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Systemic and sex-biased regulation of OBP expression under semiochemical stimuli

Constitutive expression of Odorant-Binding Proteins (OBPs) in antennae and other body parts has been examined mainly to infer their involvement in insect olfaction, while their regulation in response to semiochemical stimuli has remained poorly known. Previous studies of semiochemical response were...

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Autores principales: Paula, Débora Pires, Togawa, Roberto Coiti, do Carmo Costa, Marcos Mota, Grynberg, Priscila, Martins, Natália Florêncio, Andow, David Alan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5902564/
https://www.ncbi.nlm.nih.gov/pubmed/29662070
http://dx.doi.org/10.1038/s41598-018-24297-z
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author Paula, Débora Pires
Togawa, Roberto Coiti
do Carmo Costa, Marcos Mota
Grynberg, Priscila
Martins, Natália Florêncio
Andow, David Alan
author_facet Paula, Débora Pires
Togawa, Roberto Coiti
do Carmo Costa, Marcos Mota
Grynberg, Priscila
Martins, Natália Florêncio
Andow, David Alan
author_sort Paula, Débora Pires
collection PubMed
description Constitutive expression of Odorant-Binding Proteins (OBPs) in antennae and other body parts has been examined mainly to infer their involvement in insect olfaction, while their regulation in response to semiochemical stimuli has remained poorly known. Previous studies of semiochemical response were basically done using electrophysiology, which integrates the response of the set of OBPs present in an antenna or sensillum, without revealing the regulation of OBPs or which ones might be involved. In this study we used boll weevil as a model and mined its OBPs by RNA-Seq to study their simultaneous antennal expression by qPCR under controlled semiochemical stimuli with aggregation pheromone and plant volatiles. In the absence of a semiochemical stimulus, 23 of 24 OBPs were constitutively expressed in the antenna in both sexes. Semiochemicals changed systemically the expression of OBPs in both sexes. There were different patterns of up- and down-regulation in female antennae for each semiochemical stimulus, consistent with female chemical ecology. On the other hand, the only response in males was down-regulation of some OBPs. We suggest that these systemic changes in OBP expression might be related to enhancing detection of the semiochemical stimuli and/or priming the olfactory system to detect other environmental chemicals.
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spelling pubmed-59025642018-04-25 Systemic and sex-biased regulation of OBP expression under semiochemical stimuli Paula, Débora Pires Togawa, Roberto Coiti do Carmo Costa, Marcos Mota Grynberg, Priscila Martins, Natália Florêncio Andow, David Alan Sci Rep Article Constitutive expression of Odorant-Binding Proteins (OBPs) in antennae and other body parts has been examined mainly to infer their involvement in insect olfaction, while their regulation in response to semiochemical stimuli has remained poorly known. Previous studies of semiochemical response were basically done using electrophysiology, which integrates the response of the set of OBPs present in an antenna or sensillum, without revealing the regulation of OBPs or which ones might be involved. In this study we used boll weevil as a model and mined its OBPs by RNA-Seq to study their simultaneous antennal expression by qPCR under controlled semiochemical stimuli with aggregation pheromone and plant volatiles. In the absence of a semiochemical stimulus, 23 of 24 OBPs were constitutively expressed in the antenna in both sexes. Semiochemicals changed systemically the expression of OBPs in both sexes. There were different patterns of up- and down-regulation in female antennae for each semiochemical stimulus, consistent with female chemical ecology. On the other hand, the only response in males was down-regulation of some OBPs. We suggest that these systemic changes in OBP expression might be related to enhancing detection of the semiochemical stimuli and/or priming the olfactory system to detect other environmental chemicals. Nature Publishing Group UK 2018-04-16 /pmc/articles/PMC5902564/ /pubmed/29662070 http://dx.doi.org/10.1038/s41598-018-24297-z Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Paula, Débora Pires
Togawa, Roberto Coiti
do Carmo Costa, Marcos Mota
Grynberg, Priscila
Martins, Natália Florêncio
Andow, David Alan
Systemic and sex-biased regulation of OBP expression under semiochemical stimuli
title Systemic and sex-biased regulation of OBP expression under semiochemical stimuli
title_full Systemic and sex-biased regulation of OBP expression under semiochemical stimuli
title_fullStr Systemic and sex-biased regulation of OBP expression under semiochemical stimuli
title_full_unstemmed Systemic and sex-biased regulation of OBP expression under semiochemical stimuli
title_short Systemic and sex-biased regulation of OBP expression under semiochemical stimuli
title_sort systemic and sex-biased regulation of obp expression under semiochemical stimuli
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5902564/
https://www.ncbi.nlm.nih.gov/pubmed/29662070
http://dx.doi.org/10.1038/s41598-018-24297-z
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