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Distribution and clinical associations of integrating conjugative elements and cag pathogenicity islands of Helicobacter pylori in Indonesia
The clinical associations and correlations with other virulence factors such as cag pathogenicity island (PAI) of the Integrating Conjugative Elements Helicobacter pylori TFSS (ICEHptfs), a new type IV secretion system (TFSS) in H. pylori has not been described. Among 103 studied strains from Indone...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5904169/ https://www.ncbi.nlm.nih.gov/pubmed/29666390 http://dx.doi.org/10.1038/s41598-018-24406-y |
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author | Waskito, Langgeng Agung Miftahussurur, Muhammad Lusida, Maria Inge Syam, Ari Fahrial Suzuki, Rumiko Subsomwong, Phawinee Uchida, Tomohisa Hamdan, Muhammad Nasronudin Yamaoka, Yoshio |
author_facet | Waskito, Langgeng Agung Miftahussurur, Muhammad Lusida, Maria Inge Syam, Ari Fahrial Suzuki, Rumiko Subsomwong, Phawinee Uchida, Tomohisa Hamdan, Muhammad Nasronudin Yamaoka, Yoshio |
author_sort | Waskito, Langgeng Agung |
collection | PubMed |
description | The clinical associations and correlations with other virulence factors such as cag pathogenicity island (PAI) of the Integrating Conjugative Elements Helicobacter pylori TFSS (ICEHptfs), a new type IV secretion system (TFSS) in H. pylori has not been described. Among 103 studied strains from Indonesia, almost all strains (99.0%) contained cag PAI with more than half (55.8%) were intact cag PAI. Patients infected with intact cag PAI strains showed significantly higher antral activity, inflammation and atrophy as well as corporal inflammation than those with non-intact cag PAI strains, confirming the virulence of cag PAI. Over half of strains (53.8%) contained ICEHptfs, predominantly consisted of ICEHptfs3-tfs4a (42.8%) and ICEHptfs3 (16.3%). Although patients infected with ICEHptfs-positive strains had lower H. pylori density, those with the complete ICEHptfs4b strains tended to have higher antral activity than the negative one. In combination, patients infected with combination of intact cag PAI-ICEHptfs-positive strains had more severe inflammation than those with non-intact cag PAI-ICEHptfs-negative, suggesting a possibility of a mutual correlation between these TFSS(s). |
format | Online Article Text |
id | pubmed-5904169 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-59041692018-04-30 Distribution and clinical associations of integrating conjugative elements and cag pathogenicity islands of Helicobacter pylori in Indonesia Waskito, Langgeng Agung Miftahussurur, Muhammad Lusida, Maria Inge Syam, Ari Fahrial Suzuki, Rumiko Subsomwong, Phawinee Uchida, Tomohisa Hamdan, Muhammad Nasronudin Yamaoka, Yoshio Sci Rep Article The clinical associations and correlations with other virulence factors such as cag pathogenicity island (PAI) of the Integrating Conjugative Elements Helicobacter pylori TFSS (ICEHptfs), a new type IV secretion system (TFSS) in H. pylori has not been described. Among 103 studied strains from Indonesia, almost all strains (99.0%) contained cag PAI with more than half (55.8%) were intact cag PAI. Patients infected with intact cag PAI strains showed significantly higher antral activity, inflammation and atrophy as well as corporal inflammation than those with non-intact cag PAI strains, confirming the virulence of cag PAI. Over half of strains (53.8%) contained ICEHptfs, predominantly consisted of ICEHptfs3-tfs4a (42.8%) and ICEHptfs3 (16.3%). Although patients infected with ICEHptfs-positive strains had lower H. pylori density, those with the complete ICEHptfs4b strains tended to have higher antral activity than the negative one. In combination, patients infected with combination of intact cag PAI-ICEHptfs-positive strains had more severe inflammation than those with non-intact cag PAI-ICEHptfs-negative, suggesting a possibility of a mutual correlation between these TFSS(s). Nature Publishing Group UK 2018-04-17 /pmc/articles/PMC5904169/ /pubmed/29666390 http://dx.doi.org/10.1038/s41598-018-24406-y Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Waskito, Langgeng Agung Miftahussurur, Muhammad Lusida, Maria Inge Syam, Ari Fahrial Suzuki, Rumiko Subsomwong, Phawinee Uchida, Tomohisa Hamdan, Muhammad Nasronudin Yamaoka, Yoshio Distribution and clinical associations of integrating conjugative elements and cag pathogenicity islands of Helicobacter pylori in Indonesia |
title | Distribution and clinical associations of integrating conjugative elements and cag pathogenicity islands of Helicobacter pylori in Indonesia |
title_full | Distribution and clinical associations of integrating conjugative elements and cag pathogenicity islands of Helicobacter pylori in Indonesia |
title_fullStr | Distribution and clinical associations of integrating conjugative elements and cag pathogenicity islands of Helicobacter pylori in Indonesia |
title_full_unstemmed | Distribution and clinical associations of integrating conjugative elements and cag pathogenicity islands of Helicobacter pylori in Indonesia |
title_short | Distribution and clinical associations of integrating conjugative elements and cag pathogenicity islands of Helicobacter pylori in Indonesia |
title_sort | distribution and clinical associations of integrating conjugative elements and cag pathogenicity islands of helicobacter pylori in indonesia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5904169/ https://www.ncbi.nlm.nih.gov/pubmed/29666390 http://dx.doi.org/10.1038/s41598-018-24406-y |
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