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Distribution and clinical associations of integrating conjugative elements and cag pathogenicity islands of Helicobacter pylori in Indonesia

The clinical associations and correlations with other virulence factors such as cag pathogenicity island (PAI) of the Integrating Conjugative Elements Helicobacter pylori TFSS (ICEHptfs), a new type IV secretion system (TFSS) in H. pylori has not been described. Among 103 studied strains from Indone...

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Autores principales: Waskito, Langgeng Agung, Miftahussurur, Muhammad, Lusida, Maria Inge, Syam, Ari Fahrial, Suzuki, Rumiko, Subsomwong, Phawinee, Uchida, Tomohisa, Hamdan, Muhammad, Nasronudin, Yamaoka, Yoshio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5904169/
https://www.ncbi.nlm.nih.gov/pubmed/29666390
http://dx.doi.org/10.1038/s41598-018-24406-y
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author Waskito, Langgeng Agung
Miftahussurur, Muhammad
Lusida, Maria Inge
Syam, Ari Fahrial
Suzuki, Rumiko
Subsomwong, Phawinee
Uchida, Tomohisa
Hamdan, Muhammad
Nasronudin
Yamaoka, Yoshio
author_facet Waskito, Langgeng Agung
Miftahussurur, Muhammad
Lusida, Maria Inge
Syam, Ari Fahrial
Suzuki, Rumiko
Subsomwong, Phawinee
Uchida, Tomohisa
Hamdan, Muhammad
Nasronudin
Yamaoka, Yoshio
author_sort Waskito, Langgeng Agung
collection PubMed
description The clinical associations and correlations with other virulence factors such as cag pathogenicity island (PAI) of the Integrating Conjugative Elements Helicobacter pylori TFSS (ICEHptfs), a new type IV secretion system (TFSS) in H. pylori has not been described. Among 103 studied strains from Indonesia, almost all strains (99.0%) contained cag PAI with more than half (55.8%) were intact cag PAI. Patients infected with intact cag PAI strains showed significantly higher antral activity, inflammation and atrophy as well as corporal inflammation than those with non-intact cag PAI strains, confirming the virulence of cag PAI. Over half of strains (53.8%) contained ICEHptfs, predominantly consisted of ICEHptfs3-tfs4a (42.8%) and ICEHptfs3 (16.3%). Although patients infected with ICEHptfs-positive strains had lower H. pylori density, those with the complete ICEHptfs4b strains tended to have higher antral activity than the negative one. In combination, patients infected with combination of intact cag PAI-ICEHptfs-positive strains had more severe inflammation than those with non-intact cag PAI-ICEHptfs-negative, suggesting a possibility of a mutual correlation between these TFSS(s).
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spelling pubmed-59041692018-04-30 Distribution and clinical associations of integrating conjugative elements and cag pathogenicity islands of Helicobacter pylori in Indonesia Waskito, Langgeng Agung Miftahussurur, Muhammad Lusida, Maria Inge Syam, Ari Fahrial Suzuki, Rumiko Subsomwong, Phawinee Uchida, Tomohisa Hamdan, Muhammad Nasronudin Yamaoka, Yoshio Sci Rep Article The clinical associations and correlations with other virulence factors such as cag pathogenicity island (PAI) of the Integrating Conjugative Elements Helicobacter pylori TFSS (ICEHptfs), a new type IV secretion system (TFSS) in H. pylori has not been described. Among 103 studied strains from Indonesia, almost all strains (99.0%) contained cag PAI with more than half (55.8%) were intact cag PAI. Patients infected with intact cag PAI strains showed significantly higher antral activity, inflammation and atrophy as well as corporal inflammation than those with non-intact cag PAI strains, confirming the virulence of cag PAI. Over half of strains (53.8%) contained ICEHptfs, predominantly consisted of ICEHptfs3-tfs4a (42.8%) and ICEHptfs3 (16.3%). Although patients infected with ICEHptfs-positive strains had lower H. pylori density, those with the complete ICEHptfs4b strains tended to have higher antral activity than the negative one. In combination, patients infected with combination of intact cag PAI-ICEHptfs-positive strains had more severe inflammation than those with non-intact cag PAI-ICEHptfs-negative, suggesting a possibility of a mutual correlation between these TFSS(s). Nature Publishing Group UK 2018-04-17 /pmc/articles/PMC5904169/ /pubmed/29666390 http://dx.doi.org/10.1038/s41598-018-24406-y Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Waskito, Langgeng Agung
Miftahussurur, Muhammad
Lusida, Maria Inge
Syam, Ari Fahrial
Suzuki, Rumiko
Subsomwong, Phawinee
Uchida, Tomohisa
Hamdan, Muhammad
Nasronudin
Yamaoka, Yoshio
Distribution and clinical associations of integrating conjugative elements and cag pathogenicity islands of Helicobacter pylori in Indonesia
title Distribution and clinical associations of integrating conjugative elements and cag pathogenicity islands of Helicobacter pylori in Indonesia
title_full Distribution and clinical associations of integrating conjugative elements and cag pathogenicity islands of Helicobacter pylori in Indonesia
title_fullStr Distribution and clinical associations of integrating conjugative elements and cag pathogenicity islands of Helicobacter pylori in Indonesia
title_full_unstemmed Distribution and clinical associations of integrating conjugative elements and cag pathogenicity islands of Helicobacter pylori in Indonesia
title_short Distribution and clinical associations of integrating conjugative elements and cag pathogenicity islands of Helicobacter pylori in Indonesia
title_sort distribution and clinical associations of integrating conjugative elements and cag pathogenicity islands of helicobacter pylori in indonesia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5904169/
https://www.ncbi.nlm.nih.gov/pubmed/29666390
http://dx.doi.org/10.1038/s41598-018-24406-y
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