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Aerobic Training Prevents Heatstrokes in Calsequestrin-1 Knockout Mice by Reducing Oxidative Stress

Calsequestrin-1 knockout (CASQ1-null) mice suffer lethal episodes when exposed to strenuous exercise and environmental heat, crises known as exertional/environmental heatstroke (EHS). We previously demonstrated that administration of exogenous antioxidants (N-acetylcysteine and trolox) reduces CASQ1...

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Autores principales: Guarnier, Flávia Alessandra, Michelucci, Antonio, Serano, Matteo, Pietrangelo, Laura, Pecorai, Claudia, Boncompagni, Simona, Protasi, Feliciano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5903204/
https://www.ncbi.nlm.nih.gov/pubmed/29849896
http://dx.doi.org/10.1155/2018/4652480
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author Guarnier, Flávia Alessandra
Michelucci, Antonio
Serano, Matteo
Pietrangelo, Laura
Pecorai, Claudia
Boncompagni, Simona
Protasi, Feliciano
author_facet Guarnier, Flávia Alessandra
Michelucci, Antonio
Serano, Matteo
Pietrangelo, Laura
Pecorai, Claudia
Boncompagni, Simona
Protasi, Feliciano
author_sort Guarnier, Flávia Alessandra
collection PubMed
description Calsequestrin-1 knockout (CASQ1-null) mice suffer lethal episodes when exposed to strenuous exercise and environmental heat, crises known as exertional/environmental heatstroke (EHS). We previously demonstrated that administration of exogenous antioxidants (N-acetylcysteine and trolox) reduces CASQ1-null mortality during exposure to heat. As aerobic training is known to boost endogenous antioxidant protection, we subjected CASQ1-null mice to treadmill running for 2 months at 60% of their maximal speed for 1 h, 5 times/week. When exposed to heat stress protocol (41°C/1 h), the mortality rate of CASQ1-null mice was significantly reduced compared to untrained animals (86% versus 16%). Protection from heatstrokes was accompanied by a reduced increase in core temperature during the stress protocol and by an increased threshold of response to caffeine of isolated extensor digitorum longus muscles during in vitro contracture test. At cellular and molecular levels, aerobic training (i) improved mitochondrial function while reducing their damage and (ii) lowered calpain activity and lipid peroxidation in membranes isolated from sarcoplasmic reticulum and mitochondria. Based on this evidence, we hypothesize that the protective effect of aerobic training is essentially mediated by a reduction in oxidative stress during exposure of CASQ1-null mice to adverse environmental conditions.
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spelling pubmed-59032042018-05-30 Aerobic Training Prevents Heatstrokes in Calsequestrin-1 Knockout Mice by Reducing Oxidative Stress Guarnier, Flávia Alessandra Michelucci, Antonio Serano, Matteo Pietrangelo, Laura Pecorai, Claudia Boncompagni, Simona Protasi, Feliciano Oxid Med Cell Longev Research Article Calsequestrin-1 knockout (CASQ1-null) mice suffer lethal episodes when exposed to strenuous exercise and environmental heat, crises known as exertional/environmental heatstroke (EHS). We previously demonstrated that administration of exogenous antioxidants (N-acetylcysteine and trolox) reduces CASQ1-null mortality during exposure to heat. As aerobic training is known to boost endogenous antioxidant protection, we subjected CASQ1-null mice to treadmill running for 2 months at 60% of their maximal speed for 1 h, 5 times/week. When exposed to heat stress protocol (41°C/1 h), the mortality rate of CASQ1-null mice was significantly reduced compared to untrained animals (86% versus 16%). Protection from heatstrokes was accompanied by a reduced increase in core temperature during the stress protocol and by an increased threshold of response to caffeine of isolated extensor digitorum longus muscles during in vitro contracture test. At cellular and molecular levels, aerobic training (i) improved mitochondrial function while reducing their damage and (ii) lowered calpain activity and lipid peroxidation in membranes isolated from sarcoplasmic reticulum and mitochondria. Based on this evidence, we hypothesize that the protective effect of aerobic training is essentially mediated by a reduction in oxidative stress during exposure of CASQ1-null mice to adverse environmental conditions. Hindawi 2018-04-03 /pmc/articles/PMC5903204/ /pubmed/29849896 http://dx.doi.org/10.1155/2018/4652480 Text en Copyright © 2018 Flávia Alessandra Guarnier et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Guarnier, Flávia Alessandra
Michelucci, Antonio
Serano, Matteo
Pietrangelo, Laura
Pecorai, Claudia
Boncompagni, Simona
Protasi, Feliciano
Aerobic Training Prevents Heatstrokes in Calsequestrin-1 Knockout Mice by Reducing Oxidative Stress
title Aerobic Training Prevents Heatstrokes in Calsequestrin-1 Knockout Mice by Reducing Oxidative Stress
title_full Aerobic Training Prevents Heatstrokes in Calsequestrin-1 Knockout Mice by Reducing Oxidative Stress
title_fullStr Aerobic Training Prevents Heatstrokes in Calsequestrin-1 Knockout Mice by Reducing Oxidative Stress
title_full_unstemmed Aerobic Training Prevents Heatstrokes in Calsequestrin-1 Knockout Mice by Reducing Oxidative Stress
title_short Aerobic Training Prevents Heatstrokes in Calsequestrin-1 Knockout Mice by Reducing Oxidative Stress
title_sort aerobic training prevents heatstrokes in calsequestrin-1 knockout mice by reducing oxidative stress
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5903204/
https://www.ncbi.nlm.nih.gov/pubmed/29849896
http://dx.doi.org/10.1155/2018/4652480
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