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Electrical field stimulation-induced contractions on Pantherophis guttatus corpora cavernosa and aortae

A tetrodotoxin (TTX)-resistant mechanism is responsible for the electrical field stimulation (EFS)-induced contractions and relaxations of Crotalus durissus terrificus corpora cavernosa. Here it was investigated whether this mechanism also occurs in corpora cavernosa and aortae of the non-venomous s...

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Autores principales: Campos, Rafael, Mónica, Fabíola Z., Justo, Alberto Fernando Oliveira, Cogo, José Carlos, Oliveira, Edvana de Toledo, Moreno, Ronilson Agnaldo, Antunes, Edson, De Nucci, Gilberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5908133/
https://www.ncbi.nlm.nih.gov/pubmed/29672643
http://dx.doi.org/10.1371/journal.pone.0196123
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author Campos, Rafael
Mónica, Fabíola Z.
Justo, Alberto Fernando Oliveira
Cogo, José Carlos
Oliveira, Edvana de Toledo
Moreno, Ronilson Agnaldo
Antunes, Edson
De Nucci, Gilberto
author_facet Campos, Rafael
Mónica, Fabíola Z.
Justo, Alberto Fernando Oliveira
Cogo, José Carlos
Oliveira, Edvana de Toledo
Moreno, Ronilson Agnaldo
Antunes, Edson
De Nucci, Gilberto
author_sort Campos, Rafael
collection PubMed
description A tetrodotoxin (TTX)-resistant mechanism is responsible for the electrical field stimulation (EFS)-induced contractions and relaxations of Crotalus durissus terrificus corpora cavernosa. Here it was investigated whether this mechanism also occurs in corpora cavernosa and aortae of the non-venomous snake Pantherophis guttatus corpora cavernosa and aortae. Corpora cavernosa and aortic rings isolated from Pantherophis guttatus snake were mounted in organ bath system for isometric tension recording. EFS-induced contractions in both tissues were performed in the presence and absence of guanethidine (30 μM), phentolamine (10 μM) and tetrodotoxin (1 μM). In another set of experiments, the endothelium was removed from aortic rings and EFS-induced contractions were performed in the denuded rings. Electrical field stimulation-induced contractions were frequency-dependent in Pantherophis guttatus corpora cavernosa and aortic rings. The contractions were significantly reduced in the presence of guanethidine (30 μM) or phentolamine (10 μM). Pre-treatment with tetrodotoxin had no effect on the EFS-induced contractions of either corpora cavernosa or aortic rings. Surprisingly, the EFS-induced contractions of aortic rings denuded of endothelium were almost abolished. These results indicate that the TTX-resistant mechanism is present in EFS-induced contractions of Pantherophis guttatus corpora cavernosa and aortae. The experiments performed in the aorta indicate that the endothelium is the main source for the release of catecholamines induced by EFS.
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spelling pubmed-59081332018-05-04 Electrical field stimulation-induced contractions on Pantherophis guttatus corpora cavernosa and aortae Campos, Rafael Mónica, Fabíola Z. Justo, Alberto Fernando Oliveira Cogo, José Carlos Oliveira, Edvana de Toledo Moreno, Ronilson Agnaldo Antunes, Edson De Nucci, Gilberto PLoS One Research Article A tetrodotoxin (TTX)-resistant mechanism is responsible for the electrical field stimulation (EFS)-induced contractions and relaxations of Crotalus durissus terrificus corpora cavernosa. Here it was investigated whether this mechanism also occurs in corpora cavernosa and aortae of the non-venomous snake Pantherophis guttatus corpora cavernosa and aortae. Corpora cavernosa and aortic rings isolated from Pantherophis guttatus snake were mounted in organ bath system for isometric tension recording. EFS-induced contractions in both tissues were performed in the presence and absence of guanethidine (30 μM), phentolamine (10 μM) and tetrodotoxin (1 μM). In another set of experiments, the endothelium was removed from aortic rings and EFS-induced contractions were performed in the denuded rings. Electrical field stimulation-induced contractions were frequency-dependent in Pantherophis guttatus corpora cavernosa and aortic rings. The contractions were significantly reduced in the presence of guanethidine (30 μM) or phentolamine (10 μM). Pre-treatment with tetrodotoxin had no effect on the EFS-induced contractions of either corpora cavernosa or aortic rings. Surprisingly, the EFS-induced contractions of aortic rings denuded of endothelium were almost abolished. These results indicate that the TTX-resistant mechanism is present in EFS-induced contractions of Pantherophis guttatus corpora cavernosa and aortae. The experiments performed in the aorta indicate that the endothelium is the main source for the release of catecholamines induced by EFS. Public Library of Science 2018-04-19 /pmc/articles/PMC5908133/ /pubmed/29672643 http://dx.doi.org/10.1371/journal.pone.0196123 Text en © 2018 Campos et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Campos, Rafael
Mónica, Fabíola Z.
Justo, Alberto Fernando Oliveira
Cogo, José Carlos
Oliveira, Edvana de Toledo
Moreno, Ronilson Agnaldo
Antunes, Edson
De Nucci, Gilberto
Electrical field stimulation-induced contractions on Pantherophis guttatus corpora cavernosa and aortae
title Electrical field stimulation-induced contractions on Pantherophis guttatus corpora cavernosa and aortae
title_full Electrical field stimulation-induced contractions on Pantherophis guttatus corpora cavernosa and aortae
title_fullStr Electrical field stimulation-induced contractions on Pantherophis guttatus corpora cavernosa and aortae
title_full_unstemmed Electrical field stimulation-induced contractions on Pantherophis guttatus corpora cavernosa and aortae
title_short Electrical field stimulation-induced contractions on Pantherophis guttatus corpora cavernosa and aortae
title_sort electrical field stimulation-induced contractions on pantherophis guttatus corpora cavernosa and aortae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5908133/
https://www.ncbi.nlm.nih.gov/pubmed/29672643
http://dx.doi.org/10.1371/journal.pone.0196123
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