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Replication study: androgen receptor splice variants determine taxane sensitivity in prostate cancer

In 2015, as part of the Prostate Cancer Foundation–Movember Foundation Reproducibility Initiative, we published a Registered Report (Shan et al., 2015) that described how we intended to replicate selected experiments from the paper “Androgen Receptor Splice Variants Determine Taxane Sensitivity in P...

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Autores principales: Shan, Xiaochuan, Danet-Desnoyers, Gwenn, Aird, Fraser, Kandela, Irawati, Tsui, Rachel, Perfito, Nicole, Iorns, Elizabeth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5907780/
https://www.ncbi.nlm.nih.gov/pubmed/29682426
http://dx.doi.org/10.7717/peerj.4661
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author Shan, Xiaochuan
Danet-Desnoyers, Gwenn
Aird, Fraser
Kandela, Irawati
Tsui, Rachel
Perfito, Nicole
Iorns, Elizabeth
author_facet Shan, Xiaochuan
Danet-Desnoyers, Gwenn
Aird, Fraser
Kandela, Irawati
Tsui, Rachel
Perfito, Nicole
Iorns, Elizabeth
author_sort Shan, Xiaochuan
collection PubMed
description In 2015, as part of the Prostate Cancer Foundation–Movember Foundation Reproducibility Initiative, we published a Registered Report (Shan et al., 2015) that described how we intended to replicate selected experiments from the paper “Androgen Receptor Splice Variants Determine Taxane Sensitivity in Prostate Cancer” (Thadani-Mulero et al., 2014). Here we report the results of those experiments. Growth of tumor xenografts from two prostate cancer xenograft lines, LuCaP 86.2, which expresses wild-type androgen receptor (AR) and AR variant 567, and LuCaP 23.1, which expresses wild-type AR and AR variant 7, were not affected by docetaxel treatment. The LuCaP 23.1 tumor xenografts grew slower than in the original study. This result is different from the original study, which reported significant reduction of tumor growth in the LuCaP 86.2. Furthermore, we were unable to detect ARv7 in the LuCaP 23.1, although we used the antibody as stated in the original study and ensured that it was detecting ARv7 via a known positive control (22rv1, Hörnberg et al., 2011). Finally, we report a meta-analysis of the result.
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spelling pubmed-59077802018-04-22 Replication study: androgen receptor splice variants determine taxane sensitivity in prostate cancer Shan, Xiaochuan Danet-Desnoyers, Gwenn Aird, Fraser Kandela, Irawati Tsui, Rachel Perfito, Nicole Iorns, Elizabeth PeerJ Cell Biology In 2015, as part of the Prostate Cancer Foundation–Movember Foundation Reproducibility Initiative, we published a Registered Report (Shan et al., 2015) that described how we intended to replicate selected experiments from the paper “Androgen Receptor Splice Variants Determine Taxane Sensitivity in Prostate Cancer” (Thadani-Mulero et al., 2014). Here we report the results of those experiments. Growth of tumor xenografts from two prostate cancer xenograft lines, LuCaP 86.2, which expresses wild-type androgen receptor (AR) and AR variant 567, and LuCaP 23.1, which expresses wild-type AR and AR variant 7, were not affected by docetaxel treatment. The LuCaP 23.1 tumor xenografts grew slower than in the original study. This result is different from the original study, which reported significant reduction of tumor growth in the LuCaP 86.2. Furthermore, we were unable to detect ARv7 in the LuCaP 23.1, although we used the antibody as stated in the original study and ensured that it was detecting ARv7 via a known positive control (22rv1, Hörnberg et al., 2011). Finally, we report a meta-analysis of the result. PeerJ Inc. 2018-04-16 /pmc/articles/PMC5907780/ /pubmed/29682426 http://dx.doi.org/10.7717/peerj.4661 Text en © 2018 Shan et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Cell Biology
Shan, Xiaochuan
Danet-Desnoyers, Gwenn
Aird, Fraser
Kandela, Irawati
Tsui, Rachel
Perfito, Nicole
Iorns, Elizabeth
Replication study: androgen receptor splice variants determine taxane sensitivity in prostate cancer
title Replication study: androgen receptor splice variants determine taxane sensitivity in prostate cancer
title_full Replication study: androgen receptor splice variants determine taxane sensitivity in prostate cancer
title_fullStr Replication study: androgen receptor splice variants determine taxane sensitivity in prostate cancer
title_full_unstemmed Replication study: androgen receptor splice variants determine taxane sensitivity in prostate cancer
title_short Replication study: androgen receptor splice variants determine taxane sensitivity in prostate cancer
title_sort replication study: androgen receptor splice variants determine taxane sensitivity in prostate cancer
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5907780/
https://www.ncbi.nlm.nih.gov/pubmed/29682426
http://dx.doi.org/10.7717/peerj.4661
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