Cargando…
High-mass-resolution MALDI mass spectrometry imaging reveals detailed spatial distribution of metabolites and lipids in roots of barley seedlings in response to salinity stress
INTRODUCTION: Mass spectrometry imaging (MSI) is a technology that enables the visualization of the spatial distribution of hundreds to thousands of metabolites in the same tissue section simultaneously. Roots are below-ground plant organs that anchor plants to the soil, take up water and nutrients,...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5907631/ https://www.ncbi.nlm.nih.gov/pubmed/29681790 http://dx.doi.org/10.1007/s11306-018-1359-3 |
_version_ | 1783315571659505664 |
---|---|
author | Sarabia, Lenin D. Boughton, Berin A. Rupasinghe, Thusitha van de Meene, Allison M. L. Callahan, Damien L. Hill, Camilla B. Roessner, Ute |
author_facet | Sarabia, Lenin D. Boughton, Berin A. Rupasinghe, Thusitha van de Meene, Allison M. L. Callahan, Damien L. Hill, Camilla B. Roessner, Ute |
author_sort | Sarabia, Lenin D. |
collection | PubMed |
description | INTRODUCTION: Mass spectrometry imaging (MSI) is a technology that enables the visualization of the spatial distribution of hundreds to thousands of metabolites in the same tissue section simultaneously. Roots are below-ground plant organs that anchor plants to the soil, take up water and nutrients, and sense and respond to external stresses. Physiological responses to salinity are multifaceted and have predominantly been studied using whole plant tissues that cannot resolve plant salinity responses spatially. OBJECTIVES: This study aimed to use a comprehensive approach to study the spatial distribution and profiles of metabolites, and to quantify the changes in the elemental content in young developing barley seminal roots before and after salinity stress. METHODS: Here, we used a combination of liquid chromatography–mass spectrometry (LC–MS), inductively coupled plasma mass spectrometry (ICP–MS), and matrix-assisted laser desorption/ionization (MALDI–MSI) platforms to profile and analyze the spatial distribution of ions, metabolites and lipids across three anatomically different barley root zones before and after a short-term salinity stress (150 mM NaCl). RESULTS: We localized, visualized and discriminated compounds in fine detail along longitudinal root sections and compared ion, metabolite, and lipid composition before and after salt stress. Large changes in the phosphatidylcholine (PC) profiles were observed as a response to salt stress with PC 34:n showing an overall reduction in salt treated roots. ICP–MS analysis quantified changes in the elemental content of roots with increases of Na(+) and decreases of K(+) content. CONCLUSION: Our results established the suitability of combining three mass spectrometry platforms to analyze and map ionic and metabolic responses to salinity stress in plant roots and to elucidate tolerance mechanisms in response to abiotic stress, such as salinity stress. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s11306-018-1359-3) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5907631 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-59076312018-04-20 High-mass-resolution MALDI mass spectrometry imaging reveals detailed spatial distribution of metabolites and lipids in roots of barley seedlings in response to salinity stress Sarabia, Lenin D. Boughton, Berin A. Rupasinghe, Thusitha van de Meene, Allison M. L. Callahan, Damien L. Hill, Camilla B. Roessner, Ute Metabolomics Original Article INTRODUCTION: Mass spectrometry imaging (MSI) is a technology that enables the visualization of the spatial distribution of hundreds to thousands of metabolites in the same tissue section simultaneously. Roots are below-ground plant organs that anchor plants to the soil, take up water and nutrients, and sense and respond to external stresses. Physiological responses to salinity are multifaceted and have predominantly been studied using whole plant tissues that cannot resolve plant salinity responses spatially. OBJECTIVES: This study aimed to use a comprehensive approach to study the spatial distribution and profiles of metabolites, and to quantify the changes in the elemental content in young developing barley seminal roots before and after salinity stress. METHODS: Here, we used a combination of liquid chromatography–mass spectrometry (LC–MS), inductively coupled plasma mass spectrometry (ICP–MS), and matrix-assisted laser desorption/ionization (MALDI–MSI) platforms to profile and analyze the spatial distribution of ions, metabolites and lipids across three anatomically different barley root zones before and after a short-term salinity stress (150 mM NaCl). RESULTS: We localized, visualized and discriminated compounds in fine detail along longitudinal root sections and compared ion, metabolite, and lipid composition before and after salt stress. Large changes in the phosphatidylcholine (PC) profiles were observed as a response to salt stress with PC 34:n showing an overall reduction in salt treated roots. ICP–MS analysis quantified changes in the elemental content of roots with increases of Na(+) and decreases of K(+) content. CONCLUSION: Our results established the suitability of combining three mass spectrometry platforms to analyze and map ionic and metabolic responses to salinity stress in plant roots and to elucidate tolerance mechanisms in response to abiotic stress, such as salinity stress. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s11306-018-1359-3) contains supplementary material, which is available to authorized users. Springer US 2018-04-19 2018 /pmc/articles/PMC5907631/ /pubmed/29681790 http://dx.doi.org/10.1007/s11306-018-1359-3 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Sarabia, Lenin D. Boughton, Berin A. Rupasinghe, Thusitha van de Meene, Allison M. L. Callahan, Damien L. Hill, Camilla B. Roessner, Ute High-mass-resolution MALDI mass spectrometry imaging reveals detailed spatial distribution of metabolites and lipids in roots of barley seedlings in response to salinity stress |
title | High-mass-resolution MALDI mass spectrometry imaging reveals detailed spatial distribution of metabolites and lipids in roots of barley seedlings in response to salinity stress |
title_full | High-mass-resolution MALDI mass spectrometry imaging reveals detailed spatial distribution of metabolites and lipids in roots of barley seedlings in response to salinity stress |
title_fullStr | High-mass-resolution MALDI mass spectrometry imaging reveals detailed spatial distribution of metabolites and lipids in roots of barley seedlings in response to salinity stress |
title_full_unstemmed | High-mass-resolution MALDI mass spectrometry imaging reveals detailed spatial distribution of metabolites and lipids in roots of barley seedlings in response to salinity stress |
title_short | High-mass-resolution MALDI mass spectrometry imaging reveals detailed spatial distribution of metabolites and lipids in roots of barley seedlings in response to salinity stress |
title_sort | high-mass-resolution maldi mass spectrometry imaging reveals detailed spatial distribution of metabolites and lipids in roots of barley seedlings in response to salinity stress |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5907631/ https://www.ncbi.nlm.nih.gov/pubmed/29681790 http://dx.doi.org/10.1007/s11306-018-1359-3 |
work_keys_str_mv | AT sarabialenind highmassresolutionmaldimassspectrometryimagingrevealsdetailedspatialdistributionofmetabolitesandlipidsinrootsofbarleyseedlingsinresponsetosalinitystress AT boughtonberina highmassresolutionmaldimassspectrometryimagingrevealsdetailedspatialdistributionofmetabolitesandlipidsinrootsofbarleyseedlingsinresponsetosalinitystress AT rupasinghethusitha highmassresolutionmaldimassspectrometryimagingrevealsdetailedspatialdistributionofmetabolitesandlipidsinrootsofbarleyseedlingsinresponsetosalinitystress AT vandemeeneallisonml highmassresolutionmaldimassspectrometryimagingrevealsdetailedspatialdistributionofmetabolitesandlipidsinrootsofbarleyseedlingsinresponsetosalinitystress AT callahandamienl highmassresolutionmaldimassspectrometryimagingrevealsdetailedspatialdistributionofmetabolitesandlipidsinrootsofbarleyseedlingsinresponsetosalinitystress AT hillcamillab highmassresolutionmaldimassspectrometryimagingrevealsdetailedspatialdistributionofmetabolitesandlipidsinrootsofbarleyseedlingsinresponsetosalinitystress AT roessnerute highmassresolutionmaldimassspectrometryimagingrevealsdetailedspatialdistributionofmetabolitesandlipidsinrootsofbarleyseedlingsinresponsetosalinitystress |