Cargando…

Dual-color metal-induced and Förster resonance energy transfer for cell nanoscopy

We report a novel method, dual-color axial nanometric localization by metal-­induced energy transfer, and combine it with Förster resonance energy transfer (FRET) for resolving structural details in cells on the molecular level. We demonstrate the capability of this method on cytoskeletal elements a...

Descripción completa

Detalles Bibliográficos
Autores principales: Chizhik, Anna M., Wollnik, Carina, Ruhlandt, Daja, Karedla, Narain, Chizhik, Alexey I., Hauke, Lara, Hähnel, Dirk, Gregor, Ingo, Enderlein, Jörg, Rehfeldt, Florian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5905297/
https://www.ncbi.nlm.nih.gov/pubmed/29444956
http://dx.doi.org/10.1091/mbc.E17-05-0314
_version_ 1783315243818024960
author Chizhik, Anna M.
Wollnik, Carina
Ruhlandt, Daja
Karedla, Narain
Chizhik, Alexey I.
Hauke, Lara
Hähnel, Dirk
Gregor, Ingo
Enderlein, Jörg
Rehfeldt, Florian
author_facet Chizhik, Anna M.
Wollnik, Carina
Ruhlandt, Daja
Karedla, Narain
Chizhik, Alexey I.
Hauke, Lara
Hähnel, Dirk
Gregor, Ingo
Enderlein, Jörg
Rehfeldt, Florian
author_sort Chizhik, Anna M.
collection PubMed
description We report a novel method, dual-color axial nanometric localization by metal-­induced energy transfer, and combine it with Förster resonance energy transfer (FRET) for resolving structural details in cells on the molecular level. We demonstrate the capability of this method on cytoskeletal elements and adhesions in human mesenchymal stem cells. Our approach is based on fluorescence-lifetime-imaging microscopy and allows for precise determination of the three-dimensional architecture of stress fibers anchoring at focal adhesions, thus yielding crucial information to understand cell–matrix mechanics. In addition to resolving nanometric structural details along the z-axis, we use FRET to gain precise information on the distance between actin and vinculin at focal adhesions.
format Online
Article
Text
id pubmed-5905297
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher The American Society for Cell Biology
record_format MEDLINE/PubMed
spelling pubmed-59052972018-06-16 Dual-color metal-induced and Förster resonance energy transfer for cell nanoscopy Chizhik, Anna M. Wollnik, Carina Ruhlandt, Daja Karedla, Narain Chizhik, Alexey I. Hauke, Lara Hähnel, Dirk Gregor, Ingo Enderlein, Jörg Rehfeldt, Florian Mol Biol Cell Articles We report a novel method, dual-color axial nanometric localization by metal-­induced energy transfer, and combine it with Förster resonance energy transfer (FRET) for resolving structural details in cells on the molecular level. We demonstrate the capability of this method on cytoskeletal elements and adhesions in human mesenchymal stem cells. Our approach is based on fluorescence-lifetime-imaging microscopy and allows for precise determination of the three-dimensional architecture of stress fibers anchoring at focal adhesions, thus yielding crucial information to understand cell–matrix mechanics. In addition to resolving nanometric structural details along the z-axis, we use FRET to gain precise information on the distance between actin and vinculin at focal adhesions. The American Society for Cell Biology 2018-04-01 /pmc/articles/PMC5905297/ /pubmed/29444956 http://dx.doi.org/10.1091/mbc.E17-05-0314 Text en © 2018 Chizhik, Wollnik, et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0/ This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
spellingShingle Articles
Chizhik, Anna M.
Wollnik, Carina
Ruhlandt, Daja
Karedla, Narain
Chizhik, Alexey I.
Hauke, Lara
Hähnel, Dirk
Gregor, Ingo
Enderlein, Jörg
Rehfeldt, Florian
Dual-color metal-induced and Förster resonance energy transfer for cell nanoscopy
title Dual-color metal-induced and Förster resonance energy transfer for cell nanoscopy
title_full Dual-color metal-induced and Förster resonance energy transfer for cell nanoscopy
title_fullStr Dual-color metal-induced and Förster resonance energy transfer for cell nanoscopy
title_full_unstemmed Dual-color metal-induced and Förster resonance energy transfer for cell nanoscopy
title_short Dual-color metal-induced and Förster resonance energy transfer for cell nanoscopy
title_sort dual-color metal-induced and förster resonance energy transfer for cell nanoscopy
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5905297/
https://www.ncbi.nlm.nih.gov/pubmed/29444956
http://dx.doi.org/10.1091/mbc.E17-05-0314
work_keys_str_mv AT chizhikannam dualcolormetalinducedandforsterresonanceenergytransferforcellnanoscopy
AT wollnikcarina dualcolormetalinducedandforsterresonanceenergytransferforcellnanoscopy
AT ruhlandtdaja dualcolormetalinducedandforsterresonanceenergytransferforcellnanoscopy
AT karedlanarain dualcolormetalinducedandforsterresonanceenergytransferforcellnanoscopy
AT chizhikalexeyi dualcolormetalinducedandforsterresonanceenergytransferforcellnanoscopy
AT haukelara dualcolormetalinducedandforsterresonanceenergytransferforcellnanoscopy
AT hahneldirk dualcolormetalinducedandforsterresonanceenergytransferforcellnanoscopy
AT gregoringo dualcolormetalinducedandforsterresonanceenergytransferforcellnanoscopy
AT enderleinjorg dualcolormetalinducedandforsterresonanceenergytransferforcellnanoscopy
AT rehfeldtflorian dualcolormetalinducedandforsterresonanceenergytransferforcellnanoscopy