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Stringent Response and AggR-Dependent Virulence Regulation in the Enteroaggregative Escherichia coli Strain 042
Virulence expression in the enteroaggregative Escherichia coli strain 042 requires the transcriptional activator AggR. We show in this report that, as reported for other virulence factors, the nucleotide second messenger (p)ppGpp is needed for a high expression level of AggR. As expected from these...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5902536/ https://www.ncbi.nlm.nih.gov/pubmed/29692772 http://dx.doi.org/10.3389/fmicb.2018.00717 |
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author | Hüttener, Mário Prieto, Alejandro Espelt, Joan Bernabeu, Manuel Juárez, Antonio |
author_facet | Hüttener, Mário Prieto, Alejandro Espelt, Joan Bernabeu, Manuel Juárez, Antonio |
author_sort | Hüttener, Mário |
collection | PubMed |
description | Virulence expression in the enteroaggregative Escherichia coli strain 042 requires the transcriptional activator AggR. We show in this report that, as reported for other virulence factors, the nucleotide second messenger (p)ppGpp is needed for a high expression level of AggR. As expected from these findings, expression of AggR-activated genes such as the AafA pilin subunit is downregulated in the absence of (p)ppGpp. Considering the fact that biofilm formation in strain 042 requires the AafA protein, biofilm development in strain 042 is impaired in derivatives that lack either the AggR protein, the virulence plasmid that encodes AggR (pAA2) or the ability to synthesize (p)ppGpp. These results show a direct correlation between (p)ppGpp, expression of AggR and biofilm development in strain 042. |
format | Online Article Text |
id | pubmed-5902536 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-59025362018-04-24 Stringent Response and AggR-Dependent Virulence Regulation in the Enteroaggregative Escherichia coli Strain 042 Hüttener, Mário Prieto, Alejandro Espelt, Joan Bernabeu, Manuel Juárez, Antonio Front Microbiol Microbiology Virulence expression in the enteroaggregative Escherichia coli strain 042 requires the transcriptional activator AggR. We show in this report that, as reported for other virulence factors, the nucleotide second messenger (p)ppGpp is needed for a high expression level of AggR. As expected from these findings, expression of AggR-activated genes such as the AafA pilin subunit is downregulated in the absence of (p)ppGpp. Considering the fact that biofilm formation in strain 042 requires the AafA protein, biofilm development in strain 042 is impaired in derivatives that lack either the AggR protein, the virulence plasmid that encodes AggR (pAA2) or the ability to synthesize (p)ppGpp. These results show a direct correlation between (p)ppGpp, expression of AggR and biofilm development in strain 042. Frontiers Media S.A. 2018-04-10 /pmc/articles/PMC5902536/ /pubmed/29692772 http://dx.doi.org/10.3389/fmicb.2018.00717 Text en Copyright © 2018 Hüttener, Prieto, Espelt, Bernabeu and Juárez. http://creativecommons.org/licenses/by/4.0/ This is an openaccess article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Hüttener, Mário Prieto, Alejandro Espelt, Joan Bernabeu, Manuel Juárez, Antonio Stringent Response and AggR-Dependent Virulence Regulation in the Enteroaggregative Escherichia coli Strain 042 |
title | Stringent Response and AggR-Dependent Virulence Regulation in the Enteroaggregative Escherichia coli Strain 042 |
title_full | Stringent Response and AggR-Dependent Virulence Regulation in the Enteroaggregative Escherichia coli Strain 042 |
title_fullStr | Stringent Response and AggR-Dependent Virulence Regulation in the Enteroaggregative Escherichia coli Strain 042 |
title_full_unstemmed | Stringent Response and AggR-Dependent Virulence Regulation in the Enteroaggregative Escherichia coli Strain 042 |
title_short | Stringent Response and AggR-Dependent Virulence Regulation in the Enteroaggregative Escherichia coli Strain 042 |
title_sort | stringent response and aggr-dependent virulence regulation in the enteroaggregative escherichia coli strain 042 |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5902536/ https://www.ncbi.nlm.nih.gov/pubmed/29692772 http://dx.doi.org/10.3389/fmicb.2018.00717 |
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