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Dual-color metal-induced and Förster resonance energy transfer for cell nanoscopy

We report a novel method, dual-color axial nanometric localization by metal-­induced energy transfer, and combine it with Förster resonance energy transfer (FRET) for resolving structural details in cells on the molecular level. We demonstrate the capability of this method on cytoskeletal elements a...

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Detalles Bibliográficos
Autores principales: Chizhik, Anna M., Wollnik, Carina, Ruhlandt, Daja, Karedla, Narain, Chizhik, Alexey I., Hauke, Lara, Hähnel, Dirk, Gregor, Ingo, Enderlein, Jörg, Rehfeldt, Florian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5905297/
https://www.ncbi.nlm.nih.gov/pubmed/29444956
http://dx.doi.org/10.1091/mbc.E17-05-0314
Descripción
Sumario:We report a novel method, dual-color axial nanometric localization by metal-­induced energy transfer, and combine it with Förster resonance energy transfer (FRET) for resolving structural details in cells on the molecular level. We demonstrate the capability of this method on cytoskeletal elements and adhesions in human mesenchymal stem cells. Our approach is based on fluorescence-lifetime-imaging microscopy and allows for precise determination of the three-dimensional architecture of stress fibers anchoring at focal adhesions, thus yielding crucial information to understand cell–matrix mechanics. In addition to resolving nanometric structural details along the z-axis, we use FRET to gain precise information on the distance between actin and vinculin at focal adhesions.