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Stable transformation of Babesia bigemina and Babesia bovis using a single transfection plasmid

Babesia bigemina and Babesia bovis, are the two major causes of bovine babesiosis, a global neglected disease in need of improved methods of control. Here, we describe a shared method for the stable transfection of these two parasites using electroporation and blasticidin/blasticidin deaminase as a...

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Detalles Bibliográficos
Autores principales: Silva, Marta G., Knowles, Donald P., Mazuz, Monica L., Cooke, Brian M., Suarez, Carlos E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5904164/
https://www.ncbi.nlm.nih.gov/pubmed/29666434
http://dx.doi.org/10.1038/s41598-018-23010-4