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Actin and myosin II modulate differentiation of pluripotent stem cells
Use of stem cell-based therapies in tissue engineering and regenerative medicine is hindered by efficient means of directed differentiation. For pluripotent stem cells, an initial critical differentiation event is specification to one of three germ lineages: endoderm, mesoderm, and ectoderm. Differe...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5903644/ https://www.ncbi.nlm.nih.gov/pubmed/29664925 http://dx.doi.org/10.1371/journal.pone.0195588 |
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author | Boraas, Liana C. Pineda, Emma T. Ahsan, Tabassum |
author_facet | Boraas, Liana C. Pineda, Emma T. Ahsan, Tabassum |
author_sort | Boraas, Liana C. |
collection | PubMed |
description | Use of stem cell-based therapies in tissue engineering and regenerative medicine is hindered by efficient means of directed differentiation. For pluripotent stem cells, an initial critical differentiation event is specification to one of three germ lineages: endoderm, mesoderm, and ectoderm. Differentiation is known to be regulated by numerous extracellular and intracellular factors, but the role of the cytoskeleton during specification, or early differentiation, is still unknown. In these studies, we used agonists and antagonists to modulate actin polymerization and the actin-myosin molecular motor during spontaneous differentiation of embryonic stem cells in embryoid bodies. We found that inhibiting either actin polymerization or actin-myosin interactions led to a decrease in differentiation to the mesodermal lineage and an increase in differentiation to the endodermal lineage. Thus, targeting processes that regulate cytoskeletal tension may be effective in enhancing or inhibiting differentiation towards cells of the endodermal or mesodermal lineages, which include hepatocytes, islets, cardiomyocytes, endothelial cells, and osteocytes. Therefore, these fundamental findings demonstrate that modulation of the cytoskeleton may be useful in production for a range of cell-based therapies, including for liver, pancreatic, cardiac, vascular, and orthopedic applications. |
format | Online Article Text |
id | pubmed-5903644 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-59036442018-04-27 Actin and myosin II modulate differentiation of pluripotent stem cells Boraas, Liana C. Pineda, Emma T. Ahsan, Tabassum PLoS One Research Article Use of stem cell-based therapies in tissue engineering and regenerative medicine is hindered by efficient means of directed differentiation. For pluripotent stem cells, an initial critical differentiation event is specification to one of three germ lineages: endoderm, mesoderm, and ectoderm. Differentiation is known to be regulated by numerous extracellular and intracellular factors, but the role of the cytoskeleton during specification, or early differentiation, is still unknown. In these studies, we used agonists and antagonists to modulate actin polymerization and the actin-myosin molecular motor during spontaneous differentiation of embryonic stem cells in embryoid bodies. We found that inhibiting either actin polymerization or actin-myosin interactions led to a decrease in differentiation to the mesodermal lineage and an increase in differentiation to the endodermal lineage. Thus, targeting processes that regulate cytoskeletal tension may be effective in enhancing or inhibiting differentiation towards cells of the endodermal or mesodermal lineages, which include hepatocytes, islets, cardiomyocytes, endothelial cells, and osteocytes. Therefore, these fundamental findings demonstrate that modulation of the cytoskeleton may be useful in production for a range of cell-based therapies, including for liver, pancreatic, cardiac, vascular, and orthopedic applications. Public Library of Science 2018-04-17 /pmc/articles/PMC5903644/ /pubmed/29664925 http://dx.doi.org/10.1371/journal.pone.0195588 Text en © 2018 Boraas et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Boraas, Liana C. Pineda, Emma T. Ahsan, Tabassum Actin and myosin II modulate differentiation of pluripotent stem cells |
title | Actin and myosin II modulate differentiation of pluripotent stem cells |
title_full | Actin and myosin II modulate differentiation of pluripotent stem cells |
title_fullStr | Actin and myosin II modulate differentiation of pluripotent stem cells |
title_full_unstemmed | Actin and myosin II modulate differentiation of pluripotent stem cells |
title_short | Actin and myosin II modulate differentiation of pluripotent stem cells |
title_sort | actin and myosin ii modulate differentiation of pluripotent stem cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5903644/ https://www.ncbi.nlm.nih.gov/pubmed/29664925 http://dx.doi.org/10.1371/journal.pone.0195588 |
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