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Surface pH changes suggest a role for H(+)/OH(−) channels in salinity response of Chara australis

To understand salt stress, the full impact of salinity on plant cell physiology has to be resolved. Electrical measurements suggest that salinity inhibits the proton pump and opens putative H(+)/OH(−) channels all over the cell surface of salt sensitive Chara australis (Beilby and Al Khazaaly 2009;...

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Autores principales: Absolonova, Marketa, Beilby, Mary J., Sommer, Aniela, Hoepflinger, Marion C., Foissner, Ilse
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5904247/
https://www.ncbi.nlm.nih.gov/pubmed/29247277
http://dx.doi.org/10.1007/s00709-017-1191-z
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author Absolonova, Marketa
Beilby, Mary J.
Sommer, Aniela
Hoepflinger, Marion C.
Foissner, Ilse
author_facet Absolonova, Marketa
Beilby, Mary J.
Sommer, Aniela
Hoepflinger, Marion C.
Foissner, Ilse
author_sort Absolonova, Marketa
collection PubMed
description To understand salt stress, the full impact of salinity on plant cell physiology has to be resolved. Electrical measurements suggest that salinity inhibits the proton pump and opens putative H(+)/OH(−) channels all over the cell surface of salt sensitive Chara australis (Beilby and Al Khazaaly 2009; Al Khazaaly and Beilby 2012). The channels open transiently at first, causing a characteristic noise in membrane potential difference (PD), and after longer exposure remain open with a typical current-voltage (I/V) profile, both abolished by the addition of 1 mM ZnCl(2), the main known blocker of animal H(+) channels. The cells were imaged with confocal microscopy, using fluorescein isothiocyanate (FITC) coupled to dextran 70 to illuminate the pH changes outside the cell wall in artificial fresh water (AFW) and in saline medium. In the early saline exposure, we observed alkaline patches (bright fluorescent spots) appearing transiently in random spatial distribution. After longer exposure, some of the spots became fixed in space. Saline also abolished or diminished the pH banding pattern observed in the untreated control cells. ZnCl(2) suppressed the alkaline spot formation in saline and the pH banding pattern in AFW. The osmotic component of the saline stress did not produce transient bright spots or affect banding. The displacement of H(+) from the cell wall charges, the H(+)/OH(−) channel conductance/density, and self-organization are discussed. No homologies to animal H(+) channels were found. Salinity activation of the H(+)/OH(−) channels might contribute to saline response in roots of land plants and leaves of aquatic angiosperms. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00709-017-1191-z) contains supplementary material, which is available to authorized users.
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spelling pubmed-59042472018-04-24 Surface pH changes suggest a role for H(+)/OH(−) channels in salinity response of Chara australis Absolonova, Marketa Beilby, Mary J. Sommer, Aniela Hoepflinger, Marion C. Foissner, Ilse Protoplasma Original Article To understand salt stress, the full impact of salinity on plant cell physiology has to be resolved. Electrical measurements suggest that salinity inhibits the proton pump and opens putative H(+)/OH(−) channels all over the cell surface of salt sensitive Chara australis (Beilby and Al Khazaaly 2009; Al Khazaaly and Beilby 2012). The channels open transiently at first, causing a characteristic noise in membrane potential difference (PD), and after longer exposure remain open with a typical current-voltage (I/V) profile, both abolished by the addition of 1 mM ZnCl(2), the main known blocker of animal H(+) channels. The cells were imaged with confocal microscopy, using fluorescein isothiocyanate (FITC) coupled to dextran 70 to illuminate the pH changes outside the cell wall in artificial fresh water (AFW) and in saline medium. In the early saline exposure, we observed alkaline patches (bright fluorescent spots) appearing transiently in random spatial distribution. After longer exposure, some of the spots became fixed in space. Saline also abolished or diminished the pH banding pattern observed in the untreated control cells. ZnCl(2) suppressed the alkaline spot formation in saline and the pH banding pattern in AFW. The osmotic component of the saline stress did not produce transient bright spots or affect banding. The displacement of H(+) from the cell wall charges, the H(+)/OH(−) channel conductance/density, and self-organization are discussed. No homologies to animal H(+) channels were found. Salinity activation of the H(+)/OH(−) channels might contribute to saline response in roots of land plants and leaves of aquatic angiosperms. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00709-017-1191-z) contains supplementary material, which is available to authorized users. Springer Vienna 2017-12-15 2018 /pmc/articles/PMC5904247/ /pubmed/29247277 http://dx.doi.org/10.1007/s00709-017-1191-z Text en © The Author(s) 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Absolonova, Marketa
Beilby, Mary J.
Sommer, Aniela
Hoepflinger, Marion C.
Foissner, Ilse
Surface pH changes suggest a role for H(+)/OH(−) channels in salinity response of Chara australis
title Surface pH changes suggest a role for H(+)/OH(−) channels in salinity response of Chara australis
title_full Surface pH changes suggest a role for H(+)/OH(−) channels in salinity response of Chara australis
title_fullStr Surface pH changes suggest a role for H(+)/OH(−) channels in salinity response of Chara australis
title_full_unstemmed Surface pH changes suggest a role for H(+)/OH(−) channels in salinity response of Chara australis
title_short Surface pH changes suggest a role for H(+)/OH(−) channels in salinity response of Chara australis
title_sort surface ph changes suggest a role for h(+)/oh(−) channels in salinity response of chara australis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5904247/
https://www.ncbi.nlm.nih.gov/pubmed/29247277
http://dx.doi.org/10.1007/s00709-017-1191-z
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