The long non-coding RNA uc.4 influences cell differentiation through the TGF-beta signaling pathway

In a previous study, we screened thousands of long non-coding RNAs (lncRNAs) to assess their potential relationship with congenital heart disease (CHD). In this study, uc.4 attracted our attention because of its high level of evolutionary conservation and its antisense orientation to the CASZ1 gene,...

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Autores principales: Cheng, Zijie, Zhang, Qijun, Yin, Anwen, Feng, Mengwen, Li, Hua, Liu, Hailang, Li, Yun, Qian, Lingmei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2018
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5903826/
https://www.ncbi.nlm.nih.gov/pubmed/29504607
http://dx.doi.org/10.1038/emm.2017.278
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author Cheng, Zijie
Zhang, Qijun
Yin, Anwen
Feng, Mengwen
Li, Hua
Liu, Hailang
Li, Yun
Qian, Lingmei
author_facet Cheng, Zijie
Zhang, Qijun
Yin, Anwen
Feng, Mengwen
Li, Hua
Liu, Hailang
Li, Yun
Qian, Lingmei
author_sort Cheng, Zijie
collection PubMed
description In a previous study, we screened thousands of long non-coding RNAs (lncRNAs) to assess their potential relationship with congenital heart disease (CHD). In this study, uc.4 attracted our attention because of its high level of evolutionary conservation and its antisense orientation to the CASZ1 gene, which is vital for heart development. We explored the function of uc.4 in cells and in zebrafish, and describe a potential mechanism of action. P19 cells were used to investigate the function of uc.4. We studied the effect of uc.4 overexpression on heart development in zebrafish. The overexpression of uc.4 influenced cell differentiation by inhibiting the TGF-beta signaling pathway and suppressed heart development in zebrafish, resulting in cardiac malformation. Taken together, our findings show that uc.4 is involved in heart development, thus providing a potential therapeutic target for CHD.
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spelling pubmed-59038262018-04-19 The long non-coding RNA uc.4 influences cell differentiation through the TGF-beta signaling pathway Cheng, Zijie Zhang, Qijun Yin, Anwen Feng, Mengwen Li, Hua Liu, Hailang Li, Yun Qian, Lingmei Exp Mol Med Original Article In a previous study, we screened thousands of long non-coding RNAs (lncRNAs) to assess their potential relationship with congenital heart disease (CHD). In this study, uc.4 attracted our attention because of its high level of evolutionary conservation and its antisense orientation to the CASZ1 gene, which is vital for heart development. We explored the function of uc.4 in cells and in zebrafish, and describe a potential mechanism of action. P19 cells were used to investigate the function of uc.4. We studied the effect of uc.4 overexpression on heart development in zebrafish. The overexpression of uc.4 influenced cell differentiation by inhibiting the TGF-beta signaling pathway and suppressed heart development in zebrafish, resulting in cardiac malformation. Taken together, our findings show that uc.4 is involved in heart development, thus providing a potential therapeutic target for CHD. Nature Publishing Group 2018-02 2018-02-16 /pmc/articles/PMC5903826/ /pubmed/29504607 http://dx.doi.org/10.1038/emm.2017.278 Text en Copyright © 2018 The Author(s) http://creativecommons.org/licenses/by-nc-sa/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/
spellingShingle Original Article
Cheng, Zijie
Zhang, Qijun
Yin, Anwen
Feng, Mengwen
Li, Hua
Liu, Hailang
Li, Yun
Qian, Lingmei
The long non-coding RNA uc.4 influences cell differentiation through the TGF-beta signaling pathway
title The long non-coding RNA uc.4 influences cell differentiation through the TGF-beta signaling pathway
title_full The long non-coding RNA uc.4 influences cell differentiation through the TGF-beta signaling pathway
title_fullStr The long non-coding RNA uc.4 influences cell differentiation through the TGF-beta signaling pathway
title_full_unstemmed The long non-coding RNA uc.4 influences cell differentiation through the TGF-beta signaling pathway
title_short The long non-coding RNA uc.4 influences cell differentiation through the TGF-beta signaling pathway
title_sort long non-coding rna uc.4 influences cell differentiation through the tgf-beta signaling pathway
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5903826/
https://www.ncbi.nlm.nih.gov/pubmed/29504607
http://dx.doi.org/10.1038/emm.2017.278
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