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Estrogen Inhibits the Growth of MCF‐7 Cell Variants Resistant to Transforming Growth Factor‐beta

Human breast cancer MCF‐7 cells containing estrogen receptor are killed by transforming growth factor‐beta (TGF‐β). We isolated variants of MCF‐7 highly resistant to TGF‐β. Variants ES‐1 and ES‐4 were cloned, and the growth of ES‐1 and ES‐4 was found to be inhibited by estradiol, whereas estradiol s...

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Detalles Bibliográficos
Autores principales: Hagino, Yoshiaki, Mawatari, Masasumi, Yoshimura, Akihiko, Kohno, Kimitoshi, Kobayashi, Michio, Kuwano, Michihiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1988
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5907766/
https://www.ncbi.nlm.nih.gov/pubmed/3128508
http://dx.doi.org/10.1111/j.1349-7006.1988.tb00013.x
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author Hagino, Yoshiaki
Mawatari, Masasumi
Yoshimura, Akihiko
Kohno, Kimitoshi
Kobayashi, Michio
Kuwano, Michihiko
author_facet Hagino, Yoshiaki
Mawatari, Masasumi
Yoshimura, Akihiko
Kohno, Kimitoshi
Kobayashi, Michio
Kuwano, Michihiko
author_sort Hagino, Yoshiaki
collection PubMed
description Human breast cancer MCF‐7 cells containing estrogen receptor are killed by transforming growth factor‐beta (TGF‐β). We isolated variants of MCF‐7 highly resistant to TGF‐β. Variants ES‐1 and ES‐4 were cloned, and the growth of ES‐1 and ES‐4 was found to be inhibited by estradiol, whereas estradiol stimulated the growth of the parental MCF‐7 cells. ES‐1 cells contained about 2‐fold higher level of estradiol receptor than MCF‐7 cells. Addition of estradiol to the culture medium for MCF‐7 and the variant changed the expression of several secreted proteins. The repertoire of secreted proteins was markedly altered in the variant. Polypeptides of molecular weight 52,000 (52 K), 65 K and 160 K were increased about 10‐ to 50‐fold in both estradiol‐treated MCF‐7 and ES‐1 cells. Polypeptide of 130 K was decreased in estradiol‐treated ES‐1 cells while this polypeptide was increased about 4‐fold in estradiol‐treated MCF‐7, as compared with untreated MCF‐7. Polypeptide of 100 K was specifically secreted in ES‐1 whether or not estradiol was present, but there appeared to be no significant amount of the 100 K protein in MCF‐7. The estradiol‐hypersensitive phenotype is discussed in relation to its aberrant expression of secreting proteins.
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spelling pubmed-59077662018-05-11 Estrogen Inhibits the Growth of MCF‐7 Cell Variants Resistant to Transforming Growth Factor‐beta Hagino, Yoshiaki Mawatari, Masasumi Yoshimura, Akihiko Kohno, Kimitoshi Kobayashi, Michio Kuwano, Michihiko Jpn J Cancer Res Article Human breast cancer MCF‐7 cells containing estrogen receptor are killed by transforming growth factor‐beta (TGF‐β). We isolated variants of MCF‐7 highly resistant to TGF‐β. Variants ES‐1 and ES‐4 were cloned, and the growth of ES‐1 and ES‐4 was found to be inhibited by estradiol, whereas estradiol stimulated the growth of the parental MCF‐7 cells. ES‐1 cells contained about 2‐fold higher level of estradiol receptor than MCF‐7 cells. Addition of estradiol to the culture medium for MCF‐7 and the variant changed the expression of several secreted proteins. The repertoire of secreted proteins was markedly altered in the variant. Polypeptides of molecular weight 52,000 (52 K), 65 K and 160 K were increased about 10‐ to 50‐fold in both estradiol‐treated MCF‐7 and ES‐1 cells. Polypeptide of 130 K was decreased in estradiol‐treated ES‐1 cells while this polypeptide was increased about 4‐fold in estradiol‐treated MCF‐7, as compared with untreated MCF‐7. Polypeptide of 100 K was specifically secreted in ES‐1 whether or not estradiol was present, but there appeared to be no significant amount of the 100 K protein in MCF‐7. The estradiol‐hypersensitive phenotype is discussed in relation to its aberrant expression of secreting proteins. Blackwell Publishing Ltd 1988-01 /pmc/articles/PMC5907766/ /pubmed/3128508 http://dx.doi.org/10.1111/j.1349-7006.1988.tb00013.x Text en
spellingShingle Article
Hagino, Yoshiaki
Mawatari, Masasumi
Yoshimura, Akihiko
Kohno, Kimitoshi
Kobayashi, Michio
Kuwano, Michihiko
Estrogen Inhibits the Growth of MCF‐7 Cell Variants Resistant to Transforming Growth Factor‐beta
title Estrogen Inhibits the Growth of MCF‐7 Cell Variants Resistant to Transforming Growth Factor‐beta
title_full Estrogen Inhibits the Growth of MCF‐7 Cell Variants Resistant to Transforming Growth Factor‐beta
title_fullStr Estrogen Inhibits the Growth of MCF‐7 Cell Variants Resistant to Transforming Growth Factor‐beta
title_full_unstemmed Estrogen Inhibits the Growth of MCF‐7 Cell Variants Resistant to Transforming Growth Factor‐beta
title_short Estrogen Inhibits the Growth of MCF‐7 Cell Variants Resistant to Transforming Growth Factor‐beta
title_sort estrogen inhibits the growth of mcf‐7 cell variants resistant to transforming growth factor‐beta
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5907766/
https://www.ncbi.nlm.nih.gov/pubmed/3128508
http://dx.doi.org/10.1111/j.1349-7006.1988.tb00013.x
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