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Stringent Response and AggR-Dependent Virulence Regulation in the Enteroaggregative Escherichia coli Strain 042

Virulence expression in the enteroaggregative Escherichia coli strain 042 requires the transcriptional activator AggR. We show in this report that, as reported for other virulence factors, the nucleotide second messenger (p)ppGpp is needed for a high expression level of AggR. As expected from these...

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Autores principales: Hüttener, Mário, Prieto, Alejandro, Espelt, Joan, Bernabeu, Manuel, Juárez, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5902536/
https://www.ncbi.nlm.nih.gov/pubmed/29692772
http://dx.doi.org/10.3389/fmicb.2018.00717
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author Hüttener, Mário
Prieto, Alejandro
Espelt, Joan
Bernabeu, Manuel
Juárez, Antonio
author_facet Hüttener, Mário
Prieto, Alejandro
Espelt, Joan
Bernabeu, Manuel
Juárez, Antonio
author_sort Hüttener, Mário
collection PubMed
description Virulence expression in the enteroaggregative Escherichia coli strain 042 requires the transcriptional activator AggR. We show in this report that, as reported for other virulence factors, the nucleotide second messenger (p)ppGpp is needed for a high expression level of AggR. As expected from these findings, expression of AggR-activated genes such as the AafA pilin subunit is downregulated in the absence of (p)ppGpp. Considering the fact that biofilm formation in strain 042 requires the AafA protein, biofilm development in strain 042 is impaired in derivatives that lack either the AggR protein, the virulence plasmid that encodes AggR (pAA2) or the ability to synthesize (p)ppGpp. These results show a direct correlation between (p)ppGpp, expression of AggR and biofilm development in strain 042.
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spelling pubmed-59025362018-04-24 Stringent Response and AggR-Dependent Virulence Regulation in the Enteroaggregative Escherichia coli Strain 042 Hüttener, Mário Prieto, Alejandro Espelt, Joan Bernabeu, Manuel Juárez, Antonio Front Microbiol Microbiology Virulence expression in the enteroaggregative Escherichia coli strain 042 requires the transcriptional activator AggR. We show in this report that, as reported for other virulence factors, the nucleotide second messenger (p)ppGpp is needed for a high expression level of AggR. As expected from these findings, expression of AggR-activated genes such as the AafA pilin subunit is downregulated in the absence of (p)ppGpp. Considering the fact that biofilm formation in strain 042 requires the AafA protein, biofilm development in strain 042 is impaired in derivatives that lack either the AggR protein, the virulence plasmid that encodes AggR (pAA2) or the ability to synthesize (p)ppGpp. These results show a direct correlation between (p)ppGpp, expression of AggR and biofilm development in strain 042. Frontiers Media S.A. 2018-04-10 /pmc/articles/PMC5902536/ /pubmed/29692772 http://dx.doi.org/10.3389/fmicb.2018.00717 Text en Copyright © 2018 Hüttener, Prieto, Espelt, Bernabeu and Juárez. http://creativecommons.org/licenses/by/4.0/ This is an openaccess article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Hüttener, Mário
Prieto, Alejandro
Espelt, Joan
Bernabeu, Manuel
Juárez, Antonio
Stringent Response and AggR-Dependent Virulence Regulation in the Enteroaggregative Escherichia coli Strain 042
title Stringent Response and AggR-Dependent Virulence Regulation in the Enteroaggregative Escherichia coli Strain 042
title_full Stringent Response and AggR-Dependent Virulence Regulation in the Enteroaggregative Escherichia coli Strain 042
title_fullStr Stringent Response and AggR-Dependent Virulence Regulation in the Enteroaggregative Escherichia coli Strain 042
title_full_unstemmed Stringent Response and AggR-Dependent Virulence Regulation in the Enteroaggregative Escherichia coli Strain 042
title_short Stringent Response and AggR-Dependent Virulence Regulation in the Enteroaggregative Escherichia coli Strain 042
title_sort stringent response and aggr-dependent virulence regulation in the enteroaggregative escherichia coli strain 042
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5902536/
https://www.ncbi.nlm.nih.gov/pubmed/29692772
http://dx.doi.org/10.3389/fmicb.2018.00717
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