Gene-based molecular analysis of COX1 in Echinococcus granulosus cysts isolated from naturally infected livestock in Riyadh, Saudi Arabia

The diversity and importance of Echinococcus species in domesticated animals in Saudi Arabia are poorly understood. In this study, 108 singular (hydatid) cysts were collected from goats (n = 25), sheep (n = 56) and camels (n = 27). DNA was extracted from the protoscoleces of individual fertile cysts...

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Autores principales: Metwally, Dina M., Qassim, Latifa E., Al-Turaiki, Isra M., Almeer, Rafa S., El-Khadragy, Manal F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5905957/
https://www.ncbi.nlm.nih.gov/pubmed/29668701
http://dx.doi.org/10.1371/journal.pone.0195016
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author Metwally, Dina M.
Qassim, Latifa E.
Al-Turaiki, Isra M.
Almeer, Rafa S.
El-Khadragy, Manal F.
author_facet Metwally, Dina M.
Qassim, Latifa E.
Al-Turaiki, Isra M.
Almeer, Rafa S.
El-Khadragy, Manal F.
author_sort Metwally, Dina M.
collection PubMed
description The diversity and importance of Echinococcus species in domesticated animals in Saudi Arabia are poorly understood. In this study, 108 singular (hydatid) cysts were collected from goats (n = 25), sheep (n = 56) and camels (n = 27). DNA was extracted from the protoscoleces of individual fertile cysts and used for polymerase chain reaction (PCR) amplification of mitochondrial subunit 1 of the cytochrome c oxidase 1 (cox1) gene. Amplicon sequencing results revealed the presence of Echinococcus granulosus sensustricto (s.s.) (genotypes G1–G3) in 16 of the17 sheep cysts and 2 of the 27 camel cysts.of these samples, 18 (2 camel and 16 sheep) were divided into genotypes G1, G2, and G3.
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spelling pubmed-59059572018-05-06 Gene-based molecular analysis of COX1 in Echinococcus granulosus cysts isolated from naturally infected livestock in Riyadh, Saudi Arabia Metwally, Dina M. Qassim, Latifa E. Al-Turaiki, Isra M. Almeer, Rafa S. El-Khadragy, Manal F. PLoS One Research Article The diversity and importance of Echinococcus species in domesticated animals in Saudi Arabia are poorly understood. In this study, 108 singular (hydatid) cysts were collected from goats (n = 25), sheep (n = 56) and camels (n = 27). DNA was extracted from the protoscoleces of individual fertile cysts and used for polymerase chain reaction (PCR) amplification of mitochondrial subunit 1 of the cytochrome c oxidase 1 (cox1) gene. Amplicon sequencing results revealed the presence of Echinococcus granulosus sensustricto (s.s.) (genotypes G1–G3) in 16 of the17 sheep cysts and 2 of the 27 camel cysts.of these samples, 18 (2 camel and 16 sheep) were divided into genotypes G1, G2, and G3. Public Library of Science 2018-04-18 /pmc/articles/PMC5905957/ /pubmed/29668701 http://dx.doi.org/10.1371/journal.pone.0195016 Text en © 2018 Metwally et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Metwally, Dina M.
Qassim, Latifa E.
Al-Turaiki, Isra M.
Almeer, Rafa S.
El-Khadragy, Manal F.
Gene-based molecular analysis of COX1 in Echinococcus granulosus cysts isolated from naturally infected livestock in Riyadh, Saudi Arabia
title Gene-based molecular analysis of COX1 in Echinococcus granulosus cysts isolated from naturally infected livestock in Riyadh, Saudi Arabia
title_full Gene-based molecular analysis of COX1 in Echinococcus granulosus cysts isolated from naturally infected livestock in Riyadh, Saudi Arabia
title_fullStr Gene-based molecular analysis of COX1 in Echinococcus granulosus cysts isolated from naturally infected livestock in Riyadh, Saudi Arabia
title_full_unstemmed Gene-based molecular analysis of COX1 in Echinococcus granulosus cysts isolated from naturally infected livestock in Riyadh, Saudi Arabia
title_short Gene-based molecular analysis of COX1 in Echinococcus granulosus cysts isolated from naturally infected livestock in Riyadh, Saudi Arabia
title_sort gene-based molecular analysis of cox1 in echinococcus granulosus cysts isolated from naturally infected livestock in riyadh, saudi arabia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5905957/
https://www.ncbi.nlm.nih.gov/pubmed/29668701
http://dx.doi.org/10.1371/journal.pone.0195016
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