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Genotyping of Plasmodiophora brassicae reveals the presence of distinct populations

BACKGROUND: Plasmodiophora brassicae is a soilborne pathogen of the family Brassicaceae and the causal agent of clubroot disease. In Canada, P. brassicae is now one of the most important constraints to canola (Brassica napus) production, and is managed mainly by the deployment of resistant cultivars...

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Autores principales: Holtz, Michael D., Hwang, Sheau-Fang, Strelkov, Stephen E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5902848/
https://www.ncbi.nlm.nih.gov/pubmed/29661147
http://dx.doi.org/10.1186/s12864-018-4658-1
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author Holtz, Michael D.
Hwang, Sheau-Fang
Strelkov, Stephen E.
author_facet Holtz, Michael D.
Hwang, Sheau-Fang
Strelkov, Stephen E.
author_sort Holtz, Michael D.
collection PubMed
description BACKGROUND: Plasmodiophora brassicae is a soilborne pathogen of the family Brassicaceae and the causal agent of clubroot disease. In Canada, P. brassicae is now one of the most important constraints to canola (Brassica napus) production, and is managed mainly by the deployment of resistant cultivars. In recent years, however, new strains of the pathogen have emerged that are capable of overcoming host resistance, posing new challenges for disease management. Despite its economic significance, molecular studies of P. brassicae are rare, mainly because this microorganism cannot be cultured outside of its host. RESULTS: Restriction site-associated DNA sequencing (RADseq) was used to examine the genetic diversity within P. brassicae single-spore and field isolates collected from across Canada. The isolates included individuals that were either capable or incapable of causing disease on clubroot resistant canola cultivars. Over 8750 variants were identified through RADseq. Population analysis indicated that most isolates belonged to one of two distinct populations, corresponding with the ability of isolates to cause disease on resistant cultivars. Within each population, there were low levels of genetic diversity. One thousand and fifty of the genetic variants that distinguished the two populations were nonsynonymous, altering the coding sequences of genes. CONCLUSION: The application of RADseq revealed two distinct populations of P. brassicae in Canada, suggesting multiple introductions of the pathogen into the country. The genetic variation found here will be important for future research and monitoring of the pathogen. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-018-4658-1) contains supplementary material, which is available to authorized users.
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spelling pubmed-59028482018-04-23 Genotyping of Plasmodiophora brassicae reveals the presence of distinct populations Holtz, Michael D. Hwang, Sheau-Fang Strelkov, Stephen E. BMC Genomics Research Article BACKGROUND: Plasmodiophora brassicae is a soilborne pathogen of the family Brassicaceae and the causal agent of clubroot disease. In Canada, P. brassicae is now one of the most important constraints to canola (Brassica napus) production, and is managed mainly by the deployment of resistant cultivars. In recent years, however, new strains of the pathogen have emerged that are capable of overcoming host resistance, posing new challenges for disease management. Despite its economic significance, molecular studies of P. brassicae are rare, mainly because this microorganism cannot be cultured outside of its host. RESULTS: Restriction site-associated DNA sequencing (RADseq) was used to examine the genetic diversity within P. brassicae single-spore and field isolates collected from across Canada. The isolates included individuals that were either capable or incapable of causing disease on clubroot resistant canola cultivars. Over 8750 variants were identified through RADseq. Population analysis indicated that most isolates belonged to one of two distinct populations, corresponding with the ability of isolates to cause disease on resistant cultivars. Within each population, there were low levels of genetic diversity. One thousand and fifty of the genetic variants that distinguished the two populations were nonsynonymous, altering the coding sequences of genes. CONCLUSION: The application of RADseq revealed two distinct populations of P. brassicae in Canada, suggesting multiple introductions of the pathogen into the country. The genetic variation found here will be important for future research and monitoring of the pathogen. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-018-4658-1) contains supplementary material, which is available to authorized users. BioMed Central 2018-04-16 /pmc/articles/PMC5902848/ /pubmed/29661147 http://dx.doi.org/10.1186/s12864-018-4658-1 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Holtz, Michael D.
Hwang, Sheau-Fang
Strelkov, Stephen E.
Genotyping of Plasmodiophora brassicae reveals the presence of distinct populations
title Genotyping of Plasmodiophora brassicae reveals the presence of distinct populations
title_full Genotyping of Plasmodiophora brassicae reveals the presence of distinct populations
title_fullStr Genotyping of Plasmodiophora brassicae reveals the presence of distinct populations
title_full_unstemmed Genotyping of Plasmodiophora brassicae reveals the presence of distinct populations
title_short Genotyping of Plasmodiophora brassicae reveals the presence of distinct populations
title_sort genotyping of plasmodiophora brassicae reveals the presence of distinct populations
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5902848/
https://www.ncbi.nlm.nih.gov/pubmed/29661147
http://dx.doi.org/10.1186/s12864-018-4658-1
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