Hsp90 Interacts With Tm-2(2) and Is Essential for Tm-2(2)-Mediated Resistance to Tobacco mosaic virus

The tomato resistance gene Tm-2(2) encodes a coiled coil-nucleotide binding site-leucine rich repeat type resistance protein and confers effective immune response against tobamoviruses by detecting the presence of viral movement proteins (MPs). In this study, we show that the Nicotiana benthamiana H...

Descripción completa

Detalles Bibliográficos
Autores principales: Qian, Lichao, Zhao, Jinping, Du, Yumei, Zhao, Xijuan, Han, Meng, Liu, Yule
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5902563/
https://www.ncbi.nlm.nih.gov/pubmed/29692788
http://dx.doi.org/10.3389/fpls.2018.00411
_version_ 1783314779268448256
author Qian, Lichao
Zhao, Jinping
Du, Yumei
Zhao, Xijuan
Han, Meng
Liu, Yule
author_facet Qian, Lichao
Zhao, Jinping
Du, Yumei
Zhao, Xijuan
Han, Meng
Liu, Yule
author_sort Qian, Lichao
collection PubMed
description The tomato resistance gene Tm-2(2) encodes a coiled coil-nucleotide binding site-leucine rich repeat type resistance protein and confers effective immune response against tobamoviruses by detecting the presence of viral movement proteins (MPs). In this study, we show that the Nicotiana benthamiana Heat shock protein 90-kD (Hsp90) interacts with Tm-2(2). Silencing of Hsp90 reduced Tm-2(2)-mediated resistance to Tobacco mosaic virus (TMV) and the steady-state levels of Tm-2(2) protein. Further, Hsp90 associates with SGT1 in yeast and in plant cells. These results suggest that Hsp90-SGT1 complex takes part in Tm-2(2)-mediated TMV resistance by functioning as chaperone to regulate Tm-2(2) stability.
format Online
Article
Text
id pubmed-5902563
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-59025632018-04-24 Hsp90 Interacts With Tm-2(2) and Is Essential for Tm-2(2)-Mediated Resistance to Tobacco mosaic virus Qian, Lichao Zhao, Jinping Du, Yumei Zhao, Xijuan Han, Meng Liu, Yule Front Plant Sci Plant Science The tomato resistance gene Tm-2(2) encodes a coiled coil-nucleotide binding site-leucine rich repeat type resistance protein and confers effective immune response against tobamoviruses by detecting the presence of viral movement proteins (MPs). In this study, we show that the Nicotiana benthamiana Heat shock protein 90-kD (Hsp90) interacts with Tm-2(2). Silencing of Hsp90 reduced Tm-2(2)-mediated resistance to Tobacco mosaic virus (TMV) and the steady-state levels of Tm-2(2) protein. Further, Hsp90 associates with SGT1 in yeast and in plant cells. These results suggest that Hsp90-SGT1 complex takes part in Tm-2(2)-mediated TMV resistance by functioning as chaperone to regulate Tm-2(2) stability. Frontiers Media S.A. 2018-04-10 /pmc/articles/PMC5902563/ /pubmed/29692788 http://dx.doi.org/10.3389/fpls.2018.00411 Text en Copyright © 2018 Qian, Zhao, Du, Zhao, Han and Liu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Qian, Lichao
Zhao, Jinping
Du, Yumei
Zhao, Xijuan
Han, Meng
Liu, Yule
Hsp90 Interacts With Tm-2(2) and Is Essential for Tm-2(2)-Mediated Resistance to Tobacco mosaic virus
title Hsp90 Interacts With Tm-2(2) and Is Essential for Tm-2(2)-Mediated Resistance to Tobacco mosaic virus
title_full Hsp90 Interacts With Tm-2(2) and Is Essential for Tm-2(2)-Mediated Resistance to Tobacco mosaic virus
title_fullStr Hsp90 Interacts With Tm-2(2) and Is Essential for Tm-2(2)-Mediated Resistance to Tobacco mosaic virus
title_full_unstemmed Hsp90 Interacts With Tm-2(2) and Is Essential for Tm-2(2)-Mediated Resistance to Tobacco mosaic virus
title_short Hsp90 Interacts With Tm-2(2) and Is Essential for Tm-2(2)-Mediated Resistance to Tobacco mosaic virus
title_sort hsp90 interacts with tm-2(2) and is essential for tm-2(2)-mediated resistance to tobacco mosaic virus
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5902563/
https://www.ncbi.nlm.nih.gov/pubmed/29692788
http://dx.doi.org/10.3389/fpls.2018.00411
work_keys_str_mv AT qianlichao hsp90interactswithtm22andisessentialfortm22mediatedresistancetotobaccomosaicvirus
AT zhaojinping hsp90interactswithtm22andisessentialfortm22mediatedresistancetotobaccomosaicvirus
AT duyumei hsp90interactswithtm22andisessentialfortm22mediatedresistancetotobaccomosaicvirus
AT zhaoxijuan hsp90interactswithtm22andisessentialfortm22mediatedresistancetotobaccomosaicvirus
AT hanmeng hsp90interactswithtm22andisessentialfortm22mediatedresistancetotobaccomosaicvirus
AT liuyule hsp90interactswithtm22andisessentialfortm22mediatedresistancetotobaccomosaicvirus

Ejemplares similares