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Wentong decoction cures allergic bronchial asthma by regulating the apoptosis imbalance of EOS

BACKGROUND: Eosinophils (EOS) is one of the most important cells involved in the pathogenesis of chronic airway inflammation in asthma, and its apoptosis is part of the mechanisms of asthma. Therefore, this study aimed to observe the effect of Chinese medicine Wentong decoction (WTD) in EOS apoptosi...

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Autores principales: Yan, Yue, Bao, Hai-Peng, Li, Chun-Lei, Shi, Qi, Kong, Yan-Hua, Yao, Ting, Li, You-Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5907368/
https://www.ncbi.nlm.nih.gov/pubmed/29713367
http://dx.doi.org/10.1186/s13020-018-0180-2
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author Yan, Yue
Bao, Hai-Peng
Li, Chun-Lei
Shi, Qi
Kong, Yan-Hua
Yao, Ting
Li, You-Lin
author_facet Yan, Yue
Bao, Hai-Peng
Li, Chun-Lei
Shi, Qi
Kong, Yan-Hua
Yao, Ting
Li, You-Lin
author_sort Yan, Yue
collection PubMed
description BACKGROUND: Eosinophils (EOS) is one of the most important cells involved in the pathogenesis of chronic airway inflammation in asthma, and its apoptosis is part of the mechanisms of asthma. Therefore, this study aimed to observe the effect of Chinese medicine Wentong decoction (WTD) in EOS apoptosis in asthmatic rats. This work also explored the mechanism of WTD regulation in EOS apoptosis and provided a new target for clinical treatment of asthma. METHODS: Asthmatic rats induced by ovalbumin were treated with WTD. Lung function of rats in each group was detected, and lung tissue pathology, EOS counts in blood and bronchoalveolar lavage fluid were observed. The degree of the EOS apoptosis in rats was detected. The expression content of interleukin (IL)-5, IL-10, chemokine (C–C motif) ligand 5 (CCL5), granulocyte–macrophage colony-stimulating factor (GM-CSF), transforming growth factor beta 1 (TGF-β1), interferon (IFN)-γ, and other cytokines in rat serum and the genes of Eotaxin mRNA, Fas mRNA, FasL mRNA, Fas/FasL and Bcl-2 mRNA in the lung tissues were determined. RESULTS: WTD can reduced airway resistance in rat models and improved airway compliance. The pathological changes of lung tissue in WTD group were significantly alleviated, at the same time, WTD could reduce the EOS count in the blood and BALF smears of the asthmatic model rats. Compared with the model group, the apoptosis degree of EOS significantly increased in rats in the WTD group. The expression of IL-5, CCL5, and GM-CSF in the serum and the expression of Eotaxin mRNA, Bcl-2 mRNA in the lung tissues in rats in the WTD group rats decreased. Moreover, the expression of IL-10, TGF-β1, and IFN-γ in the serum and the expression of Fas mRNA, FasL mRNA in the lung tissues in rats in the WTD group rats increased compared with that in rats in the model group. CONCLUSIONS: Wentong decoction may accelerate EOS apoptosis, reduce asthma inflammation, and alleviate the disease through regulating and controlling the factors related to the anti-apoptosis and pro-apoptosis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13020-018-0180-2) contains supplementary material, which is available to authorized users.
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spelling pubmed-59073682018-04-30 Wentong decoction cures allergic bronchial asthma by regulating the apoptosis imbalance of EOS Yan, Yue Bao, Hai-Peng Li, Chun-Lei Shi, Qi Kong, Yan-Hua Yao, Ting Li, You-Lin Chin Med Research BACKGROUND: Eosinophils (EOS) is one of the most important cells involved in the pathogenesis of chronic airway inflammation in asthma, and its apoptosis is part of the mechanisms of asthma. Therefore, this study aimed to observe the effect of Chinese medicine Wentong decoction (WTD) in EOS apoptosis in asthmatic rats. This work also explored the mechanism of WTD regulation in EOS apoptosis and provided a new target for clinical treatment of asthma. METHODS: Asthmatic rats induced by ovalbumin were treated with WTD. Lung function of rats in each group was detected, and lung tissue pathology, EOS counts in blood and bronchoalveolar lavage fluid were observed. The degree of the EOS apoptosis in rats was detected. The expression content of interleukin (IL)-5, IL-10, chemokine (C–C motif) ligand 5 (CCL5), granulocyte–macrophage colony-stimulating factor (GM-CSF), transforming growth factor beta 1 (TGF-β1), interferon (IFN)-γ, and other cytokines in rat serum and the genes of Eotaxin mRNA, Fas mRNA, FasL mRNA, Fas/FasL and Bcl-2 mRNA in the lung tissues were determined. RESULTS: WTD can reduced airway resistance in rat models and improved airway compliance. The pathological changes of lung tissue in WTD group were significantly alleviated, at the same time, WTD could reduce the EOS count in the blood and BALF smears of the asthmatic model rats. Compared with the model group, the apoptosis degree of EOS significantly increased in rats in the WTD group. The expression of IL-5, CCL5, and GM-CSF in the serum and the expression of Eotaxin mRNA, Bcl-2 mRNA in the lung tissues in rats in the WTD group rats decreased. Moreover, the expression of IL-10, TGF-β1, and IFN-γ in the serum and the expression of Fas mRNA, FasL mRNA in the lung tissues in rats in the WTD group rats increased compared with that in rats in the model group. CONCLUSIONS: Wentong decoction may accelerate EOS apoptosis, reduce asthma inflammation, and alleviate the disease through regulating and controlling the factors related to the anti-apoptosis and pro-apoptosis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13020-018-0180-2) contains supplementary material, which is available to authorized users. BioMed Central 2018-04-18 /pmc/articles/PMC5907368/ /pubmed/29713367 http://dx.doi.org/10.1186/s13020-018-0180-2 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Yan, Yue
Bao, Hai-Peng
Li, Chun-Lei
Shi, Qi
Kong, Yan-Hua
Yao, Ting
Li, You-Lin
Wentong decoction cures allergic bronchial asthma by regulating the apoptosis imbalance of EOS
title Wentong decoction cures allergic bronchial asthma by regulating the apoptosis imbalance of EOS
title_full Wentong decoction cures allergic bronchial asthma by regulating the apoptosis imbalance of EOS
title_fullStr Wentong decoction cures allergic bronchial asthma by regulating the apoptosis imbalance of EOS
title_full_unstemmed Wentong decoction cures allergic bronchial asthma by regulating the apoptosis imbalance of EOS
title_short Wentong decoction cures allergic bronchial asthma by regulating the apoptosis imbalance of EOS
title_sort wentong decoction cures allergic bronchial asthma by regulating the apoptosis imbalance of eos
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5907368/
https://www.ncbi.nlm.nih.gov/pubmed/29713367
http://dx.doi.org/10.1186/s13020-018-0180-2
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