Cargando…

Neural Stem Cell-Conditioned Medium Ameliorated Cerebral Ischemia-Reperfusion Injury in Rats

INTRODUCTION: Our previous study suggested that NSC-CM (neural stem cell-conditioned medium) inhibited cell apoptosis in vitro. In addition, many studies have shown that neurotrophic factors and microparticles secreted into a conditioned medium by NSCs had neuroprotective effects. Thus, we hypothesi...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, HongNa, Wang, Cuilan, Chen, Hui, Li, Lan, Ma, Shuang, Wang, Hao, Fu, YaRu, Qu, Tingyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5903322/
https://www.ncbi.nlm.nih.gov/pubmed/29765412
http://dx.doi.org/10.1155/2018/4659159
_version_ 1783314921989079040
author Yang, HongNa
Wang, Cuilan
Chen, Hui
Li, Lan
Ma, Shuang
Wang, Hao
Fu, YaRu
Qu, Tingyu
author_facet Yang, HongNa
Wang, Cuilan
Chen, Hui
Li, Lan
Ma, Shuang
Wang, Hao
Fu, YaRu
Qu, Tingyu
author_sort Yang, HongNa
collection PubMed
description INTRODUCTION: Our previous study suggested that NSC-CM (neural stem cell-conditioned medium) inhibited cell apoptosis in vitro. In addition, many studies have shown that neurotrophic factors and microparticles secreted into a conditioned medium by NSCs had neuroprotective effects. Thus, we hypothesized that NSC-CM had the capacity of protecting against cerebral I/R injury. METHODS: Adult male Sprague-Dawley rats receiving middle cerebral artery occlusion surgery as an animal model of cerebral I/R injury were randomly assigned to two groups: the control group and NSC-CM-treated group. 1.5 ml NSC-CM or PBS (phosphate buffer saline) was administrated slowly by tail vein at 3 h, 24 h, and 48 h after ischemia onset. RESULTS: NSC-CM significantly ameliorated neurological defects and reduced cerebral infarct volume, accompanied by preserved mitochondrial ultrastructure. In addition, we also found that NSC-CM significantly inhibited cell apoptosis in the ischemic hemisphere via improving the expression of Bcl-2 (B-cell lymphoma-2). CONCLUSION: NSC-CM might be an alternative and effective therapeutic intervention for ischemic stroke.
format Online
Article
Text
id pubmed-5903322
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-59033222018-05-14 Neural Stem Cell-Conditioned Medium Ameliorated Cerebral Ischemia-Reperfusion Injury in Rats Yang, HongNa Wang, Cuilan Chen, Hui Li, Lan Ma, Shuang Wang, Hao Fu, YaRu Qu, Tingyu Stem Cells Int Research Article INTRODUCTION: Our previous study suggested that NSC-CM (neural stem cell-conditioned medium) inhibited cell apoptosis in vitro. In addition, many studies have shown that neurotrophic factors and microparticles secreted into a conditioned medium by NSCs had neuroprotective effects. Thus, we hypothesized that NSC-CM had the capacity of protecting against cerebral I/R injury. METHODS: Adult male Sprague-Dawley rats receiving middle cerebral artery occlusion surgery as an animal model of cerebral I/R injury were randomly assigned to two groups: the control group and NSC-CM-treated group. 1.5 ml NSC-CM or PBS (phosphate buffer saline) was administrated slowly by tail vein at 3 h, 24 h, and 48 h after ischemia onset. RESULTS: NSC-CM significantly ameliorated neurological defects and reduced cerebral infarct volume, accompanied by preserved mitochondrial ultrastructure. In addition, we also found that NSC-CM significantly inhibited cell apoptosis in the ischemic hemisphere via improving the expression of Bcl-2 (B-cell lymphoma-2). CONCLUSION: NSC-CM might be an alternative and effective therapeutic intervention for ischemic stroke. Hindawi 2018-04-03 /pmc/articles/PMC5903322/ /pubmed/29765412 http://dx.doi.org/10.1155/2018/4659159 Text en Copyright © 2018 HongNa Yang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yang, HongNa
Wang, Cuilan
Chen, Hui
Li, Lan
Ma, Shuang
Wang, Hao
Fu, YaRu
Qu, Tingyu
Neural Stem Cell-Conditioned Medium Ameliorated Cerebral Ischemia-Reperfusion Injury in Rats
title Neural Stem Cell-Conditioned Medium Ameliorated Cerebral Ischemia-Reperfusion Injury in Rats
title_full Neural Stem Cell-Conditioned Medium Ameliorated Cerebral Ischemia-Reperfusion Injury in Rats
title_fullStr Neural Stem Cell-Conditioned Medium Ameliorated Cerebral Ischemia-Reperfusion Injury in Rats
title_full_unstemmed Neural Stem Cell-Conditioned Medium Ameliorated Cerebral Ischemia-Reperfusion Injury in Rats
title_short Neural Stem Cell-Conditioned Medium Ameliorated Cerebral Ischemia-Reperfusion Injury in Rats
title_sort neural stem cell-conditioned medium ameliorated cerebral ischemia-reperfusion injury in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5903322/
https://www.ncbi.nlm.nih.gov/pubmed/29765412
http://dx.doi.org/10.1155/2018/4659159
work_keys_str_mv AT yanghongna neuralstemcellconditionedmediumamelioratedcerebralischemiareperfusioninjuryinrats
AT wangcuilan neuralstemcellconditionedmediumamelioratedcerebralischemiareperfusioninjuryinrats
AT chenhui neuralstemcellconditionedmediumamelioratedcerebralischemiareperfusioninjuryinrats
AT lilan neuralstemcellconditionedmediumamelioratedcerebralischemiareperfusioninjuryinrats
AT mashuang neuralstemcellconditionedmediumamelioratedcerebralischemiareperfusioninjuryinrats
AT wanghao neuralstemcellconditionedmediumamelioratedcerebralischemiareperfusioninjuryinrats
AT fuyaru neuralstemcellconditionedmediumamelioratedcerebralischemiareperfusioninjuryinrats
AT qutingyu neuralstemcellconditionedmediumamelioratedcerebralischemiareperfusioninjuryinrats