Pilot study of novel lab methodology and testing of platelet function in adolescent women with heavy menstrual bleeding

INTRODUCTION: Approximately 40% of adolescent women experience heavy menstrual bleeding (HMB), and 10–62% of them have an underlying bleeding disorder (BD). Diagnosing BD remains challenging due to limitations of available clinical platelet function assays. AIM: To characterize platelet function in a population of adolescent women with HMB using small-volume whole blood assays. METHODS: Anticoagulated whole blood was used to assess platelet GPIIbIIIa activation, α-granule secretion, and aggregation in response to multiple agonists. Platelet adhesion on collagen or von Willebrand Factor (VWF) under static and shear flow was also assessed. RESULTS: Fifteen participants with HMB were included in the study, of which eight were diagnosed with a clinically-identifiable BD. Platelet activation was blunted in response to calcium ionophore in participants without a BD diagnosis compared to all other participants. Impaired GPIIbIIIa activation was observed in response to all GPCR agonists, except ADP, in participants with qualitative platelet disorders. Our assays detected platelet aggregation in the majority of participants with a BD in response to ADP, CRP, TRAP-6, or U46619. Platelet adhesion and aggregation on collagen and VWF was decreased for participants with VWD. CONCLUSION: Participants with and without BD exhibited aberrant platelet function in several assays in response to select agonists.