Cargando…

Prominent Levels of the Profibrotic Chemokine CCL18 during Peritonitis: In Vitro Downregulation by Vitamin D Receptor Agonists

Peritoneal dialysis (PD) is used as a renal replacement therapy, which can be limited by peritoneal membrane ultrafiltration failure (UFF) secondary to fibrotic processes. Peritonitis, a frequent complication of PD, is a major risk factor for peritoneal membrane fibrosis and UFF. Low peritoneal leve...

Descripción completa

Detalles Bibliográficos
Autores principales: Ossorio, Marta, Martínez, Virginia, Bajo, Maria-Auxiliadora, Del Peso, Gloria, Castro, Maria-José, Romero, Sara, Selgas, Rafael, Bellón, Teresa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5904802/
https://www.ncbi.nlm.nih.gov/pubmed/29850544
http://dx.doi.org/10.1155/2018/6415892
_version_ 1783315148155387904
author Ossorio, Marta
Martínez, Virginia
Bajo, Maria-Auxiliadora
Del Peso, Gloria
Castro, Maria-José
Romero, Sara
Selgas, Rafael
Bellón, Teresa
author_facet Ossorio, Marta
Martínez, Virginia
Bajo, Maria-Auxiliadora
Del Peso, Gloria
Castro, Maria-José
Romero, Sara
Selgas, Rafael
Bellón, Teresa
author_sort Ossorio, Marta
collection PubMed
description Peritoneal dialysis (PD) is used as a renal replacement therapy, which can be limited by peritoneal membrane ultrafiltration failure (UFF) secondary to fibrotic processes. Peritonitis, a frequent complication of PD, is a major risk factor for peritoneal membrane fibrosis and UFF. Low peritoneal levels of the chemokine CCL18 are associated with preservation of peritoneal membrane function in PD. Given that CCL18 is involved in fibrotic processes and recurrent peritonitis, it is a risk factor for peritoneal membrane failure; thus, we evaluated CCL18 concentrations in peritoneal effluents from patients undergoing peritonitis episodes. Pharmacological interventions aimed at diminishing the production of CCL18 were also explored. Fivefold higher CCL18 peritoneal concentrations were found during acute bacterial peritonitis, in parallel with the increased infiltration of macrophages. Unexpectedly, CCL18 was also highly (50-fold) increased during sterile eosinophilic peritonitis, and peritoneal eosinophils were found to express CCL18. In vitro treatment of peritoneal macrophages with the vitamin D receptor agonist paricalcitol was able to reduce the secretion and the expression of CCL18 in isolated peritoneal macrophages. In conclusion, our study suggests that the chemokine CCL18 can be a mediator of peritoneal membrane failure associated with peritonitis episodes as well as providing a new potential therapeutic target.
format Online
Article
Text
id pubmed-5904802
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-59048022018-05-30 Prominent Levels of the Profibrotic Chemokine CCL18 during Peritonitis: In Vitro Downregulation by Vitamin D Receptor Agonists Ossorio, Marta Martínez, Virginia Bajo, Maria-Auxiliadora Del Peso, Gloria Castro, Maria-José Romero, Sara Selgas, Rafael Bellón, Teresa Biomed Res Int Research Article Peritoneal dialysis (PD) is used as a renal replacement therapy, which can be limited by peritoneal membrane ultrafiltration failure (UFF) secondary to fibrotic processes. Peritonitis, a frequent complication of PD, is a major risk factor for peritoneal membrane fibrosis and UFF. Low peritoneal levels of the chemokine CCL18 are associated with preservation of peritoneal membrane function in PD. Given that CCL18 is involved in fibrotic processes and recurrent peritonitis, it is a risk factor for peritoneal membrane failure; thus, we evaluated CCL18 concentrations in peritoneal effluents from patients undergoing peritonitis episodes. Pharmacological interventions aimed at diminishing the production of CCL18 were also explored. Fivefold higher CCL18 peritoneal concentrations were found during acute bacterial peritonitis, in parallel with the increased infiltration of macrophages. Unexpectedly, CCL18 was also highly (50-fold) increased during sterile eosinophilic peritonitis, and peritoneal eosinophils were found to express CCL18. In vitro treatment of peritoneal macrophages with the vitamin D receptor agonist paricalcitol was able to reduce the secretion and the expression of CCL18 in isolated peritoneal macrophages. In conclusion, our study suggests that the chemokine CCL18 can be a mediator of peritoneal membrane failure associated with peritonitis episodes as well as providing a new potential therapeutic target. Hindawi 2018-04-04 /pmc/articles/PMC5904802/ /pubmed/29850544 http://dx.doi.org/10.1155/2018/6415892 Text en Copyright © 2018 Marta Ossorio et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ossorio, Marta
Martínez, Virginia
Bajo, Maria-Auxiliadora
Del Peso, Gloria
Castro, Maria-José
Romero, Sara
Selgas, Rafael
Bellón, Teresa
Prominent Levels of the Profibrotic Chemokine CCL18 during Peritonitis: In Vitro Downregulation by Vitamin D Receptor Agonists
title Prominent Levels of the Profibrotic Chemokine CCL18 during Peritonitis: In Vitro Downregulation by Vitamin D Receptor Agonists
title_full Prominent Levels of the Profibrotic Chemokine CCL18 during Peritonitis: In Vitro Downregulation by Vitamin D Receptor Agonists
title_fullStr Prominent Levels of the Profibrotic Chemokine CCL18 during Peritonitis: In Vitro Downregulation by Vitamin D Receptor Agonists
title_full_unstemmed Prominent Levels of the Profibrotic Chemokine CCL18 during Peritonitis: In Vitro Downregulation by Vitamin D Receptor Agonists
title_short Prominent Levels of the Profibrotic Chemokine CCL18 during Peritonitis: In Vitro Downregulation by Vitamin D Receptor Agonists
title_sort prominent levels of the profibrotic chemokine ccl18 during peritonitis: in vitro downregulation by vitamin d receptor agonists
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5904802/
https://www.ncbi.nlm.nih.gov/pubmed/29850544
http://dx.doi.org/10.1155/2018/6415892
work_keys_str_mv AT ossoriomarta prominentlevelsoftheprofibroticchemokineccl18duringperitonitisinvitrodownregulationbyvitamindreceptoragonists
AT martinezvirginia prominentlevelsoftheprofibroticchemokineccl18duringperitonitisinvitrodownregulationbyvitamindreceptoragonists
AT bajomariaauxiliadora prominentlevelsoftheprofibroticchemokineccl18duringperitonitisinvitrodownregulationbyvitamindreceptoragonists
AT delpesogloria prominentlevelsoftheprofibroticchemokineccl18duringperitonitisinvitrodownregulationbyvitamindreceptoragonists
AT castromariajose prominentlevelsoftheprofibroticchemokineccl18duringperitonitisinvitrodownregulationbyvitamindreceptoragonists
AT romerosara prominentlevelsoftheprofibroticchemokineccl18duringperitonitisinvitrodownregulationbyvitamindreceptoragonists
AT selgasrafael prominentlevelsoftheprofibroticchemokineccl18duringperitonitisinvitrodownregulationbyvitamindreceptoragonists
AT bellonteresa prominentlevelsoftheprofibroticchemokineccl18duringperitonitisinvitrodownregulationbyvitamindreceptoragonists