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Replication study: androgen receptor splice variants determine taxane sensitivity in prostate cancer
In 2015, as part of the Prostate Cancer Foundation–Movember Foundation Reproducibility Initiative, we published a Registered Report (Shan et al., 2015) that described how we intended to replicate selected experiments from the paper “Androgen Receptor Splice Variants Determine Taxane Sensitivity in P...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5907780/ https://www.ncbi.nlm.nih.gov/pubmed/29682426 http://dx.doi.org/10.7717/peerj.4661 |
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author | Shan, Xiaochuan Danet-Desnoyers, Gwenn Aird, Fraser Kandela, Irawati Tsui, Rachel Perfito, Nicole Iorns, Elizabeth |
author_facet | Shan, Xiaochuan Danet-Desnoyers, Gwenn Aird, Fraser Kandela, Irawati Tsui, Rachel Perfito, Nicole Iorns, Elizabeth |
author_sort | Shan, Xiaochuan |
collection | PubMed |
description | In 2015, as part of the Prostate Cancer Foundation–Movember Foundation Reproducibility Initiative, we published a Registered Report (Shan et al., 2015) that described how we intended to replicate selected experiments from the paper “Androgen Receptor Splice Variants Determine Taxane Sensitivity in Prostate Cancer” (Thadani-Mulero et al., 2014). Here we report the results of those experiments. Growth of tumor xenografts from two prostate cancer xenograft lines, LuCaP 86.2, which expresses wild-type androgen receptor (AR) and AR variant 567, and LuCaP 23.1, which expresses wild-type AR and AR variant 7, were not affected by docetaxel treatment. The LuCaP 23.1 tumor xenografts grew slower than in the original study. This result is different from the original study, which reported significant reduction of tumor growth in the LuCaP 86.2. Furthermore, we were unable to detect ARv7 in the LuCaP 23.1, although we used the antibody as stated in the original study and ensured that it was detecting ARv7 via a known positive control (22rv1, Hörnberg et al., 2011). Finally, we report a meta-analysis of the result. |
format | Online Article Text |
id | pubmed-5907780 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-59077802018-04-22 Replication study: androgen receptor splice variants determine taxane sensitivity in prostate cancer Shan, Xiaochuan Danet-Desnoyers, Gwenn Aird, Fraser Kandela, Irawati Tsui, Rachel Perfito, Nicole Iorns, Elizabeth PeerJ Cell Biology In 2015, as part of the Prostate Cancer Foundation–Movember Foundation Reproducibility Initiative, we published a Registered Report (Shan et al., 2015) that described how we intended to replicate selected experiments from the paper “Androgen Receptor Splice Variants Determine Taxane Sensitivity in Prostate Cancer” (Thadani-Mulero et al., 2014). Here we report the results of those experiments. Growth of tumor xenografts from two prostate cancer xenograft lines, LuCaP 86.2, which expresses wild-type androgen receptor (AR) and AR variant 567, and LuCaP 23.1, which expresses wild-type AR and AR variant 7, were not affected by docetaxel treatment. The LuCaP 23.1 tumor xenografts grew slower than in the original study. This result is different from the original study, which reported significant reduction of tumor growth in the LuCaP 86.2. Furthermore, we were unable to detect ARv7 in the LuCaP 23.1, although we used the antibody as stated in the original study and ensured that it was detecting ARv7 via a known positive control (22rv1, Hörnberg et al., 2011). Finally, we report a meta-analysis of the result. PeerJ Inc. 2018-04-16 /pmc/articles/PMC5907780/ /pubmed/29682426 http://dx.doi.org/10.7717/peerj.4661 Text en © 2018 Shan et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Cell Biology Shan, Xiaochuan Danet-Desnoyers, Gwenn Aird, Fraser Kandela, Irawati Tsui, Rachel Perfito, Nicole Iorns, Elizabeth Replication study: androgen receptor splice variants determine taxane sensitivity in prostate cancer |
title | Replication study: androgen receptor splice variants determine taxane sensitivity in prostate cancer |
title_full | Replication study: androgen receptor splice variants determine taxane sensitivity in prostate cancer |
title_fullStr | Replication study: androgen receptor splice variants determine taxane sensitivity in prostate cancer |
title_full_unstemmed | Replication study: androgen receptor splice variants determine taxane sensitivity in prostate cancer |
title_short | Replication study: androgen receptor splice variants determine taxane sensitivity in prostate cancer |
title_sort | replication study: androgen receptor splice variants determine taxane sensitivity in prostate cancer |
topic | Cell Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5907780/ https://www.ncbi.nlm.nih.gov/pubmed/29682426 http://dx.doi.org/10.7717/peerj.4661 |
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