DEFECT IN THE DEVELOPMENT OF THERMOTOLERANCE IN THE MOUSE TEMPERATURE‐SENSITIVE MUTANT ts85 LACKING UBIQUITIN‐ACTIVATING ENZYME

We investigated the effect of heat shock on the development of thermotolerance using mouse FM3A cells and the temperature‐sensitive mutant ts85. The shift‐up incubation of FM3A from 33 to 39.5° induced thermotolerance to subsequent heating at 44°. In contrast, the similar treatment of ts85 at the no...

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Detalles Bibliográficos
Autores principales: Mizuno, Satoshi, Ohkawara, Akiko, Suzuki, Kazuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1988
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5907768/
https://www.ncbi.nlm.nih.gov/pubmed/2833480
http://dx.doi.org/10.1111/j.1349-7006.1988.tb00005.x
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author Mizuno, Satoshi
Ohkawara, Akiko
Suzuki, Kazuo
author_facet Mizuno, Satoshi
Ohkawara, Akiko
Suzuki, Kazuo
author_sort Mizuno, Satoshi
collection PubMed
description We investigated the effect of heat shock on the development of thermotolerance using mouse FM3A cells and the temperature‐sensitive mutant ts85. The shift‐up incubation of FM3A from 33 to 39.5° induced thermotolerance to subsequent heating at 44°. In contrast, the similar treatment of ts85 at the nonpermissive temperature of 39.5° could not induce thermotolerance. Furthermore, when ts85 cells were treated at 33° after being heated at 44°, they developed a reduced level of thermotolerance as compared with that developed in FM3A cells. Since ts85 cells are defective in ubiquitin‐activating enzyme, these results suggest a role of the ubiquitin‐protein conjugation system in the development of thermotolerance.
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spelling pubmed-59077682018-05-11 DEFECT IN THE DEVELOPMENT OF THERMOTOLERANCE IN THE MOUSE TEMPERATURE‐SENSITIVE MUTANT ts85 LACKING UBIQUITIN‐ACTIVATING ENZYME Mizuno, Satoshi Ohkawara, Akiko Suzuki, Kazuo Jpn J Cancer Res Rapid Communication We investigated the effect of heat shock on the development of thermotolerance using mouse FM3A cells and the temperature‐sensitive mutant ts85. The shift‐up incubation of FM3A from 33 to 39.5° induced thermotolerance to subsequent heating at 44°. In contrast, the similar treatment of ts85 at the nonpermissive temperature of 39.5° could not induce thermotolerance. Furthermore, when ts85 cells were treated at 33° after being heated at 44°, they developed a reduced level of thermotolerance as compared with that developed in FM3A cells. Since ts85 cells are defective in ubiquitin‐activating enzyme, these results suggest a role of the ubiquitin‐protein conjugation system in the development of thermotolerance. Blackwell Publishing Ltd 1988-01 /pmc/articles/PMC5907768/ /pubmed/2833480 http://dx.doi.org/10.1111/j.1349-7006.1988.tb00005.x Text en
spellingShingle Rapid Communication
Mizuno, Satoshi
Ohkawara, Akiko
Suzuki, Kazuo
DEFECT IN THE DEVELOPMENT OF THERMOTOLERANCE IN THE MOUSE TEMPERATURE‐SENSITIVE MUTANT ts85 LACKING UBIQUITIN‐ACTIVATING ENZYME
title DEFECT IN THE DEVELOPMENT OF THERMOTOLERANCE IN THE MOUSE TEMPERATURE‐SENSITIVE MUTANT ts85 LACKING UBIQUITIN‐ACTIVATING ENZYME
title_full DEFECT IN THE DEVELOPMENT OF THERMOTOLERANCE IN THE MOUSE TEMPERATURE‐SENSITIVE MUTANT ts85 LACKING UBIQUITIN‐ACTIVATING ENZYME
title_fullStr DEFECT IN THE DEVELOPMENT OF THERMOTOLERANCE IN THE MOUSE TEMPERATURE‐SENSITIVE MUTANT ts85 LACKING UBIQUITIN‐ACTIVATING ENZYME
title_full_unstemmed DEFECT IN THE DEVELOPMENT OF THERMOTOLERANCE IN THE MOUSE TEMPERATURE‐SENSITIVE MUTANT ts85 LACKING UBIQUITIN‐ACTIVATING ENZYME
title_short DEFECT IN THE DEVELOPMENT OF THERMOTOLERANCE IN THE MOUSE TEMPERATURE‐SENSITIVE MUTANT ts85 LACKING UBIQUITIN‐ACTIVATING ENZYME
title_sort defect in the development of thermotolerance in the mouse temperature‐sensitive mutant ts85 lacking ubiquitin‐activating enzyme
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5907768/
https://www.ncbi.nlm.nih.gov/pubmed/2833480
http://dx.doi.org/10.1111/j.1349-7006.1988.tb00005.x
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AT ohkawaraakiko defectinthedevelopmentofthermotoleranceinthemousetemperaturesensitivemutantts85lackingubiquitinactivatingenzyme
AT suzukikazuo defectinthedevelopmentofthermotoleranceinthemousetemperaturesensitivemutantts85lackingubiquitinactivatingenzyme

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