve‐plates coupled with a novel separation system |
title_sort | semicontinuous sophorolipid fermentation using a novel bioreactor with dual ventilation pipes and dual sieve‐plates coupled with a novel separation system |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5902327/ https://www.ncbi.nlm.nih.gov/pubmed/29235728 http://dx.doi.org/10.1111/1751-7915.13028 |
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