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Esophageal squamous cell carcinoma transcriptome reveals the effect of FOXM1 on patient outcome through novel PIK3R3 mediated activation of PI3K signaling pathway
Esophageal squamous cell carcinoma (ESCC) presents poor prognosis, and patients diagnosed with this tumor currently lack target treatments. Therefore, in order to identify potential targets for ESCC treatment, we carried out a transcriptome analysis with ESCC and paired nonmalignant surrounding muco...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5908275/ https://www.ncbi.nlm.nih.gov/pubmed/29682174 http://dx.doi.org/10.18632/oncotarget.24621 |
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author | Nicolau-Neto, Pedro Da Costa, Nathalia Meireles de Souza Santos, Paulo Thiago Gonzaga, Isabela Martins Ferreira, Maria Aparecida Guaraldi, Simone Moreira, Miguel Angelo Seuánez, Hector N. Brewer, Lilian Bergmann, Anke Boroni, Mariana Mencalha, Andre Luiz Kruel, Cleber Dario Pinto Lima, Sheila Coelho Soares Esposito, Dominic Simão, Tatiana Almeida Pinto, Luis Felipe Ribeiro |
author_facet | Nicolau-Neto, Pedro Da Costa, Nathalia Meireles de Souza Santos, Paulo Thiago Gonzaga, Isabela Martins Ferreira, Maria Aparecida Guaraldi, Simone Moreira, Miguel Angelo Seuánez, Hector N. Brewer, Lilian Bergmann, Anke Boroni, Mariana Mencalha, Andre Luiz Kruel, Cleber Dario Pinto Lima, Sheila Coelho Soares Esposito, Dominic Simão, Tatiana Almeida Pinto, Luis Felipe Ribeiro |
author_sort | Nicolau-Neto, Pedro |
collection | PubMed |
description | Esophageal squamous cell carcinoma (ESCC) presents poor prognosis, and patients diagnosed with this tumor currently lack target treatments. Therefore, in order to identify potential targets for ESCC treatment, we carried out a transcriptome analysis with ESCC and paired nonmalignant surrounding mucosa samples, followed by a master regulator analysis, and further explored the role of the identified central regulatory genes through in vivo and in vitro assays. Among the transcription factors deregulated/enriched in ESCC, we focused on FOXM1 because of its involvement in the regulation of critical biological processes. A new transcriptome analysis performed with ESCC cell lineage TE-1 showed that the modulation of FOXM1 expression resulted in PIK3R3 expression changes, whereas chromatin immunoprecipitation assay revealed that FOXM1 was capable of binding onto PIK3R3 promoter, thus demonstrating that PIK3R3 is a new FOXM1 target. Furthermore, FOXM1 overexpression resulted in the activation of PIK3/AKT signaling pathway through PIK3R3-mediated AKT phosphorylation. Finally, the analysis of the clinic-pathological data of ESCC patients revealed that overexpression of both FOXM1 and PIK3R3 was associated with poor prognosis, but only the latter was an independent prognosis factor for ESCC patients. In conclusion, our results show that FOXM1 seems to play a central role in ESCC carcinogenesis by upregulating many oncogenes found overexpressed in this tumor. Furthermore, PIK3R3 is a novel FOXM1 target that triggers the activation of the PI3K/AKT pathway in ESCC cells. |
format | Online Article Text |
id | pubmed-5908275 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-59082752018-04-20 Esophageal squamous cell carcinoma transcriptome reveals the effect of FOXM1 on patient outcome through novel PIK3R3 mediated activation of PI3K signaling pathway Nicolau-Neto, Pedro Da Costa, Nathalia Meireles de Souza Santos, Paulo Thiago Gonzaga, Isabela Martins Ferreira, Maria Aparecida Guaraldi, Simone Moreira, Miguel Angelo Seuánez, Hector N. Brewer, Lilian Bergmann, Anke Boroni, Mariana Mencalha, Andre Luiz Kruel, Cleber Dario Pinto Lima, Sheila Coelho Soares Esposito, Dominic Simão, Tatiana Almeida Pinto, Luis Felipe Ribeiro Oncotarget Research Paper Esophageal squamous cell carcinoma (ESCC) presents poor prognosis, and patients diagnosed with this tumor currently lack target treatments. Therefore, in order to identify potential targets for ESCC treatment, we carried out a transcriptome analysis with ESCC and paired nonmalignant surrounding mucosa samples, followed by a master regulator analysis, and further explored the role of the identified central regulatory genes through in vivo and in vitro assays. Among the transcription factors deregulated/enriched in ESCC, we focused on FOXM1 because of its involvement in the regulation of critical biological processes. A new transcriptome analysis performed with ESCC cell lineage TE-1 showed that the modulation of FOXM1 expression resulted in PIK3R3 expression changes, whereas chromatin immunoprecipitation assay revealed that FOXM1 was capable of binding onto PIK3R3 promoter, thus demonstrating that PIK3R3 is a new FOXM1 target. Furthermore, FOXM1 overexpression resulted in the activation of PIK3/AKT signaling pathway through PIK3R3-mediated AKT phosphorylation. Finally, the analysis of the clinic-pathological data of ESCC patients revealed that overexpression of both FOXM1 and PIK3R3 was associated with poor prognosis, but only the latter was an independent prognosis factor for ESCC patients. In conclusion, our results show that FOXM1 seems to play a central role in ESCC carcinogenesis by upregulating many oncogenes found overexpressed in this tumor. Furthermore, PIK3R3 is a novel FOXM1 target that triggers the activation of the PI3K/AKT pathway in ESCC cells. Impact Journals LLC 2018-03-30 /pmc/articles/PMC5908275/ /pubmed/29682174 http://dx.doi.org/10.18632/oncotarget.24621 Text en Copyright: © 2018 Nicolau-Neto et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Research Paper Nicolau-Neto, Pedro Da Costa, Nathalia Meireles de Souza Santos, Paulo Thiago Gonzaga, Isabela Martins Ferreira, Maria Aparecida Guaraldi, Simone Moreira, Miguel Angelo Seuánez, Hector N. Brewer, Lilian Bergmann, Anke Boroni, Mariana Mencalha, Andre Luiz Kruel, Cleber Dario Pinto Lima, Sheila Coelho Soares Esposito, Dominic Simão, Tatiana Almeida Pinto, Luis Felipe Ribeiro Esophageal squamous cell carcinoma transcriptome reveals the effect of FOXM1 on patient outcome through novel PIK3R3 mediated activation of PI3K signaling pathway |
title | Esophageal squamous cell carcinoma transcriptome reveals the effect of FOXM1 on patient outcome through novel PIK3R3 mediated activation of PI3K signaling pathway |
title_full | Esophageal squamous cell carcinoma transcriptome reveals the effect of FOXM1 on patient outcome through novel PIK3R3 mediated activation of PI3K signaling pathway |
title_fullStr | Esophageal squamous cell carcinoma transcriptome reveals the effect of FOXM1 on patient outcome through novel PIK3R3 mediated activation of PI3K signaling pathway |
title_full_unstemmed | Esophageal squamous cell carcinoma transcriptome reveals the effect of FOXM1 on patient outcome through novel PIK3R3 mediated activation of PI3K signaling pathway |
title_short | Esophageal squamous cell carcinoma transcriptome reveals the effect of FOXM1 on patient outcome through novel PIK3R3 mediated activation of PI3K signaling pathway |
title_sort | esophageal squamous cell carcinoma transcriptome reveals the effect of foxm1 on patient outcome through novel pik3r3 mediated activation of pi3k signaling pathway |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5908275/ https://www.ncbi.nlm.nih.gov/pubmed/29682174 http://dx.doi.org/10.18632/oncotarget.24621 |
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