Cargando…
Dual-color metal-induced and Förster resonance energy transfer for cell nanoscopy
We report a novel method, dual-color axial nanometric localization by metal-induced energy transfer, and combine it with Förster resonance energy transfer (FRET) for resolving structural details in cells on the molecular level. We demonstrate the capability of this method on cytoskeletal elements a...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5905297/ https://www.ncbi.nlm.nih.gov/pubmed/29444956 http://dx.doi.org/10.1091/mbc.E17-05-0314 |
_version_ | 1783315243818024960 |
---|---|
author | Chizhik, Anna M. Wollnik, Carina Ruhlandt, Daja Karedla, Narain Chizhik, Alexey I. Hauke, Lara Hähnel, Dirk Gregor, Ingo Enderlein, Jörg Rehfeldt, Florian |
author_facet | Chizhik, Anna M. Wollnik, Carina Ruhlandt, Daja Karedla, Narain Chizhik, Alexey I. Hauke, Lara Hähnel, Dirk Gregor, Ingo Enderlein, Jörg Rehfeldt, Florian |
author_sort | Chizhik, Anna M. |
collection | PubMed |
description | We report a novel method, dual-color axial nanometric localization by metal-induced energy transfer, and combine it with Förster resonance energy transfer (FRET) for resolving structural details in cells on the molecular level. We demonstrate the capability of this method on cytoskeletal elements and adhesions in human mesenchymal stem cells. Our approach is based on fluorescence-lifetime-imaging microscopy and allows for precise determination of the three-dimensional architecture of stress fibers anchoring at focal adhesions, thus yielding crucial information to understand cell–matrix mechanics. In addition to resolving nanometric structural details along the z-axis, we use FRET to gain precise information on the distance between actin and vinculin at focal adhesions. |
format | Online Article Text |
id | pubmed-5905297 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-59052972018-06-16 Dual-color metal-induced and Förster resonance energy transfer for cell nanoscopy Chizhik, Anna M. Wollnik, Carina Ruhlandt, Daja Karedla, Narain Chizhik, Alexey I. Hauke, Lara Hähnel, Dirk Gregor, Ingo Enderlein, Jörg Rehfeldt, Florian Mol Biol Cell Articles We report a novel method, dual-color axial nanometric localization by metal-induced energy transfer, and combine it with Förster resonance energy transfer (FRET) for resolving structural details in cells on the molecular level. We demonstrate the capability of this method on cytoskeletal elements and adhesions in human mesenchymal stem cells. Our approach is based on fluorescence-lifetime-imaging microscopy and allows for precise determination of the three-dimensional architecture of stress fibers anchoring at focal adhesions, thus yielding crucial information to understand cell–matrix mechanics. In addition to resolving nanometric structural details along the z-axis, we use FRET to gain precise information on the distance between actin and vinculin at focal adhesions. The American Society for Cell Biology 2018-04-01 /pmc/articles/PMC5905297/ /pubmed/29444956 http://dx.doi.org/10.1091/mbc.E17-05-0314 Text en © 2018 Chizhik, Wollnik, et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0/ This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License. |
spellingShingle | Articles Chizhik, Anna M. Wollnik, Carina Ruhlandt, Daja Karedla, Narain Chizhik, Alexey I. Hauke, Lara Hähnel, Dirk Gregor, Ingo Enderlein, Jörg Rehfeldt, Florian Dual-color metal-induced and Förster resonance energy transfer for cell nanoscopy |
title | Dual-color metal-induced and Förster resonance energy transfer for cell nanoscopy |
title_full | Dual-color metal-induced and Förster resonance energy transfer for cell nanoscopy |
title_fullStr | Dual-color metal-induced and Förster resonance energy transfer for cell nanoscopy |
title_full_unstemmed | Dual-color metal-induced and Förster resonance energy transfer for cell nanoscopy |
title_short | Dual-color metal-induced and Förster resonance energy transfer for cell nanoscopy |
title_sort | dual-color metal-induced and förster resonance energy transfer for cell nanoscopy |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5905297/ https://www.ncbi.nlm.nih.gov/pubmed/29444956 http://dx.doi.org/10.1091/mbc.E17-05-0314 |
work_keys_str_mv | AT chizhikannam dualcolormetalinducedandforsterresonanceenergytransferforcellnanoscopy AT wollnikcarina dualcolormetalinducedandforsterresonanceenergytransferforcellnanoscopy AT ruhlandtdaja dualcolormetalinducedandforsterresonanceenergytransferforcellnanoscopy AT karedlanarain dualcolormetalinducedandforsterresonanceenergytransferforcellnanoscopy AT chizhikalexeyi dualcolormetalinducedandforsterresonanceenergytransferforcellnanoscopy AT haukelara dualcolormetalinducedandforsterresonanceenergytransferforcellnanoscopy AT hahneldirk dualcolormetalinducedandforsterresonanceenergytransferforcellnanoscopy AT gregoringo dualcolormetalinducedandforsterresonanceenergytransferforcellnanoscopy AT enderleinjorg dualcolormetalinducedandforsterresonanceenergytransferforcellnanoscopy AT rehfeldtflorian dualcolormetalinducedandforsterresonanceenergytransferforcellnanoscopy |