Cargando…
Short Exposures to an Extremely Low-Frequency Magnetic Field (ELF MF) Enhance Protein but not mRNA Alkaline Phosphatase Expression in Human Osteosarcoma Cells
BACKGROUND: Among electromagnetic fields treatments used in orthopedics, extremely low-frequency magnetic fields (ELF MF) need more detailed information about the molecular mechanisms of their effects and exposure conditions. OBJECTIVE: Evaluation of the effects of an ELF MF exposure system, recentl...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Bentham Open
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5906974/ https://www.ncbi.nlm.nih.gov/pubmed/29760814 http://dx.doi.org/10.2174/1874091X01812010065 |
_version_ | 1783315451596505088 |
---|---|
author | Rescigno, Tania Capasso, Anna Bisceglia, Bruno Tecce, Mario Felice |
author_facet | Rescigno, Tania Capasso, Anna Bisceglia, Bruno Tecce, Mario Felice |
author_sort | Rescigno, Tania |
collection | PubMed |
description | BACKGROUND: Among electromagnetic fields treatments used in orthopedics, extremely low-frequency magnetic fields (ELF MF) need more detailed information about the molecular mechanisms of their effects and exposure conditions. OBJECTIVE: Evaluation of the effects of an ELF MF exposure system, recently introduced among current clinical treatments for fracture healing and other bone diseases, on Alkaline Phosphatase (ALP) activity and expression in a human osteosarcoma cell line (SaOS-2), as marker typically associated to osteogenesis and bone tissue regeneration. METHOD: Cells were exposed to the ELF MF physical stimulus (75 Hz, 1.5 mT) for 1h. Cell viability, enzymatic activity, protein and mRNA expression of alkaline phosphatase were then measured at different times after exposure (0, 4 and 24 h). RESULTS: Data demonstrate that this signal is active on an osteogenic process already one hour after exposure. Treatment was, in fact, capable, even after an exposure shorter than those commonly used in clinical applications, to significantly up-regulate alkaline phosphatase enzymatic activity. This regulation is produced essentially through an increase of ALP protein level, without changes of its mRNA concentration, while assessed magnetic field did not affect cell growth and viability and did not produce temperature variations. CONCLUSION: Tested low-frequency magnetic field affects cellular ALP expression with a posttranslational mechanism, without the involvement of regulations at gene transcription and mRNA level. This molecular effect is likely produced even within treated tissues during therapies with this signal and may be implicated in the induction of observed effects in treated patients. |
format | Online Article Text |
id | pubmed-5906974 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Bentham Open |
record_format | MEDLINE/PubMed |
spelling | pubmed-59069742018-05-14 Short Exposures to an Extremely Low-Frequency Magnetic Field (ELF MF) Enhance Protein but not mRNA Alkaline Phosphatase Expression in Human Osteosarcoma Cells Rescigno, Tania Capasso, Anna Bisceglia, Bruno Tecce, Mario Felice Open Biochem J Biochemistry BACKGROUND: Among electromagnetic fields treatments used in orthopedics, extremely low-frequency magnetic fields (ELF MF) need more detailed information about the molecular mechanisms of their effects and exposure conditions. OBJECTIVE: Evaluation of the effects of an ELF MF exposure system, recently introduced among current clinical treatments for fracture healing and other bone diseases, on Alkaline Phosphatase (ALP) activity and expression in a human osteosarcoma cell line (SaOS-2), as marker typically associated to osteogenesis and bone tissue regeneration. METHOD: Cells were exposed to the ELF MF physical stimulus (75 Hz, 1.5 mT) for 1h. Cell viability, enzymatic activity, protein and mRNA expression of alkaline phosphatase were then measured at different times after exposure (0, 4 and 24 h). RESULTS: Data demonstrate that this signal is active on an osteogenic process already one hour after exposure. Treatment was, in fact, capable, even after an exposure shorter than those commonly used in clinical applications, to significantly up-regulate alkaline phosphatase enzymatic activity. This regulation is produced essentially through an increase of ALP protein level, without changes of its mRNA concentration, while assessed magnetic field did not affect cell growth and viability and did not produce temperature variations. CONCLUSION: Tested low-frequency magnetic field affects cellular ALP expression with a posttranslational mechanism, without the involvement of regulations at gene transcription and mRNA level. This molecular effect is likely produced even within treated tissues during therapies with this signal and may be implicated in the induction of observed effects in treated patients. Bentham Open 2018-04-17 /pmc/articles/PMC5906974/ /pubmed/29760814 http://dx.doi.org/10.2174/1874091X01812010065 Text en © 2018 Rescigno et al. https://creativecommons.org/licenses/by/4.0/legalcode This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Biochemistry Rescigno, Tania Capasso, Anna Bisceglia, Bruno Tecce, Mario Felice Short Exposures to an Extremely Low-Frequency Magnetic Field (ELF MF) Enhance Protein but not mRNA Alkaline Phosphatase Expression in Human Osteosarcoma Cells |
title | Short Exposures to an Extremely Low-Frequency Magnetic Field (ELF MF) Enhance Protein but not mRNA Alkaline Phosphatase Expression in Human Osteosarcoma Cells |
title_full | Short Exposures to an Extremely Low-Frequency Magnetic Field (ELF MF) Enhance Protein but not mRNA Alkaline Phosphatase Expression in Human Osteosarcoma Cells |
title_fullStr | Short Exposures to an Extremely Low-Frequency Magnetic Field (ELF MF) Enhance Protein but not mRNA Alkaline Phosphatase Expression in Human Osteosarcoma Cells |
title_full_unstemmed | Short Exposures to an Extremely Low-Frequency Magnetic Field (ELF MF) Enhance Protein but not mRNA Alkaline Phosphatase Expression in Human Osteosarcoma Cells |
title_short | Short Exposures to an Extremely Low-Frequency Magnetic Field (ELF MF) Enhance Protein but not mRNA Alkaline Phosphatase Expression in Human Osteosarcoma Cells |
title_sort | short exposures to an extremely low-frequency magnetic field (elf mf) enhance protein but not mrna alkaline phosphatase expression in human osteosarcoma cells |
topic | Biochemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5906974/ https://www.ncbi.nlm.nih.gov/pubmed/29760814 http://dx.doi.org/10.2174/1874091X01812010065 |
work_keys_str_mv | AT rescignotania shortexposurestoanextremelylowfrequencymagneticfieldelfmfenhanceproteinbutnotmrnaalkalinephosphataseexpressioninhumanosteosarcomacells AT capassoanna shortexposurestoanextremelylowfrequencymagneticfieldelfmfenhanceproteinbutnotmrnaalkalinephosphataseexpressioninhumanosteosarcomacells AT biscegliabruno shortexposurestoanextremelylowfrequencymagneticfieldelfmfenhanceproteinbutnotmrnaalkalinephosphataseexpressioninhumanosteosarcomacells AT teccemariofelice shortexposurestoanextremelylowfrequencymagneticfieldelfmfenhanceproteinbutnotmrnaalkalinephosphataseexpressioninhumanosteosarcomacells |